Tags

Type your tag names separated by a space and hit enter

Effects of exposure to NO2 on dome formation in alveolar epithelial cell monolayers.
Environ Res 1987; 42(1):1-11ER

Abstract

Primary cultured monolayers of rat Type II pneumocytes were used to investigate the effects of NO2 on alveolar epithelial barrier properties. Such monlayers form fluid-filled domes which are thought to result from active solute transport from medium to substratum, with water following passively. Using dome formation as a transport marker, 5-day-old cultures were directly exposed to 30 ppm NO2 in 5% CO2/air at 25 degrees C by cyclically tilting culture plates from side to side such that both halves of the monolayer were exposed during each cycle. Exposures consisted of 10 cycles of 4 min each (2 min per side), for a cell exposure time of 20 min. Control plates were simultaneously exposed to 5% CO2/air under identical conditions. Twenty-four hours after exposure, NO2-exposed monolayers exhibited significant decreases in dome density and individual dome volume as compared to controls. By 48 hr postexposure, differences between NO2-exposed and control values were less but remained significant. Control monolayers were essentially unaffected by exposure to 5% CO2/air. These results show that short-term sublethal exposures to NO2 produce a decrease in dome formation in Type II alveolar epithelial cell monolayers. This finding is most likely due to a decrease in the active transepithelial sodium transport rate and/or an increase in the permeability of cell membranes or tight junctions.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

3542522

Citation

Cheek, J M., et al. "Effects of Exposure to NO2 On Dome Formation in Alveolar Epithelial Cell Monolayers." Environmental Research, vol. 42, no. 1, 1987, pp. 1-11.
Cheek JM, Postlethwait EM, Shaw ME, et al. Effects of exposure to NO2 on dome formation in alveolar epithelial cell monolayers. Environ Res. 1987;42(1):1-11.
Cheek, J. M., Postlethwait, E. M., Shaw, M. E., & Crandall, E. D. (1987). Effects of exposure to NO2 on dome formation in alveolar epithelial cell monolayers. Environmental Research, 42(1), pp. 1-11.
Cheek JM, et al. Effects of Exposure to NO2 On Dome Formation in Alveolar Epithelial Cell Monolayers. Environ Res. 1987;42(1):1-11. PubMed PMID: 3542522.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of exposure to NO2 on dome formation in alveolar epithelial cell monolayers. AU - Cheek,J M, AU - Postlethwait,E M, AU - Shaw,M E, AU - Crandall,E D, PY - 1987/2/1/pubmed PY - 1987/2/1/medline PY - 1987/2/1/entrez SP - 1 EP - 11 JF - Environmental research JO - Environ. Res. VL - 42 IS - 1 N2 - Primary cultured monolayers of rat Type II pneumocytes were used to investigate the effects of NO2 on alveolar epithelial barrier properties. Such monlayers form fluid-filled domes which are thought to result from active solute transport from medium to substratum, with water following passively. Using dome formation as a transport marker, 5-day-old cultures were directly exposed to 30 ppm NO2 in 5% CO2/air at 25 degrees C by cyclically tilting culture plates from side to side such that both halves of the monolayer were exposed during each cycle. Exposures consisted of 10 cycles of 4 min each (2 min per side), for a cell exposure time of 20 min. Control plates were simultaneously exposed to 5% CO2/air under identical conditions. Twenty-four hours after exposure, NO2-exposed monolayers exhibited significant decreases in dome density and individual dome volume as compared to controls. By 48 hr postexposure, differences between NO2-exposed and control values were less but remained significant. Control monolayers were essentially unaffected by exposure to 5% CO2/air. These results show that short-term sublethal exposures to NO2 produce a decrease in dome formation in Type II alveolar epithelial cell monolayers. This finding is most likely due to a decrease in the active transepithelial sodium transport rate and/or an increase in the permeability of cell membranes or tight junctions. SN - 0013-9351 UR - https://www.unboundmedicine.com/medline/citation/3542522/Effects_of_exposure_to_NO2_on_dome_formation_in_alveolar_epithelial_cell_monolayers_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0013-9351(87)80002-6 DB - PRIME DP - Unbound Medicine ER -