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Histological and ultrastructural localization of antigen B in the metacestode of Taenia solium.
J Parasitol 1987; 73(1):121-9JP

Abstract

The morphological localization of antigen B (AgB) in the tissues of the Taenia solium metacestode was studied by immunological and biochemical methods. Indirect immunofluorescence carried out on vibratome sections showed that AgB is widely distributed throughout the tissue. A more intense fluorescence was observed in the tegumentary cytons of the bladder wall and in the lumen of the spiral canal of the invaginated scolex. Ultrastructural analysis of larvae washed in PBS after dissection from meat and then incubated with rabbit antibodies against AgB, followed by peroxidase-labeled goat anti-rabbit IgG, did not exhibit electron-dense material on the external surface. Larvae fixed in glutaraldehyde immediately after dissection and exposed to the immunoperoxidase reagents did exhibit electron-dense material on microtriches, indicating that AgB is only loosely bound to the external surface. Crude extracts of surface-radioiodinated cysticerci analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) contained no labeled proteins with the molecular weight of AgB. Autoradiography of the immunoelectrophoretograms in which the crude extract was confronted with antibodies to AgB demonstrated that this antigen was not labeled, and therefore is not exposed on the tegumentary surface. The results suggest that AgB is synthesized by the tegumentary cytons of the parasite and secreted through the tegumental membrane into the host tissues and the lumen of the spiral canal.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

3553514

Citation

Laclette, J P., et al. "Histological and Ultrastructural Localization of Antigen B in the Metacestode of Taenia Solium." The Journal of Parasitology, vol. 73, no. 1, 1987, pp. 121-9.
Laclette JP, Merchant MT, Willms K. Histological and ultrastructural localization of antigen B in the metacestode of Taenia solium. J Parasitol. 1987;73(1):121-9.
Laclette, J. P., Merchant, M. T., & Willms, K. (1987). Histological and ultrastructural localization of antigen B in the metacestode of Taenia solium. The Journal of Parasitology, 73(1), pp. 121-9.
Laclette JP, Merchant MT, Willms K. Histological and Ultrastructural Localization of Antigen B in the Metacestode of Taenia Solium. J Parasitol. 1987;73(1):121-9. PubMed PMID: 3553514.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Histological and ultrastructural localization of antigen B in the metacestode of Taenia solium. AU - Laclette,J P, AU - Merchant,M T, AU - Willms,K, PY - 1987/2/1/pubmed PY - 1987/2/1/medline PY - 1987/2/1/entrez SP - 121 EP - 9 JF - The Journal of parasitology JO - J. Parasitol. VL - 73 IS - 1 N2 - The morphological localization of antigen B (AgB) in the tissues of the Taenia solium metacestode was studied by immunological and biochemical methods. Indirect immunofluorescence carried out on vibratome sections showed that AgB is widely distributed throughout the tissue. A more intense fluorescence was observed in the tegumentary cytons of the bladder wall and in the lumen of the spiral canal of the invaginated scolex. Ultrastructural analysis of larvae washed in PBS after dissection from meat and then incubated with rabbit antibodies against AgB, followed by peroxidase-labeled goat anti-rabbit IgG, did not exhibit electron-dense material on the external surface. Larvae fixed in glutaraldehyde immediately after dissection and exposed to the immunoperoxidase reagents did exhibit electron-dense material on microtriches, indicating that AgB is only loosely bound to the external surface. Crude extracts of surface-radioiodinated cysticerci analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) contained no labeled proteins with the molecular weight of AgB. Autoradiography of the immunoelectrophoretograms in which the crude extract was confronted with antibodies to AgB demonstrated that this antigen was not labeled, and therefore is not exposed on the tegumentary surface. The results suggest that AgB is synthesized by the tegumentary cytons of the parasite and secreted through the tegumental membrane into the host tissues and the lumen of the spiral canal. SN - 0022-3395 UR - https://www.unboundmedicine.com/medline/citation/3553514/Histological_and_ultrastructural_localization_of_antigen_B_in_the_metacestode_of_Taenia_solium DB - PRIME DP - Unbound Medicine ER -