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Characterization of Silver Nanoparticles Synthesized by Leaves of Lonicera japonica Thunb.
Int J Nanomedicine. 2022; 17:1647-1657.IJ

Abstract

Background

The leaves of L. japonica (LLJ) are widely used as medicine in China. It is rich in caffeoylquinic acids, flavonoids and iridoid glycosides and has strong reducing capacities. Therefore, it can be used as a green material to synthesize silver nanoparticles.

Methods

LLJ was used as a reducing agent to produce the LLJ-mediated silver nanoparticles (LLJ-AgNPs). The structure and physicochemical properties of LLJ-AgNPs were characterized by ultraviolet spectroscopy (UV-Vis), scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and x-ray powder diffraction (XRD). Antioxidant activity of LLJ-AgNPs was determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging. Antibacterial activity was determined by 96 well plates (AGAR) gradient dilution, while the anticancer potential was determined by MTT assay.

Results

The results showed LLJ-AgNPs had a spherical structure with the maximum UV-Vis absorption at 400 nm. In addition, LLJ-AgNPs exhibited excellent antioxidant properties, where the free radical scavenging rate of LLJ-AgNPs was increased from 39% to 92% at concentrations from 0.25 to 1.0 mg/mL. Moreover, LLJ-AgNPs displayed excellent antibacterial properties against E. coli and Salmonella at room temperature, with minimum inhibitory values of 10-6 and 10-5 g/L, respectively. In addition, the synthetic LLJ-AgNPs exhibited a better inhibition effect in the proliferation of cancer cells (HepG2, MDA-MB -231, and Hela cells).

Conclusion

The present study provides a green approach to synthesize LLJ-AgNPs. All those findings illustrated that the produced LLJ-AgNPs can be used as an economical and efficient functional material for further applications in food and pharmaceutical fields.

Authors+Show Affiliations

School of Chemistry and Chemical Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250353, People's Republic of China. School of Pharmaceutical Sciences and Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250014, People's Republic of China. Biological Engineering Technology Innovation Center of Shandong Province, Heze Branch of Qilu University of Technology (Shandong Academy of Sciences), Heze, 274000, People's Republic of China.School of Pharmaceutical Sciences and Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250014, People's Republic of China.Shandong Provincial Center of Forest and Grass Germplasm Resources, Jinan, 250102, People's Republic of China.School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan, 250353, People's Republic of China.Department of Bioorganic Chemistry, Leibniz Institute of Plant BioChemistry, Halle, D-06120, Germany.Shandong Provincial Center of Forest and Grass Germplasm Resources, Jinan, 250102, People's Republic of China.School of Pharmaceutical Sciences and Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250014, People's Republic of China.Shandong Hongjitang Pharmaceutical Group Co., Ltd., Jinan, 250103, People's Republic of China.Shandong Hongjitang Pharmaceutical Group Co., Ltd., Jinan, 250103, People's Republic of China.School of Chemistry and Chemical Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250353, People's Republic of China.School of Pharmaceutical Sciences and Key Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250014, People's Republic of China. Biological Engineering Technology Innovation Center of Shandong Province, Heze Branch of Qilu University of Technology (Shandong Academy of Sciences), Heze, 274000, People's Republic of China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

35418754

Citation

Zhang, Yu, et al. "Characterization of Silver Nanoparticles Synthesized By Leaves of Lonicera Japonica Thunb." International Journal of Nanomedicine, vol. 17, 2022, pp. 1647-1657.
Zhang Y, Cui L, Lu Y, et al. Characterization of Silver Nanoparticles Synthesized by Leaves of Lonicera japonica Thunb. Int J Nanomedicine. 2022;17:1647-1657.
Zhang, Y., Cui, L., Lu, Y., He, J., Hussain, H., Xie, L., Sun, X., Meng, Z., Cao, G., Qin, D., & Wang, D. (2022). Characterization of Silver Nanoparticles Synthesized by Leaves of Lonicera japonica Thunb. International Journal of Nanomedicine, 17, 1647-1657. https://doi.org/10.2147/IJN.S356919
Zhang Y, et al. Characterization of Silver Nanoparticles Synthesized By Leaves of Lonicera Japonica Thunb. Int J Nanomedicine. 2022;17:1647-1657. PubMed PMID: 35418754.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of Silver Nanoparticles Synthesized by Leaves of Lonicera japonica Thunb. AU - Zhang,Yu, AU - Cui,Li, AU - Lu,Yizeng, AU - He,Jixiang, AU - Hussain,Hidayat, AU - Xie,Lei, AU - Sun,Xuan, AU - Meng,Zhaoqing, AU - Cao,Guiyun, AU - Qin,Dawei, AU - Wang,Daijie, Y1 - 2022/04/06/ PY - 2022/01/03/received PY - 2022/03/24/accepted PY - 2022/4/14/entrez PY - 2022/4/15/pubmed PY - 2022/4/16/medline KW - LLJ-AgNO3-NPs KW - antibacterial KW - anticancer KW - antioxidant KW - green approach KW - leaves of Lonicera japonica Thunb SP - 1647 EP - 1657 JF - International journal of nanomedicine JO - Int J Nanomedicine VL - 17 N2 - Background: The leaves of L. japonica (LLJ) are widely used as medicine in China. It is rich in caffeoylquinic acids, flavonoids and iridoid glycosides and has strong reducing capacities. Therefore, it can be used as a green material to synthesize silver nanoparticles. Methods: LLJ was used as a reducing agent to produce the LLJ-mediated silver nanoparticles (LLJ-AgNPs). The structure and physicochemical properties of LLJ-AgNPs were characterized by ultraviolet spectroscopy (UV-Vis), scanning electron microscopy (SEM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and x-ray powder diffraction (XRD). Antioxidant activity of LLJ-AgNPs was determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging. Antibacterial activity was determined by 96 well plates (AGAR) gradient dilution, while the anticancer potential was determined by MTT assay. Results: The results showed LLJ-AgNPs had a spherical structure with the maximum UV-Vis absorption at 400 nm. In addition, LLJ-AgNPs exhibited excellent antioxidant properties, where the free radical scavenging rate of LLJ-AgNPs was increased from 39% to 92% at concentrations from 0.25 to 1.0 mg/mL. Moreover, LLJ-AgNPs displayed excellent antibacterial properties against E. coli and Salmonella at room temperature, with minimum inhibitory values of 10-6 and 10-5 g/L, respectively. In addition, the synthetic LLJ-AgNPs exhibited a better inhibition effect in the proliferation of cancer cells (HepG2, MDA-MB -231, and Hela cells). Conclusion: The present study provides a green approach to synthesize LLJ-AgNPs. All those findings illustrated that the produced LLJ-AgNPs can be used as an economical and efficient functional material for further applications in food and pharmaceutical fields. SN - 1178-2013 UR - https://www.unboundmedicine.com/medline/citation/35418754/Characterization_of_Silver_Nanoparticles_Synthesized_by_Leaves_of_Lonicera_japonica_Thunb_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/35418754/ DB - PRIME DP - Unbound Medicine ER -