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High-resolution detection of quantitative trait loci for seven important yield-related traits in wheat (Triticum aestivum L.) using a high-density SLAF-seq genetic map.
BMC Genom Data. 2022 May 13; 23(1):37.BG

Abstract

BACKGROUND

Yield-related traits including thousand grain weight (TGW), grain number per spike (GNS), grain width (GW), grain length (GL), plant height (PH), spike length (SL), and spikelet number per spike (SNS) are greatly associated with grain yield of wheat (Triticum aestivum L.). To detect quantitative trait loci (QTL) associated with them, 193 recombinant inbred lines derived from two elite winter wheat varieties Chuanmai42 and Chuanmai39 were employed to perform QTL mapping in six/eight environments.

RESULTS

A total of 30 QTLs on chromosomes 1A, 1B, 1D, 2A, 2B, 2D, 3A, 4A, 5A, 5B, 6A, 6D, 7A, 7B and 7D were identified. Among them, six major QTLs QTgw.cib-6A.1, QTgw.cib-6A.2, QGw.cib-6A, QGl.cib-3A, QGl.cib-6A, and QSl.cib-2D explaining 5.96-23.75% of the phenotypic variance were detected in multi-environments and showed strong and stable effects on corresponding traits. Three QTL clusters on chromosomes 2D and 6A containing 10 QTLs were also detected, which showed significant pleiotropic effects on multiple traits. Additionally, three Kompetitive Allele Specific PCR (KASP) markers linked with five of these major QTLs were developed. Candidate genes of QTgw.cib-6A.1/QGl.cib-6A and QGl.cib-3A were analyzed based on the spatiotemporal expression patterns, gene annotation, and orthologous search.

CONCLUSIONS

Six major QTLs for TGW, GL, GW and SL were detected. Three KASP markers linked with five of these major QTLs were developed. These QTLs and KASP markers will be useful for elucidating the genetic architecture of grain yield and developing new wheat varieties with high and stable yield in wheat.

Authors+Show Affiliations

Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China. Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, China. State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Chengdu, 611130, China.Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China.Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China.Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China.Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China.Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China.Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China.Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China.Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China.Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China.Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China.Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China.Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 610066, Sichuan, China.Crop Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu, 610066, Sichuan, China.State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Chengdu, 611130, China.Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China.Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China.Triticeae Research Institute, Sichuan Agricultural University, Chengdu, 611130, China. State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Chengdu, 611130, China.Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu, 610041, China. denggb@cib.ac.cn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

35562674

Citation

Li, Tao, et al. "High-resolution Detection of Quantitative Trait Loci for Seven Important Yield-related Traits in Wheat (Triticum Aestivum L.) Using a High-density SLAF-seq Genetic Map." BMC Genomic Data, vol. 23, no. 1, 2022, p. 37.
Li T, Li Q, Wang J, et al. High-resolution detection of quantitative trait loci for seven important yield-related traits in wheat (Triticum aestivum L.) using a high-density SLAF-seq genetic map. BMC Genom Data. 2022;23(1):37.
Li, T., Li, Q., Wang, J., Yang, Z., Tang, Y., Su, Y., Zhang, J., Qiu, X., Pu, X., Pan, Z., Zhang, H., Liang, J., Liu, Z., Li, J., Yan, W., Yu, M., Long, H., Wei, Y., & Deng, G. (2022). High-resolution detection of quantitative trait loci for seven important yield-related traits in wheat (Triticum aestivum L.) using a high-density SLAF-seq genetic map. BMC Genomic Data, 23(1), 37. https://doi.org/10.1186/s12863-022-01050-0
Li T, et al. High-resolution Detection of Quantitative Trait Loci for Seven Important Yield-related Traits in Wheat (Triticum Aestivum L.) Using a High-density SLAF-seq Genetic Map. BMC Genom Data. 2022 May 13;23(1):37. PubMed PMID: 35562674.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High-resolution detection of quantitative trait loci for seven important yield-related traits in wheat (Triticum aestivum L.) using a high-density SLAF-seq genetic map. AU - Li,Tao, AU - Li,Qiao, AU - Wang,Jinhui, AU - Yang,Zhao, AU - Tang,Yanyan, AU - Su,Yan, AU - Zhang,Juanyu, AU - Qiu,Xvebing, AU - Pu,Xi, AU - Pan,Zhifen, AU - Zhang,Haili, AU - Liang,Junjun, AU - Liu,Zehou, AU - Li,Jun, AU - Yan,Wuyun, AU - Yu,Maoqun, AU - Long,Hai, AU - Wei,Yuming, AU - Deng,Guangbing, Y1 - 2022/05/13/ PY - 2021/08/05/received PY - 2022/04/06/accepted PY - 2022/5/13/entrez PY - 2022/5/14/pubmed PY - 2022/5/18/medline KW - Linkage analysis KW - Specific-locus amplified fragment (SLAF) KW - Wheat KW - Yield KW - Yield-related traits SP - 37 EP - 37 JF - BMC genomic data JO - BMC Genom Data VL - 23 IS - 1 N2 - BACKGROUND: Yield-related traits including thousand grain weight (TGW), grain number per spike (GNS), grain width (GW), grain length (GL), plant height (PH), spike length (SL), and spikelet number per spike (SNS) are greatly associated with grain yield of wheat (Triticum aestivum L.). To detect quantitative trait loci (QTL) associated with them, 193 recombinant inbred lines derived from two elite winter wheat varieties Chuanmai42 and Chuanmai39 were employed to perform QTL mapping in six/eight environments. RESULTS: A total of 30 QTLs on chromosomes 1A, 1B, 1D, 2A, 2B, 2D, 3A, 4A, 5A, 5B, 6A, 6D, 7A, 7B and 7D were identified. Among them, six major QTLs QTgw.cib-6A.1, QTgw.cib-6A.2, QGw.cib-6A, QGl.cib-3A, QGl.cib-6A, and QSl.cib-2D explaining 5.96-23.75% of the phenotypic variance were detected in multi-environments and showed strong and stable effects on corresponding traits. Three QTL clusters on chromosomes 2D and 6A containing 10 QTLs were also detected, which showed significant pleiotropic effects on multiple traits. Additionally, three Kompetitive Allele Specific PCR (KASP) markers linked with five of these major QTLs were developed. Candidate genes of QTgw.cib-6A.1/QGl.cib-6A and QGl.cib-3A were analyzed based on the spatiotemporal expression patterns, gene annotation, and orthologous search. CONCLUSIONS: Six major QTLs for TGW, GL, GW and SL were detected. Three KASP markers linked with five of these major QTLs were developed. These QTLs and KASP markers will be useful for elucidating the genetic architecture of grain yield and developing new wheat varieties with high and stable yield in wheat. SN - 2730-6844 UR - https://www.unboundmedicine.com/medline/citation/35562674/High_resolution_detection_of_quantitative_trait_loci_for_seven_important_yield_related_traits_in_wheat__Triticum_aestivum_L___using_a_high_density_SLAF_seq_genetic_map_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/35562674/ DB - PRIME DP - Unbound Medicine ER -