Tags

Type your tag names separated by a space and hit enter

Dihydrodiol dehydrogenases in guinea pig liver.
Biochem Pharmacol. 1986 Nov 15; 35(22):4005-12.BP

Abstract

Four major and four minor dihydrodiol dehydrogenases, with similar apparent molecular weights of 28,000 to 34,000 but with different charges, were purified from male guinea pig liver cytosol. One of the minor enzymes catalyzed only the oxidation of benzene dihydrodiol with a high Km value of 5.0 mM and was identified immunologically with aldehyde reductase. The other enzymes oxidized xenobiotic alicyclic alcohols and 17 beta-hydroxysteroids as well as benzene dihydrodiol. These enzymes exhibited higher affinity for 17 beta-hydroxysteroids than for alicyclic alcohols and benzene dihydrodiol, and immunologically cross-reacted with testosterone 17 beta-dehydrogenase purified from the same source. Four major enzymes and one minor with Km values for benzene dihydrodiol of about 0.2 mM, possessed specificity for 5 beta-androstane--17 beta-hydroxysteroids and dual cofactor requirement, whereas the other two minor enzymes with high Km values of over 5 mM showed apparent NADP and 5 alpha-androstane specificity. The dihydrodiol dehydrogenase activity was localized in the cytosol of liver. The results indicate that the hepatic oxidation of dihydrodiols in the guinea pig is mediated by cytosolic testosterone 17 beta-dehydrogenase isozymes and aldehyde reductase. Testosterone 17 beta-dehydrogenase immunologically identical to the liver enzymes was detected only in kidney, whereas aldehyde reductase was detected in all tissues of the guinea pig.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

3535806

Citation

Hara, A, et al. "Dihydrodiol Dehydrogenases in Guinea Pig Liver." Biochemical Pharmacology, vol. 35, no. 22, 1986, pp. 4005-12.
Hara A, Hasebe K, Hayashibara M, et al. Dihydrodiol dehydrogenases in guinea pig liver. Biochem Pharmacol. 1986;35(22):4005-12.
Hara, A., Hasebe, K., Hayashibara, M., Matsuura, K., Nakayama, T., & Sawada, H. (1986). Dihydrodiol dehydrogenases in guinea pig liver. Biochemical Pharmacology, 35(22), 4005-12.
Hara A, et al. Dihydrodiol Dehydrogenases in Guinea Pig Liver. Biochem Pharmacol. 1986 Nov 15;35(22):4005-12. PubMed PMID: 3535806.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dihydrodiol dehydrogenases in guinea pig liver. AU - Hara,A, AU - Hasebe,K, AU - Hayashibara,M, AU - Matsuura,K, AU - Nakayama,T, AU - Sawada,H, PY - 1986/11/15/pubmed PY - 1986/11/15/medline PY - 1986/11/15/entrez SP - 4005 EP - 12 JF - Biochemical pharmacology JO - Biochem. Pharmacol. VL - 35 IS - 22 N2 - Four major and four minor dihydrodiol dehydrogenases, with similar apparent molecular weights of 28,000 to 34,000 but with different charges, were purified from male guinea pig liver cytosol. One of the minor enzymes catalyzed only the oxidation of benzene dihydrodiol with a high Km value of 5.0 mM and was identified immunologically with aldehyde reductase. The other enzymes oxidized xenobiotic alicyclic alcohols and 17 beta-hydroxysteroids as well as benzene dihydrodiol. These enzymes exhibited higher affinity for 17 beta-hydroxysteroids than for alicyclic alcohols and benzene dihydrodiol, and immunologically cross-reacted with testosterone 17 beta-dehydrogenase purified from the same source. Four major enzymes and one minor with Km values for benzene dihydrodiol of about 0.2 mM, possessed specificity for 5 beta-androstane--17 beta-hydroxysteroids and dual cofactor requirement, whereas the other two minor enzymes with high Km values of over 5 mM showed apparent NADP and 5 alpha-androstane specificity. The dihydrodiol dehydrogenase activity was localized in the cytosol of liver. The results indicate that the hepatic oxidation of dihydrodiols in the guinea pig is mediated by cytosolic testosterone 17 beta-dehydrogenase isozymes and aldehyde reductase. Testosterone 17 beta-dehydrogenase immunologically identical to the liver enzymes was detected only in kidney, whereas aldehyde reductase was detected in all tissues of the guinea pig. SN - 0006-2952 UR - https://www.unboundmedicine.com/medline/citation/3535806/Dihydrodiol_dehydrogenases_in_guinea_pig_liver_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0006-2952(86)90019-5 DB - PRIME DP - Unbound Medicine ER -