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Deciphering resistance to Zymoseptoria tritici in the Tunisian durum wheat landrace accession 'Agili39'.
BMC Genomics. 2022 May 17; 23(1):372.BG

Abstract

BACKGROUND

Septoria tritici blotch (STB), caused by Zymoseptoria tritici (Z. tritici), is an important biotic threat to durum wheat in the entire Mediterranean Basin. Although most durum wheat cultivars are susceptible to Z. tritici, research in STB resistance in durum wheat has been limited.

RESULTS

In our study, we have identified resistance to a wide array of Z. tritici isolates in the Tunisian durum wheat landrace accession 'Agili39'. Subsequently, a recombinant inbred population was developed and tested under greenhouse conditions at the seedling stage with eight Z. tritici isolates and for five years under field conditions with three Z. tritici isolates. Mapping of quantitative trait loci (QTL) resulted in the identification of two major QTL on chromosome 2B designated as Qstb2B_1 and Qstb2B_2. The Qstb2B_1 QTL was mapped at the seedling and the adult plant stage (highest LOD 33.9, explained variance 61.6%), conferring an effective resistance against five Z. tritici isolates. The Qstb2B_2 conferred adult plant resistance (highest LOD 32.9, explained variance 42%) and has been effective at the field trials against two Z. tritici isolates. The physical positions of the flanking markers linked to Qstb2B_1 and Qstb2B_2 indicate that these two QTL are 5 Mb apart. In addition, we identified two minor QTL on chromosomes 1A (Qstb1A) and chromosome 7A (Qstb7A) (highest LODs 4.6 and 4.0, and explained variances of 16% and 9%, respectively) that were specific to three and one Z. tritici isolates, respectively. All identified QTL were derived from the landrace accession Agili39 that represents a valuable source for STB resistance in durum wheat.

CONCLUSION

This study demonstrates that Z. tritici resistance in the 'Agili39' landrace accession is controlled by two minor and two major QTL acting in an additive mode. We also provide evidence that the broad efficacy of the resistance to STB in 'Agili 39' is due to a natural pyramiding of these QTL. A sustainable use of this Z. tritici resistance source and a positive selection of the linked markers to the identified QTL will greatly support effective breeding for Z. tritici resistance in durum wheat.

Authors+Show Affiliations

Laboratory of Bioaggressors and Integrated Protection in Agriculture (BPIA), National Institute of Agronomy of Tunisia (INAT), 43 Avenue Charles Nicolle, 1082 El Mahrajène, Tunis, Tunisia. Present Address Field Crops Laboratory, Regional Field Crops Research Center of Beja (CRRGC), P.O. Box 9000, Beja, Tunisia.Bio-Interaction and Plant Health, Wageningen University and Research, PO Box 16, 6700AA, Wageningen, The Netherlands. The Graduate School 'Experimental Plant Sciences' (EPS), Wageningen Campus, 6708 PB, Wageningen, The Netherlands. Present Address Center for Desert Agriculture, Biological and Environmental Science and Engineering Division, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia.Laboratory of Bioaggressors and Integrated Protection in Agriculture (BPIA), National Institute of Agronomy of Tunisia (INAT), 43 Avenue Charles Nicolle, 1082 El Mahrajène, Tunis, Tunisia. Present address Higher Institute of Agronomy of Chott Meriam (ISA-CM), 4042, Sousse, Tunisia.International Maize and Wheat Improvement Center (CIMMYT), Apdo. Postal 6‑641, 06600, Mexico, D.F., Mexico.International Maize and Wheat Improvement Center (CIMMYT), Apdo. Postal 6‑641, 06600, Mexico, D.F., Mexico.Plant Breeding, Wageningen University and Research, P.O. Box 386, 6700 AJ, Wageningen, The Netherlands.Institute of Plant Breeding, Genetics and Genomics, Department of Plant Pathology and Institute of Plant Breeding, University of Georgia, Griffin, GA, 30223, USA. Present Address United States Department of Agriculture USDA, Crop Genetics and Breeding Research Unit, Tifton, GA, USA.Laboratory of Bioaggressors and Integrated Protection in Agriculture (BPIA), National Institute of Agronomy of Tunisia (INAT), 43 Avenue Charles Nicolle, 1082 El Mahrajène, Tunis, Tunisia. Institute of Plant Breeding, Genetics and Genomics, Department of Plant Pathology and Institute of Plant Breeding, University of Georgia, Griffin, GA, 30223, USA.CRP-Wheat Septoria Phenotyping Platform (CIMMYT-IRESA), Regional Field Crops Research Center of Beja (CRRGC), BP 350, 9000, Beja, Tunisia.Plant Breeding, Wageningen University and Research, P.O. Box 386, 6700 AJ, Wageningen, The Netherlands.Bio-Interaction and Plant Health, Wageningen University and Research, PO Box 16, 6700AA, Wageningen, The Netherlands. Laboratory of Phytopathology, Wageningen University and Research, PO box 16, 6700AA, Wageningen, The Netherlands.Laboratory of Bioaggressors and Integrated Protection in Agriculture (BPIA), National Institute of Agronomy of Tunisia (INAT), 43 Avenue Charles Nicolle, 1082 El Mahrajène, Tunis, Tunisia. hamza.sonia@inat.agrinet.tn.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

35581550

Citation

Ferjaoui, Sahbi, et al. "Deciphering Resistance to Zymoseptoria Tritici in the Tunisian Durum Wheat Landrace Accession 'Agili39'." BMC Genomics, vol. 23, no. 1, 2022, p. 372.
Ferjaoui S, Aouini L, Slimane RB, et al. Deciphering resistance to Zymoseptoria tritici in the Tunisian durum wheat landrace accession 'Agili39'. BMC Genomics. 2022;23(1):372.
Ferjaoui, S., Aouini, L., Slimane, R. B., Ammar, K., Dreisigacker, S., Schouten, H. J., Sapkota, S., Bahri, B. A., Ben M'Barek, S., Visser, R. G. F., Kema, G. H. J., & Hamza, S. (2022). Deciphering resistance to Zymoseptoria tritici in the Tunisian durum wheat landrace accession 'Agili39'. BMC Genomics, 23(1), 372. https://doi.org/10.1186/s12864-022-08560-2
Ferjaoui S, et al. Deciphering Resistance to Zymoseptoria Tritici in the Tunisian Durum Wheat Landrace Accession 'Agili39'. BMC Genomics. 2022 May 17;23(1):372. PubMed PMID: 35581550.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Deciphering resistance to Zymoseptoria tritici in the Tunisian durum wheat landrace accession 'Agili39'. AU - Ferjaoui,Sahbi, AU - Aouini,Lamia, AU - Slimane,Rim B, AU - Ammar,Karim, AU - Dreisigacker,Suzanne, AU - Schouten,Henk J, AU - Sapkota,Suraj, AU - Bahri,Bochra A, AU - Ben M'Barek,Sarrah, AU - Visser,Richard G F, AU - Kema,Gert H J, AU - Hamza,Sonia, Y1 - 2022/05/17/ PY - 2021/09/20/received PY - 2022/04/14/accepted PY - 2022/5/17/entrez PY - 2022/5/18/pubmed PY - 2022/5/20/medline KW - Durum wheat KW - Gene efficacy KW - Gene pyramiding KW - Landraces KW - Mycosphaerella graminicola quantitative trait loci (QTL) KW - QTL epistasis KW - Zymoseptoria tritici SP - 372 EP - 372 JF - BMC genomics JO - BMC Genomics VL - 23 IS - 1 N2 - BACKGROUND: Septoria tritici blotch (STB), caused by Zymoseptoria tritici (Z. tritici), is an important biotic threat to durum wheat in the entire Mediterranean Basin. Although most durum wheat cultivars are susceptible to Z. tritici, research in STB resistance in durum wheat has been limited. RESULTS: In our study, we have identified resistance to a wide array of Z. tritici isolates in the Tunisian durum wheat landrace accession 'Agili39'. Subsequently, a recombinant inbred population was developed and tested under greenhouse conditions at the seedling stage with eight Z. tritici isolates and for five years under field conditions with three Z. tritici isolates. Mapping of quantitative trait loci (QTL) resulted in the identification of two major QTL on chromosome 2B designated as Qstb2B_1 and Qstb2B_2. The Qstb2B_1 QTL was mapped at the seedling and the adult plant stage (highest LOD 33.9, explained variance 61.6%), conferring an effective resistance against five Z. tritici isolates. The Qstb2B_2 conferred adult plant resistance (highest LOD 32.9, explained variance 42%) and has been effective at the field trials against two Z. tritici isolates. The physical positions of the flanking markers linked to Qstb2B_1 and Qstb2B_2 indicate that these two QTL are 5 Mb apart. In addition, we identified two minor QTL on chromosomes 1A (Qstb1A) and chromosome 7A (Qstb7A) (highest LODs 4.6 and 4.0, and explained variances of 16% and 9%, respectively) that were specific to three and one Z. tritici isolates, respectively. All identified QTL were derived from the landrace accession Agili39 that represents a valuable source for STB resistance in durum wheat. CONCLUSION: This study demonstrates that Z. tritici resistance in the 'Agili39' landrace accession is controlled by two minor and two major QTL acting in an additive mode. We also provide evidence that the broad efficacy of the resistance to STB in 'Agili 39' is due to a natural pyramiding of these QTL. A sustainable use of this Z. tritici resistance source and a positive selection of the linked markers to the identified QTL will greatly support effective breeding for Z. tritici resistance in durum wheat. SN - 1471-2164 UR - https://www.unboundmedicine.com/medline/citation/35581550/Deciphering_resistance_to_Zymoseptoria_tritici_in_the_Tunisian_durum_wheat_landrace_accession_'Agili39'_ L2 - https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-022-08560-2 DB - PRIME DP - Unbound Medicine ER -