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Advantage of Fontana-Masson stain in capsule-deficient cryptococcal infection.
Arch Pathol Lab Med. 1987 Jan; 111(1):53-7.AP

Abstract

We describe light and electron microscopic findings in three cases of pulmonary infection caused by capsule-deficient Cryptococcus neoformans (CDCN) and emphasize the value of the Fontana-Masson silver (FMS) stain in diagnosing cryptococcosis. Capsule-deficient C neoformans infections, including coccidioidomycosis, histoplasmosis, typical cryptococcosis, blastomycosis, candidiasis, and pneumocystosis were used as controls. Several stains including mucicarmine, alcian blue, and FMS were done on tissue sections from all of the above mentioned infections. It was found that while the FMS stain was positive only in the organisms of CDCN and typical C neoformans, the mucicarmine was positive only in typical C neoformans. The negativity of the mucicarmine in the organisms of CDCN reflected the absence of capsular material as demonstrated by ultrastructural study. Therefore, when fungal cultures are not available or are negative, and tissue mucicarmine is negative, the FMS stain is a valuable alternative for use on tissue sections. It may be used, along with routine fungal stains, when a yeast-forming fungal infection is suspected.

Authors

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Pub Type(s)

Journal Article

Language

eng

PubMed ID

3541845

Citation

Ro, J Y., et al. "Advantage of Fontana-Masson Stain in Capsule-deficient Cryptococcal Infection." Archives of Pathology & Laboratory Medicine, vol. 111, no. 1, 1987, pp. 53-7.
Ro JY, Lee SS, Ayala AG. Advantage of Fontana-Masson stain in capsule-deficient cryptococcal infection. Arch Pathol Lab Med. 1987;111(1):53-7.
Ro, J. Y., Lee, S. S., & Ayala, A. G. (1987). Advantage of Fontana-Masson stain in capsule-deficient cryptococcal infection. Archives of Pathology & Laboratory Medicine, 111(1), 53-7.
Ro JY, Lee SS, Ayala AG. Advantage of Fontana-Masson Stain in Capsule-deficient Cryptococcal Infection. Arch Pathol Lab Med. 1987;111(1):53-7. PubMed PMID: 3541845.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Advantage of Fontana-Masson stain in capsule-deficient cryptococcal infection. AU - Ro,J Y, AU - Lee,S S, AU - Ayala,A G, PY - 1987/1/1/pubmed PY - 1987/1/1/medline PY - 1987/1/1/entrez SP - 53 EP - 7 JF - Archives of pathology & laboratory medicine JO - Arch Pathol Lab Med VL - 111 IS - 1 N2 - We describe light and electron microscopic findings in three cases of pulmonary infection caused by capsule-deficient Cryptococcus neoformans (CDCN) and emphasize the value of the Fontana-Masson silver (FMS) stain in diagnosing cryptococcosis. Capsule-deficient C neoformans infections, including coccidioidomycosis, histoplasmosis, typical cryptococcosis, blastomycosis, candidiasis, and pneumocystosis were used as controls. Several stains including mucicarmine, alcian blue, and FMS were done on tissue sections from all of the above mentioned infections. It was found that while the FMS stain was positive only in the organisms of CDCN and typical C neoformans, the mucicarmine was positive only in typical C neoformans. The negativity of the mucicarmine in the organisms of CDCN reflected the absence of capsular material as demonstrated by ultrastructural study. Therefore, when fungal cultures are not available or are negative, and tissue mucicarmine is negative, the FMS stain is a valuable alternative for use on tissue sections. It may be used, along with routine fungal stains, when a yeast-forming fungal infection is suspected. SN - 0003-9985 UR - https://www.unboundmedicine.com/medline/citation/3541845/Advantage_of_Fontana_Masson_stain_in_capsule_deficient_cryptococcal_infection_ DB - PRIME DP - Unbound Medicine ER -