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Characterization of the virE locus of Agrobacterium tumefaciens plasmid pTiC58.
J Bacteriol 1987; 169(4):1529-36JB

Abstract

The virE locus that is responsible for the efficiency of infection by Agrobacterium tumefaciens (T. Hirooka and C. Kado, J. Bacteriol. 168:237-243, 1986) is located next to the right boundary of the virulence (Vir) region of the nopaline plasmid pTiC58. This locus is very similar to the virE locus of octopine type Ti plasmids on the basis of nucleotide and amino acid sequence comparisons as well as genetic complementation analyses. The nucleotide sequence of virE revealed three open reading frames, arranged as an operon, with a potential coding capacity for proteins of 9, 7.1, and 63.5 kilodaltons. The promoter region of virE was analyzed by using gene fusions to promoterless cat and lux genes. Two different promoters were detected, one which operates in A. tumefaciens and one which operates in Escherichia coli. virE is transcribed from left to right toward the T region. In A. tumefaciens, the expression of virE was induced by acetosyringone and required the presence of pTiC58.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

3549694

Citation

Hirooka, T, et al. "Characterization of the virE Locus of Agrobacterium Tumefaciens Plasmid PTiC58." Journal of Bacteriology, vol. 169, no. 4, 1987, pp. 1529-36.
Hirooka T, Rogowsky PM, Kado CI. Characterization of the virE locus of Agrobacterium tumefaciens plasmid pTiC58. J Bacteriol. 1987;169(4):1529-36.
Hirooka, T., Rogowsky, P. M., & Kado, C. I. (1987). Characterization of the virE locus of Agrobacterium tumefaciens plasmid pTiC58. Journal of Bacteriology, 169(4), pp. 1529-36.
Hirooka T, Rogowsky PM, Kado CI. Characterization of the virE Locus of Agrobacterium Tumefaciens Plasmid PTiC58. J Bacteriol. 1987;169(4):1529-36. PubMed PMID: 3549694.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of the virE locus of Agrobacterium tumefaciens plasmid pTiC58. AU - Hirooka,T, AU - Rogowsky,P M, AU - Kado,C I, PY - 1987/4/1/pubmed PY - 1987/4/1/medline PY - 1987/4/1/entrez SP - 1529 EP - 36 JF - Journal of bacteriology JO - J. Bacteriol. VL - 169 IS - 4 N2 - The virE locus that is responsible for the efficiency of infection by Agrobacterium tumefaciens (T. Hirooka and C. Kado, J. Bacteriol. 168:237-243, 1986) is located next to the right boundary of the virulence (Vir) region of the nopaline plasmid pTiC58. This locus is very similar to the virE locus of octopine type Ti plasmids on the basis of nucleotide and amino acid sequence comparisons as well as genetic complementation analyses. The nucleotide sequence of virE revealed three open reading frames, arranged as an operon, with a potential coding capacity for proteins of 9, 7.1, and 63.5 kilodaltons. The promoter region of virE was analyzed by using gene fusions to promoterless cat and lux genes. Two different promoters were detected, one which operates in A. tumefaciens and one which operates in Escherichia coli. virE is transcribed from left to right toward the T region. In A. tumefaciens, the expression of virE was induced by acetosyringone and required the presence of pTiC58. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/3549694/Characterization_of_the_virE_locus_of_Agrobacterium_tumefaciens_plasmid_pTiC58_ L2 - http://jb.asm.org/cgi/pmidlookup?view=long&pmid=3549694 DB - PRIME DP - Unbound Medicine ER -