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Immunocytochemical and autoradiographic localization of GABAergic neurons in the goldfish retina.
J Comp Neurol 1987; 255(2):317-25JC

Abstract

The putative neurotransmitter gamma-aminobutyric acid (GABA) was localized in goldfish retina by using an antiserum directed against GABA itself. The same types of cells were stained with this antibody as were labelled with an antiserum directed against the synthesizing enzyme for GABA, glutamic acid decarboxylase. Stained neurites of these cells were located throughout the inner plexiform layer (IPL) but staining was more intense in the proximal IPL. The GABA-immunoreactive staining could be reduced or completely abolished by preabsorbing the primary antibody with GABA. Uptake of [3H]-GABA or the GABA agonist [3H]-muscimol was localized in GABA-stained retinas using light microscope autoradiography. These experiments demonstrated that all types of GABA-immunoreactive amacrine cells had high-affinity uptake mechanisms for both [3H]-GABA and -muscimol. Thirty percent of proximal inner nuclear layer (INL) and some cells in the ganglion cell layer (GCL) were labelled by all three GABAergic markers. Most GABA-immunoreactive amacrine cells were lightly labelled due to [3H]-GABA uptake but a few amacrines (Ab) were heavily labelled. These findings demonstrate that the autoradiographic localization of [3H]-GABA or [3H]-muscimol uptake and the immunocytochemical localization of GAD or GABA are appropriate methods for localizing GABAergic neurons in the retina. Few GABA-immunoreactive amacrine cells accumulated the putative amino acid transmitter [3H]-glycine, verifying that the goldfish retina contains distinct subpopulations of glycinergic and GABAergic amacrine cells.

Authors

No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

3546408

Citation

Ball, A K.. "Immunocytochemical and Autoradiographic Localization of GABAergic Neurons in the Goldfish Retina." The Journal of Comparative Neurology, vol. 255, no. 2, 1987, pp. 317-25.
Ball AK. Immunocytochemical and autoradiographic localization of GABAergic neurons in the goldfish retina. J Comp Neurol. 1987;255(2):317-25.
Ball, A. K. (1987). Immunocytochemical and autoradiographic localization of GABAergic neurons in the goldfish retina. The Journal of Comparative Neurology, 255(2), pp. 317-25.
Ball AK. Immunocytochemical and Autoradiographic Localization of GABAergic Neurons in the Goldfish Retina. J Comp Neurol. 1987 Jan 8;255(2):317-25. PubMed PMID: 3546408.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Immunocytochemical and autoradiographic localization of GABAergic neurons in the goldfish retina. A1 - Ball,A K, PY - 1987/1/8/pubmed PY - 1987/1/8/medline PY - 1987/1/8/entrez SP - 317 EP - 25 JF - The Journal of comparative neurology JO - J. Comp. Neurol. VL - 255 IS - 2 N2 - The putative neurotransmitter gamma-aminobutyric acid (GABA) was localized in goldfish retina by using an antiserum directed against GABA itself. The same types of cells were stained with this antibody as were labelled with an antiserum directed against the synthesizing enzyme for GABA, glutamic acid decarboxylase. Stained neurites of these cells were located throughout the inner plexiform layer (IPL) but staining was more intense in the proximal IPL. The GABA-immunoreactive staining could be reduced or completely abolished by preabsorbing the primary antibody with GABA. Uptake of [3H]-GABA or the GABA agonist [3H]-muscimol was localized in GABA-stained retinas using light microscope autoradiography. These experiments demonstrated that all types of GABA-immunoreactive amacrine cells had high-affinity uptake mechanisms for both [3H]-GABA and -muscimol. Thirty percent of proximal inner nuclear layer (INL) and some cells in the ganglion cell layer (GCL) were labelled by all three GABAergic markers. Most GABA-immunoreactive amacrine cells were lightly labelled due to [3H]-GABA uptake but a few amacrines (Ab) were heavily labelled. These findings demonstrate that the autoradiographic localization of [3H]-GABA or [3H]-muscimol uptake and the immunocytochemical localization of GAD or GABA are appropriate methods for localizing GABAergic neurons in the retina. Few GABA-immunoreactive amacrine cells accumulated the putative amino acid transmitter [3H]-glycine, verifying that the goldfish retina contains distinct subpopulations of glycinergic and GABAergic amacrine cells. SN - 0021-9967 UR - https://www.unboundmedicine.com/medline/citation/3546408/Immunocytochemical_and_autoradiographic_localization_of_GABAergic_neurons_in_the_goldfish_retina_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0021-9967&date=1987&volume=255&issue=2&spage=317 DB - PRIME DP - Unbound Medicine ER -