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Variations of subtelomeric tandem repeats and rDNA on chromosome 1RS arms in the genus Secale and 1BL.1RS translocations.
BMC Plant Biol. 2022 Apr 25; 22(1):212.BP

Abstract

BACKGROUND

The wheat-rye 1BL.1RS translocations have played an important role in common wheat breeding programs. Subtelomeric tandem repeats have been often used to investigate polymorphisms of 1RS arms, but further research about their organizations on the 1RS chromosome is needed.

RESULTS

162 1RS arms from a wild rye species (Secale strictum) and six cultivated rye accessions (Secale cereale L.) (81 plants), 102 1BL.1RS and one 1AL.1RS translocations were investigated using oligo probes Oligo-TaiI, Oligo-pSc119.2-1, Oligo-pTa71A-2, Oligo-pSc200 and Oligo-pSc250, which were derived from tandem repeats TaiI, pSc119.2, pTa71, pSc200 and pSc250, respectively. The variations of 1RS arms were revealed by signal intensity of probes Oligo-pSc119.2-1, Oligo-pTa71A-2, Oligo-pSc200 and Oligo-pSc250. Proliferation of rDNA sequences on the 1RS chromosomes was observed. According to the presence of probe signals, 34, 127 and 144 of the 162 1RS arms contained TaiI, pSc200 and pSc250, respectively, and all of them contained pSc119.2 and pTa71. Most of the 1RS arms in rye contained three kinds of subtelomeric tandem repeats, the combination of pSc119.2, pSc200 and pSc250 was most common, and only eight of them contained TaiI, pSc119.2, pSc200 and pSc250. All of the 1RS arms in 1BL.1RS and 1AL.1RS translocations contained pSc119.2, pTa71, pSc200 and pSc250, but the presence of the TaiI family was not observed.

CONCLUSION

New organizations of subtelomeric tandem repeats on 1RS were found, and they reflected new genetic variations of 1RS arms. These 1RS arms might contain abundant allelic diversity for agricultural traits. The narrow genetic base of 1RS arms in 1BL.1RS and 1AL.1RS translocations currently used in agriculture is seriously restricting their use in wheat breeding programs. This research has found new 1RS sources for the future restructuring of 1BL.1RS translocations. The allelic variations of these 1RS arms should be studied more intensely as they may enrich the genetic diversity of 1BL.1RS translocations.

Authors+Show Affiliations

College of Agronomy, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, Sichuan, China. Provincial Key Laboratory for Plant Genetics and Breeding, Wenjiang, Chengdu, 611130, Sichuan, China.College of Agronomy, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, Sichuan, China. Provincial Key Laboratory for Plant Genetics and Breeding, Wenjiang, Chengdu, 611130, Sichuan, China.College of Agronomy, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, Sichuan, China. Provincial Key Laboratory for Plant Genetics and Breeding, Wenjiang, Chengdu, 611130, Sichuan, China.College of Agronomy, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, Sichuan, China. fushulan@sicau.edu.cn. Provincial Key Laboratory for Plant Genetics and Breeding, Wenjiang, Chengdu, 611130, Sichuan, China. fushulan@sicau.edu.cn.College of Agronomy, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, Sichuan, China. zxtang@sicau.edu.cn. Provincial Key Laboratory for Plant Genetics and Breeding, Wenjiang, Chengdu, 611130, Sichuan, China. zxtang@sicau.edu.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

35468732

Citation

Luo, Jie, et al. "Variations of Subtelomeric Tandem Repeats and rDNA On Chromosome 1RS Arms in the Genus Secale and 1BL.1RS Translocations." BMC Plant Biology, vol. 22, no. 1, 2022, p. 212.
Luo J, Liao R, Duan Y, et al. Variations of subtelomeric tandem repeats and rDNA on chromosome 1RS arms in the genus Secale and 1BL.1RS translocations. BMC Plant Biol. 2022;22(1):212.
Luo, J., Liao, R., Duan, Y., Fu, S., & Tang, Z. (2022). Variations of subtelomeric tandem repeats and rDNA on chromosome 1RS arms in the genus Secale and 1BL.1RS translocations. BMC Plant Biology, 22(1), 212. https://doi.org/10.1186/s12870-022-03598-6
Luo J, et al. Variations of Subtelomeric Tandem Repeats and rDNA On Chromosome 1RS Arms in the Genus Secale and 1BL.1RS Translocations. BMC Plant Biol. 2022 Apr 25;22(1):212. PubMed PMID: 35468732.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Variations of subtelomeric tandem repeats and rDNA on chromosome 1RS arms in the genus Secale and 1BL.1RS translocations. AU - Luo,Jie, AU - Liao,Ruiying, AU - Duan,Yanling, AU - Fu,Shulan, AU - Tang,Zongxiang, Y1 - 2022/04/25/ PY - 2022/01/29/received PY - 2022/04/15/accepted PY - 2022/4/26/entrez PY - 2022/4/27/pubmed PY - 2022/4/28/medline KW - 1BL.1RS KW - 1RS polymorphism KW - Rye KW - Tandem repeat KW - Wheat SP - 212 EP - 212 JF - BMC plant biology JO - BMC Plant Biol VL - 22 IS - 1 N2 - BACKGROUND: The wheat-rye 1BL.1RS translocations have played an important role in common wheat breeding programs. Subtelomeric tandem repeats have been often used to investigate polymorphisms of 1RS arms, but further research about their organizations on the 1RS chromosome is needed. RESULTS: 162 1RS arms from a wild rye species (Secale strictum) and six cultivated rye accessions (Secale cereale L.) (81 plants), 102 1BL.1RS and one 1AL.1RS translocations were investigated using oligo probes Oligo-TaiI, Oligo-pSc119.2-1, Oligo-pTa71A-2, Oligo-pSc200 and Oligo-pSc250, which were derived from tandem repeats TaiI, pSc119.2, pTa71, pSc200 and pSc250, respectively. The variations of 1RS arms were revealed by signal intensity of probes Oligo-pSc119.2-1, Oligo-pTa71A-2, Oligo-pSc200 and Oligo-pSc250. Proliferation of rDNA sequences on the 1RS chromosomes was observed. According to the presence of probe signals, 34, 127 and 144 of the 162 1RS arms contained TaiI, pSc200 and pSc250, respectively, and all of them contained pSc119.2 and pTa71. Most of the 1RS arms in rye contained three kinds of subtelomeric tandem repeats, the combination of pSc119.2, pSc200 and pSc250 was most common, and only eight of them contained TaiI, pSc119.2, pSc200 and pSc250. All of the 1RS arms in 1BL.1RS and 1AL.1RS translocations contained pSc119.2, pTa71, pSc200 and pSc250, but the presence of the TaiI family was not observed. CONCLUSION: New organizations of subtelomeric tandem repeats on 1RS were found, and they reflected new genetic variations of 1RS arms. These 1RS arms might contain abundant allelic diversity for agricultural traits. The narrow genetic base of 1RS arms in 1BL.1RS and 1AL.1RS translocations currently used in agriculture is seriously restricting their use in wheat breeding programs. This research has found new 1RS sources for the future restructuring of 1BL.1RS translocations. The allelic variations of these 1RS arms should be studied more intensely as they may enrich the genetic diversity of 1BL.1RS translocations. SN - 1471-2229 UR - https://www.unboundmedicine.com/medline/citation/35468732/Variations_of_subtelomeric_tandem_repeats_and_rDNA_on_chromosome_1RS_arms_in_the_genus_Secale_and_1BL_1RS_translocations_ DB - PRIME DP - Unbound Medicine ER -