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Ecofriendly phytofabrication of silver nanoparticles using aqueous extract of Cuphea carthagenensis and their antioxidant potential and antibacterial activity against clinically important human pathogens.
Chemosphere. 2022 Aug; 300:134497.C

Abstract

The green synthesis of nanoparticles (NPs) is the safest, ecofriendly, cost-effective, and non-hazardous approach of nanotechnology. In the current study, we described the green synthesis of silver nanoparticles (AgNPs) using Cuphea carthagenensis aqueous leaf extract as a reducing, capping, and stabilizing agent. The study aims at the synthesis, characterization, optimization, and determination of the antibacterial activity of Cc-AgNPs against clinically important human pathogens. Coating of cotton fabrics with Cc-AgNPs and their efficacy against skin infection causing organisms was also evaluated. Furthermore, antioxidant activity, growth assay and time kill assay of Cc-AgNPs were also performed in the study. The biosynthesized Cc-AgNPs were characterized by UV-visible spectrometry, energy-dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), transmission electron microscopy (TEM), and Fourier transform infrared spectroscopy (FTIR). The spectroscopic and microscopic analysis demonstrated biosynthesis of face-centered cubic (fcc) crystalline spherical Cc-AgNPs with an average particle size of 10.65 ± 0.1 nm. Optimized peak synthesis of Cc-AgNPs was reported at pH7, 55 °C, 4 mM silver nitrate, and 5:45 (plant extract: silver nitrate). Cc-AgNPs exhibited potent antioxidant effect and antibacterial activity against both Gram-positive and Gram-negative bacteria. The lowest MIC (15 μg/ml) and MBC (25 μg/ml) values were reported against S. typhimurium. The Cc-AgNPs coated fabrics demonstrated potent antibacterial activity against tested strains. This application could be helpful in wound healing management. Furthermore, the hemolytic analysis demonstrated that Cc-AgNPs exhibit non-toxic nature against Red Blood Cells (RBCs) at the tested concentrations. In conclusion, the investigation demonstrated a fast, stable, and eco-friendly approach to the biosynthesis of Cc-AgNPs along with their antibacterial and antioxidant properties.

Authors+Show Affiliations

Department of Molecular Biology and Biotechnology, Tezpur University, Napaam, Tezpur, 784028, Assam, India.Department of Molecular Biology and Biotechnology, Tezpur University, Napaam, Tezpur, 784028, Assam, India.Department of Molecular Biology and Biotechnology, Tezpur University, Napaam, Tezpur, 784028, Assam, India.Department of Energy, Tezpur University, Napaam, Tezpur, 784028, Assam, India.Department of Energy, Tezpur University, Napaam, Tezpur, 784028, Assam, India.Environmental Biotechnology and Genomics Division (EBGD) CSIR-NEERI, Nagpur, 440020, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, Delhi, India.Department of Veterinary Microbiology CVSc & AH, Central Agricultural University Selesih, Aizawl, Mizoram, 796014, India.Department of Veterinary Microbiology, West Bengal University of Animal & Fishery Sciences 68, K. B. Sarani, Kolkata, 700037, India.Department of Molecular Biology and Biotechnology, Tezpur University, Napaam, Tezpur, 784028, Assam, India. Electronic address: mandal@tezu.ernet.in.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

35398470

Citation

Rather, Muzamil Ahmad, et al. "Ecofriendly Phytofabrication of Silver Nanoparticles Using Aqueous Extract of Cuphea Carthagenensis and Their Antioxidant Potential and Antibacterial Activity Against Clinically Important Human Pathogens." Chemosphere, vol. 300, 2022, p. 134497.
Rather MA, Deori PJ, Gupta K, et al. Ecofriendly phytofabrication of silver nanoparticles using aqueous extract of Cuphea carthagenensis and their antioxidant potential and antibacterial activity against clinically important human pathogens. Chemosphere. 2022;300:134497.
Rather, M. A., Deori, P. J., Gupta, K., Daimary, N., Deka, D., Qureshi, A., Dutta, T. K., Joardar, S. N., & Mandal, M. (2022). Ecofriendly phytofabrication of silver nanoparticles using aqueous extract of Cuphea carthagenensis and their antioxidant potential and antibacterial activity against clinically important human pathogens. Chemosphere, 300, 134497. https://doi.org/10.1016/j.chemosphere.2022.134497
Rather MA, et al. Ecofriendly Phytofabrication of Silver Nanoparticles Using Aqueous Extract of Cuphea Carthagenensis and Their Antioxidant Potential and Antibacterial Activity Against Clinically Important Human Pathogens. Chemosphere. 2022;300:134497. PubMed PMID: 35398470.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ecofriendly phytofabrication of silver nanoparticles using aqueous extract of Cuphea carthagenensis and their antioxidant potential and antibacterial activity against clinically important human pathogens. AU - Rather,Muzamil Ahmad, AU - Deori,Pranab Jyoti, AU - Gupta,Kuldeep, AU - Daimary,Niran, AU - Deka,Dhanapati, AU - Qureshi,Asifa, AU - Dutta,Tapan Kumar, AU - Joardar,Siddhartha Narayan, AU - Mandal,Manabendra, Y1 - 2022/04/07/ PY - 2022/02/05/received PY - 2022/03/24/revised PY - 2022/03/31/accepted PY - 2022/4/11/pubmed PY - 2022/4/11/medline PY - 2022/4/10/entrez KW - Antibacterial KW - Antioxidant KW - Cuphea carthagenensis, green synthesis KW - Pathogen KW - Silver nanoparticles SP - 134497 EP - 134497 JF - Chemosphere JO - Chemosphere VL - 300 N2 - The green synthesis of nanoparticles (NPs) is the safest, ecofriendly, cost-effective, and non-hazardous approach of nanotechnology. In the current study, we described the green synthesis of silver nanoparticles (AgNPs) using Cuphea carthagenensis aqueous leaf extract as a reducing, capping, and stabilizing agent. The study aims at the synthesis, characterization, optimization, and determination of the antibacterial activity of Cc-AgNPs against clinically important human pathogens. Coating of cotton fabrics with Cc-AgNPs and their efficacy against skin infection causing organisms was also evaluated. Furthermore, antioxidant activity, growth assay and time kill assay of Cc-AgNPs were also performed in the study. The biosynthesized Cc-AgNPs were characterized by UV-visible spectrometry, energy-dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), transmission electron microscopy (TEM), and Fourier transform infrared spectroscopy (FTIR). The spectroscopic and microscopic analysis demonstrated biosynthesis of face-centered cubic (fcc) crystalline spherical Cc-AgNPs with an average particle size of 10.65 ± 0.1 nm. Optimized peak synthesis of Cc-AgNPs was reported at pH7, 55 °C, 4 mM silver nitrate, and 5:45 (plant extract: silver nitrate). Cc-AgNPs exhibited potent antioxidant effect and antibacterial activity against both Gram-positive and Gram-negative bacteria. The lowest MIC (15 μg/ml) and MBC (25 μg/ml) values were reported against S. typhimurium. The Cc-AgNPs coated fabrics demonstrated potent antibacterial activity against tested strains. This application could be helpful in wound healing management. Furthermore, the hemolytic analysis demonstrated that Cc-AgNPs exhibit non-toxic nature against Red Blood Cells (RBCs) at the tested concentrations. In conclusion, the investigation demonstrated a fast, stable, and eco-friendly approach to the biosynthesis of Cc-AgNPs along with their antibacterial and antioxidant properties. SN - 1879-1298 UR - https://www.unboundmedicine.com/medline/citation/35398470/Ecofriendly_phytofabrication_of_silver_nanoparticles_using_aqueous_extract_of_Cuphea_carthagenensis_and_their_antioxidant_potential_and_antibacterial_activity_against_clinically_important_human_pathogens_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0045-6535(22)00990-0 DB - PRIME DP - Unbound Medicine ER -