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Enantioseparation of basic drugs by reverse phase high-performance liquid chromatography system using carboxymethyl-β-cyclodextrin as chiral mobile phase additive.
Chirality. 2022 08; 34(8):1128-1139.C

Abstract

A rapid and efficient method was developed for enantioseparation of basic drugs, using carboxymethyl-β-cyclodextrin (CM-β-CD) as chiral mobile phase additive, rather than involving costly chiral column in high-performance liquid chromatography (HPLC) system. Four of the six basic drug enantiomers investigated were successfully separated. The highest resolution reaches 2.15 for threo-(1S,2S)-2-amino-l-p-nitrophenyl-1,3-propanediol. The effects of the organic modifier, pH value, concentration of chiral additive, column temperature, and flow rate of mobile phase on the enantioseparation of analytes were researched. The apparent formation constants of inclusion and the thermodynamic parameters were evaluated to explain the mechanism of chiral recognition.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Ao Q
School of Chemical Engineering, East China University of Science and Technology, Shanghai, China.
Zhao H
School of Chemical Engineering, East China University of Science and Technology, Shanghai, China.
Tong T
School of Chemical Engineering, East China University of Science and Technology, Shanghai, China.
Peng Y
School of Chemical Engineering, East China University of Science and Technology, Shanghai, China.
He Z
School of Chemical Engineering, East China University of Science and Technology, Shanghai, China.

MeSH

Chromatography, High Pressure LiquidChromatography, Reverse-PhaseStereoisomerismbeta-Cyclodextrins

Pub Type(s)

Journal Article

Language

eng

PubMed ID

35574700

Citation

Ao, Qiong, et al. "Enantioseparation of Basic Drugs By Reverse Phase High-performance Liquid Chromatography System Using Carboxymethyl-β-cyclodextrin as Chiral Mobile Phase Additive." Chirality, vol. 34, no. 8, 2022, pp. 1128-1139.
Ao Q, Zhao H, Tong T, et al. Enantioseparation of basic drugs by reverse phase high-performance liquid chromatography system using carboxymethyl-β-cyclodextrin as chiral mobile phase additive. Chirality. 2022;34(8):1128-1139.
Ao, Q., Zhao, H., Tong, T., Peng, Y., & He, Z. (2022). Enantioseparation of basic drugs by reverse phase high-performance liquid chromatography system using carboxymethyl-β-cyclodextrin as chiral mobile phase additive. Chirality, 34(8), 1128-1139. https://doi.org/10.1002/chir.23470
Ao Q, et al. Enantioseparation of Basic Drugs By Reverse Phase High-performance Liquid Chromatography System Using Carboxymethyl-β-cyclodextrin as Chiral Mobile Phase Additive. Chirality. 2022;34(8):1128-1139. PubMed PMID: 35574700.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enantioseparation of basic drugs by reverse phase high-performance liquid chromatography system using carboxymethyl-β-cyclodextrin as chiral mobile phase additive. AU - Ao,Qiong, AU - Zhao,Hongliang, AU - Tong,Tianzhong, AU - Peng,Yangfeng, AU - He,Zhaoyang, Y1 - 2022/05/16/ PY - 2022/04/26/revised PY - 2022/04/11/received PY - 2022/04/28/accepted PY - 2022/5/17/pubmed PY - 2022/7/15/medline PY - 2022/5/16/entrez KW - HPLC KW - basic drug KW - carboxymethyl-β-cyclodextrin KW - chiral mobile phase additive KW - enantioseparation SP - 1128 EP - 1139 JF - Chirality JO - Chirality VL - 34 IS - 8 N2 - A rapid and efficient method was developed for enantioseparation of basic drugs, using carboxymethyl-β-cyclodextrin (CM-β-CD) as chiral mobile phase additive, rather than involving costly chiral column in high-performance liquid chromatography (HPLC) system. Four of the six basic drug enantiomers investigated were successfully separated. The highest resolution reaches 2.15 for threo-(1S,2S)-2-amino-l-p-nitrophenyl-1,3-propanediol. The effects of the organic modifier, pH value, concentration of chiral additive, column temperature, and flow rate of mobile phase on the enantioseparation of analytes were researched. The apparent formation constants of inclusion and the thermodynamic parameters were evaluated to explain the mechanism of chiral recognition. SN - 1520-636X UR - https://www.unboundmedicine.com/medline/citation/35574700/Enantioseparation_of_basic_drugs_by_reverse_phase_high_performance_liquid_chromatography_system_using_carboxymethyl_β_cyclodextrin_as_chiral_mobile_phase_additive_ DB - PRIME DP - Unbound Medicine ER -