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Knockout of OsNRAMP5 enhances rice tolerance to cadmium toxicity in response to varying external cadmium concentrations via distinct mechanisms.
Sci Total Environ. 2022 Aug 01; 832:155006.ST

Abstract

OsNRAMP5 is a transporter responsible for cadmium (Cd) and manganese (Mn) uptake and root-to-shoot translocation of Mn in rice plants. Knockout of OsNRAMP5 is regarded as an effective approach to minimize Cd uptake and accumulation in rice. It is vital to evaluate the effects of knocking out OsNRAMP5 on Cd and Mn accumulation, as well as Cd tolerance of rice plants in response to varying environmental Cd concentrations, and to uncover the underlying mechanism, which until now, has remained largely unexplored. This study showed that knockout of OsNRAMP5 decreased Cd uptake, but simultaneously facilitated Cd translocation from roots to shoots. The effect of OsNRAMP5 knockout on reducing root Cd uptake weakened, however its effect on improving root-to-shoot Cd translocation was constant with increasing environmental Cd concentrations. As a result, its mutation dramatically reduced Cd accumulation in shoots under low and moderate Cd stress, but inversely increased that under high Cd conditions. Interestingly, Cd tolerance of its knockout mutants was persistently enhanced, irrespective of lower or higher Cd concentrations in shoots, compared with that of wild-type plants. Knockout of OsNRAMP5 mitigated Cd toxicity by dramatically diminishing Cd uptake at low or moderate external Cd concentrations. Remarkably, its knockout effectively complemented deficient mineral nutrients in shoots, thereby indirectly enhancing rice tolerance to severe Cd stress. Additionally, its mutation conferred preferential delivery of Mn to young leaves and grains. These results have important implications for the application of the OsNRAMP5 mutation in mitigating Cd toxicity and lowering the risk of excessive Cd accumulation in rice grains.

Authors+Show Affiliations

State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, Changsha 410125, China; Longping Branch of Graduate School, Hunan University, Changsha 410125, China.Longping Branch of Graduate School, Hunan University, Changsha 410125, China.Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China.State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, Changsha 410125, China; Longping Branch of Graduate School, Hunan University, Changsha 410125, China.Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China.Key Laboratory of Agro-Ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China.State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, Changsha 410125, China.State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, Changsha 410125, China.Longping Branch of Graduate School, Hunan University, Changsha 410125, China.State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, Changsha 410125, China; Longping Branch of Graduate School, Hunan University, Changsha 410125, China.State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, Changsha 410125, China.State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, Changsha 410125, China.State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, Changsha 410125, China; Longping Branch of Graduate School, Hunan University, Changsha 410125, China. Electronic address: brzhaorice@163.com.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

35381246

Citation

Tang, Li, et al. "Knockout of OsNRAMP5 Enhances Rice Tolerance to Cadmium Toxicity in Response to Varying External Cadmium Concentrations Via Distinct Mechanisms." The Science of the Total Environment, vol. 832, 2022, p. 155006.
Tang L, Dong J, Qu M, et al. Knockout of OsNRAMP5 enhances rice tolerance to cadmium toxicity in response to varying external cadmium concentrations via distinct mechanisms. Sci Total Environ. 2022;832:155006.
Tang, L., Dong, J., Qu, M., Lv, Q., Zhang, L., Peng, C., Hu, Y., Li, Y., Ji, Z., Mao, B., Peng, Y., Shao, Y., & Zhao, B. (2022). Knockout of OsNRAMP5 enhances rice tolerance to cadmium toxicity in response to varying external cadmium concentrations via distinct mechanisms. The Science of the Total Environment, 832, 155006. https://doi.org/10.1016/j.scitotenv.2022.155006
Tang L, et al. Knockout of OsNRAMP5 Enhances Rice Tolerance to Cadmium Toxicity in Response to Varying External Cadmium Concentrations Via Distinct Mechanisms. Sci Total Environ. 2022 Aug 1;832:155006. PubMed PMID: 35381246.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Knockout of OsNRAMP5 enhances rice tolerance to cadmium toxicity in response to varying external cadmium concentrations via distinct mechanisms. AU - Tang,Li, AU - Dong,Jiayu, AU - Qu,Mengmeng, AU - Lv,Qiming, AU - Zhang,Liping, AU - Peng,Can, AU - Hu,Yuanyi, AU - Li,Yaokui, AU - Ji,Zhongying, AU - Mao,Bigang, AU - Peng,Yan, AU - Shao,Ye, AU - Zhao,Bingran, Y1 - 2022/04/02/ PY - 2021/11/02/received PY - 2022/03/28/revised PY - 2022/03/30/accepted PY - 2022/4/6/pubmed PY - 2022/4/6/medline PY - 2022/4/5/entrez KW - Cadmium toxicity KW - Manganese KW - Metal transporter KW - OsNRAMP5 KW - Rice (Oryza sativa L.) SP - 155006 EP - 155006 JF - The Science of the total environment JO - Sci Total Environ VL - 832 N2 - OsNRAMP5 is a transporter responsible for cadmium (Cd) and manganese (Mn) uptake and root-to-shoot translocation of Mn in rice plants. Knockout of OsNRAMP5 is regarded as an effective approach to minimize Cd uptake and accumulation in rice. It is vital to evaluate the effects of knocking out OsNRAMP5 on Cd and Mn accumulation, as well as Cd tolerance of rice plants in response to varying environmental Cd concentrations, and to uncover the underlying mechanism, which until now, has remained largely unexplored. This study showed that knockout of OsNRAMP5 decreased Cd uptake, but simultaneously facilitated Cd translocation from roots to shoots. The effect of OsNRAMP5 knockout on reducing root Cd uptake weakened, however its effect on improving root-to-shoot Cd translocation was constant with increasing environmental Cd concentrations. As a result, its mutation dramatically reduced Cd accumulation in shoots under low and moderate Cd stress, but inversely increased that under high Cd conditions. Interestingly, Cd tolerance of its knockout mutants was persistently enhanced, irrespective of lower or higher Cd concentrations in shoots, compared with that of wild-type plants. Knockout of OsNRAMP5 mitigated Cd toxicity by dramatically diminishing Cd uptake at low or moderate external Cd concentrations. Remarkably, its knockout effectively complemented deficient mineral nutrients in shoots, thereby indirectly enhancing rice tolerance to severe Cd stress. Additionally, its mutation conferred preferential delivery of Mn to young leaves and grains. These results have important implications for the application of the OsNRAMP5 mutation in mitigating Cd toxicity and lowering the risk of excessive Cd accumulation in rice grains. SN - 1879-1026 UR - https://www.unboundmedicine.com/medline/citation/35381246/Knockout_of_OsNRAMP5_enhances_rice_tolerance_to_cadmium_toxicity_in_response_to_varying_external_cadmium_concentrations_via_distinct_mechanisms_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0048-9697(22)02099-X DB - PRIME DP - Unbound Medicine ER -