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Development and application of specific FISH probes for karyotyping Psathyrostachys huashanica chromosomes.
BMC Genomics. 2022 Apr 18; 23(1):309.BG

Abstract

BACKGROUND

Psathyrostachys huashanica Keng has long been used as a genetic resource for improving wheat cultivar because of its genes mediating the resistance to various diseases (stripe rust, leaf rust, take-all, and powdery mildew) as well as its desirable agronomic traits. However, a high-resolution fluorescence in situ hybridization (FISH) karyotype of P. huashanica remains unavailable.

RESULTS

To develop chromosome-specific FISH markers for P. huashanica, repetitive sequences, including pSc119.2, pTa535, pTa713, pAs1, (AAC)5, (CTT)12, pSc200, pTa71A-2, and Oligo-44 were used for a FISH analysis. The results indicated that the combination of pSc200, pTa71A-2 and Oligo-44 probes can clearly identify all Ns genomic chromosomes in the two P. huashanica germplasms. The homoeologous relationships between individual P. huashanica chromosomes and common wheat chromosomes were clarified by FISH painting. Marker validation analyses revealed that the combination of pSc200, pTa71A-2, and Oligo-44 for a FISH analysis can distinguish the P. huashanica Ns-genome chromosomes from wheat chromosomes, as well as all chromosomes (except 4Ns) from the chromosomes of diploid wheat relatives carrying St, E, V, I, P and R genomes. Additionally, the probes were applicable for discriminating between the P. huashanica Ns-genome chromosomes in all homologous groups and the corresponding chromosomes in Psathyrostachys juncea and most Leymus species containing the Ns genome. Furthermore, six wheat-P. huashanica chromosome addition lines (i.e., 2Ns, 3Ns, 4Ns, 7Ns chromosomes and chromosomal segments) were characterized using the newly developed FISH markers. Thus, these probes can rapidly and precisely detect P. huashanica alien chromosomes in the wheat background.

CONCLUSIONS

The FISH karyotype established in this study lays a solid foundation for the efficient identification of P. huashanica chromosomes in wheat genetic improvement programs.

Links

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Authors+Show Affiliations

Zhang H
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, 611130, Chengdu, Sichuan, China. Triticeae Research Institute, Sichuan Agricultural University, 611130, Chengdu, Sichuan, China.
Wang F
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, 611130, Chengdu, Sichuan, China. Triticeae Research Institute, Sichuan Agricultural University, 611130, Chengdu, Sichuan, China.
Zeng C
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, 611130, Chengdu, Sichuan, China. Triticeae Research Institute, Sichuan Agricultural University, 611130, Chengdu, Sichuan, China.
Zhu W
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, 611130, Chengdu, Sichuan, China. Triticeae Research Institute, Sichuan Agricultural University, 611130, Chengdu, Sichuan, China.
Xu L
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, 611130, Chengdu, Sichuan, China.
Wang Y
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, 611130, Chengdu, Sichuan, China. Triticeae Research Institute, Sichuan Agricultural University, 611130, Chengdu, Sichuan, China.
Zeng J
College of Resources, Sichuan Agricultural University, 611130, Chengdu, Sichuan, China.
Fan X
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, 611130, Chengdu, Sichuan, China. Triticeae Research Institute, Sichuan Agricultural University, 611130, Chengdu, Sichuan, China.
Sha L
College of Grassland Science and Technology, Sichuan Agricultural University, 611130, Chengdu, Sichuan, China.
Wu D
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, 611130, Chengdu, Sichuan, China. Triticeae Research Institute, Sichuan Agricultural University, 611130, Chengdu, Sichuan, China.
Cheng Y
Triticeae Research Institute, Sichuan Agricultural University, 611130, Chengdu, Sichuan, China.
Zhang H
College of Grassland Science and Technology, Sichuan Agricultural University, 611130, Chengdu, Sichuan, China.
Chen G
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, 611130, Chengdu, Sichuan, China. Triticeae Research Institute, Sichuan Agricultural University, 611130, Chengdu, Sichuan, China.
Zhou Y
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, 611130, Chengdu, Sichuan, China. Triticeae Research Institute, Sichuan Agricultural University, 611130, Chengdu, Sichuan, China.
Kang H
State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, 611130, Chengdu, Sichuan, China. houyang.kang@sicau.edu.cn. Triticeae Research Institute, Sichuan Agricultural University, 611130, Chengdu, Sichuan, China. houyang.kang@sicau.edu.cn.

MeSH

Chromosomes, PlantDisease ResistanceGenetic MarkersIn Situ Hybridization, FluorescenceKaryotypingPlant DiseasesPoaceaeTriticum

Pub Type(s)

Journal Article

Language

eng

PubMed ID

35436853

Citation

Zhang, Hao, et al. "Development and Application of Specific FISH Probes for Karyotyping Psathyrostachys Huashanica Chromosomes." BMC Genomics, vol. 23, no. 1, 2022, p. 309.
Zhang H, Wang F, Zeng C, et al. Development and application of specific FISH probes for karyotyping Psathyrostachys huashanica chromosomes. BMC Genomics. 2022;23(1):309.
Zhang, H., Wang, F., Zeng, C., Zhu, W., Xu, L., Wang, Y., Zeng, J., Fan, X., Sha, L., Wu, D., Cheng, Y., Zhang, H., Chen, G., Zhou, Y., & Kang, H. (2022). Development and application of specific FISH probes for karyotyping Psathyrostachys huashanica chromosomes. BMC Genomics, 23(1), 309. https://doi.org/10.1186/s12864-022-08516-6
Zhang H, et al. Development and Application of Specific FISH Probes for Karyotyping Psathyrostachys Huashanica Chromosomes. BMC Genomics. 2022 Apr 18;23(1):309. PubMed PMID: 35436853.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development and application of specific FISH probes for karyotyping Psathyrostachys huashanica chromosomes. AU - Zhang,Hao, AU - Wang,Fei, AU - Zeng,Chunyan, AU - Zhu,Wei, AU - Xu,Lili, AU - Wang,Yi, AU - Zeng,Jian, AU - Fan,Xing, AU - Sha,Lina, AU - Wu,Dandan, AU - Cheng,Yiran, AU - Zhang,Haiqin, AU - Chen,Guoyue, AU - Zhou,Yonghong, AU - Kang,Houyang, Y1 - 2022/04/18/ PY - 2021/12/11/received PY - 2022/03/24/accepted PY - 2022/4/19/entrez PY - 2022/4/20/pubmed PY - 2022/4/21/medline KW - Addition lines KW - Chromosome identification KW - FISH painting KW - Fluorescence in situ hybridization karyotype KW - Psathyrostachys huashanica SP - 309 EP - 309 JF - BMC genomics JO - BMC Genomics VL - 23 IS - 1 N2 - BACKGROUND: Psathyrostachys huashanica Keng has long been used as a genetic resource for improving wheat cultivar because of its genes mediating the resistance to various diseases (stripe rust, leaf rust, take-all, and powdery mildew) as well as its desirable agronomic traits. However, a high-resolution fluorescence in situ hybridization (FISH) karyotype of P. huashanica remains unavailable. RESULTS: To develop chromosome-specific FISH markers for P. huashanica, repetitive sequences, including pSc119.2, pTa535, pTa713, pAs1, (AAC)5, (CTT)12, pSc200, pTa71A-2, and Oligo-44 were used for a FISH analysis. The results indicated that the combination of pSc200, pTa71A-2 and Oligo-44 probes can clearly identify all Ns genomic chromosomes in the two P. huashanica germplasms. The homoeologous relationships between individual P. huashanica chromosomes and common wheat chromosomes were clarified by FISH painting. Marker validation analyses revealed that the combination of pSc200, pTa71A-2, and Oligo-44 for a FISH analysis can distinguish the P. huashanica Ns-genome chromosomes from wheat chromosomes, as well as all chromosomes (except 4Ns) from the chromosomes of diploid wheat relatives carrying St, E, V, I, P and R genomes. Additionally, the probes were applicable for discriminating between the P. huashanica Ns-genome chromosomes in all homologous groups and the corresponding chromosomes in Psathyrostachys juncea and most Leymus species containing the Ns genome. Furthermore, six wheat-P. huashanica chromosome addition lines (i.e., 2Ns, 3Ns, 4Ns, 7Ns chromosomes and chromosomal segments) were characterized using the newly developed FISH markers. Thus, these probes can rapidly and precisely detect P. huashanica alien chromosomes in the wheat background. CONCLUSIONS: The FISH karyotype established in this study lays a solid foundation for the efficient identification of P. huashanica chromosomes in wheat genetic improvement programs. SN - 1471-2164 UR - https://www.unboundmedicine.com/medline/citation/35436853/Development_and_application_of_specific_FISH_probes_for_karyotyping_Psathyrostachys_huashanica_chromosomes_ DB - PRIME DP - Unbound Medicine ER -
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