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Selected fungi of the genus Lactarius - screening of antioxidant capacity, antimicrobial activity, and genotoxicity.
J Toxicol Environ Health A. 2022 Sep 02; 85(17):699-714.JT

Abstract

It is well-known that mushrooms of the genus Lactarius constitute a natural food resource providing health benefits as a nutritient. This genus contains 4 mushrooms identified as L. deliciosus, L. volemus, L. sanguifluus, L. semisanguifluus and L. piperatus which were collected in Serbia. The aim of this study aimed was to identify and characterize the content of phenolic compounds and examine the antioxidant potential of 5 wild edible mushrooms. L. sanguifluus contained the highest content of total phenol compounds (75.25 mg gallic acid equivalents (GAE) per g dry extract weight (GAE/g DE) and exhibited the greatest antioxidant activity through the ability to remove radicals as evidenced by ABTS assay (8.99 mg of trolox equivalents (TE) per g dry extract weight (mg TE/g DE); total reducing power (TRP) assay mg ascorbic acid equivalents per mg of dry extract weight (0.42 mg AAE/g DE) and CUPRAC (14.23 mg TE /g DE). L. deliciosus methanolic extract produced greatest scavenging of the DPPH radical (46%). The methanol mushroom extracts were screened for in vitro antimicrobial activity against a panel of pathogenic bacterial strains using the microdilution method. Of all the extracts tested, L. sanguifluus extract showed the best antibacterial properties. The cytokinesis block micronucleus assay results for the examined mushrooms demonstrated that extracts at a concentration of 3 μg/ml decreased the number of micronuclei (MN) in the range of 19-49% which is significant bearing in mind that radioprotectant amifostine reduced the frequency of MN by only 16.3%. Data thus demonstrate that the 5 wild edible mushrooms of genus Lactarius contain constituents that are beneficial not only as nutrients but also have the potential as antioxidants, antibacterial and antigenotoxic properties.

Authors+Show Affiliations

Nuclear Facilities of Serbia, Vinča, Belgrade, Serbia.Department of Chemistry, Faculty of Science and Mathematics, University of Niš, Niš, Serbia.Department of Chemistry, Faculty of Science and Mathematics, University of Niš, Niš, Serbia.Department of Pharmacy, Faculty of Medicine, University of Niš, Niš, Serbia.Department of Chemistry, Faculty of Science and Mathematics, University of Niš, Niš, Serbia.Department of Chemistry, Faculty of Science and Mathematics, University of Niš, Niš, Serbia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

35591784

Citation

Stanković, Miroslava, et al. "Selected Fungi of the Genus Lactarius - Screening of Antioxidant Capacity, Antimicrobial Activity, and Genotoxicity." Journal of Toxicology and Environmental Health. Part A, vol. 85, no. 17, 2022, pp. 699-714.
Stanković M, Mitić V, Stankov Jovanović V, et al. Selected fungi of the genus Lactarius - screening of antioxidant capacity, antimicrobial activity, and genotoxicity. J Toxicol Environ Health A. 2022;85(17):699-714.
Stanković, M., Mitić, V., Stankov Jovanović, V., Dimitrijević, M., Nikolić, J., & Stojanović, G. (2022). Selected fungi of the genus Lactarius - screening of antioxidant capacity, antimicrobial activity, and genotoxicity. Journal of Toxicology and Environmental Health. Part A, 85(17), 699-714. https://doi.org/10.1080/15287394.2022.2075502
Stanković M, et al. Selected Fungi of the Genus Lactarius - Screening of Antioxidant Capacity, Antimicrobial Activity, and Genotoxicity. J Toxicol Environ Health A. 2022 Sep 2;85(17):699-714. PubMed PMID: 35591784.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Selected fungi of the genus Lactarius - screening of antioxidant capacity, antimicrobial activity, and genotoxicity. AU - Stanković,Miroslava, AU - Mitić,Violeta, AU - Stankov Jovanović,Vesna, AU - Dimitrijević,Marija, AU - Nikolić,Jelena, AU - Stojanović,Gordana, Y1 - 2022/05/19/ PY - 2022/5/21/pubmed PY - 2022/5/21/medline PY - 2022/5/20/entrez KW - Lactarius spp. KW - antimicrobial activity KW - antioxidant capacity KW - chemometry KW - genotoxicity SP - 699 EP - 714 JF - Journal of toxicology and environmental health. Part A JO - J Toxicol Environ Health A VL - 85 IS - 17 N2 - It is well-known that mushrooms of the genus Lactarius constitute a natural food resource providing health benefits as a nutritient. This genus contains 4 mushrooms identified as L. deliciosus, L. volemus, L. sanguifluus, L. semisanguifluus and L. piperatus which were collected in Serbia. The aim of this study aimed was to identify and characterize the content of phenolic compounds and examine the antioxidant potential of 5 wild edible mushrooms. L. sanguifluus contained the highest content of total phenol compounds (75.25 mg gallic acid equivalents (GAE) per g dry extract weight (GAE/g DE) and exhibited the greatest antioxidant activity through the ability to remove radicals as evidenced by ABTS assay (8.99 mg of trolox equivalents (TE) per g dry extract weight (mg TE/g DE); total reducing power (TRP) assay mg ascorbic acid equivalents per mg of dry extract weight (0.42 mg AAE/g DE) and CUPRAC (14.23 mg TE /g DE). L. deliciosus methanolic extract produced greatest scavenging of the DPPH radical (46%). The methanol mushroom extracts were screened for in vitro antimicrobial activity against a panel of pathogenic bacterial strains using the microdilution method. Of all the extracts tested, L. sanguifluus extract showed the best antibacterial properties. The cytokinesis block micronucleus assay results for the examined mushrooms demonstrated that extracts at a concentration of 3 μg/ml decreased the number of micronuclei (MN) in the range of 19-49% which is significant bearing in mind that radioprotectant amifostine reduced the frequency of MN by only 16.3%. Data thus demonstrate that the 5 wild edible mushrooms of genus Lactarius contain constituents that are beneficial not only as nutrients but also have the potential as antioxidants, antibacterial and antigenotoxic properties. SN - 1528-7394 UR - https://www.unboundmedicine.com/medline/citation/35591784/Selected_fungi_of_the_genus_Lactarius___screening_of_antioxidant_capacity_antimicrobial_activity_and_genotoxicity_ L2 - https://www.tandfonline.com/doi/full/10.1080/15287394.2022.2075502 DB - PRIME DP - Unbound Medicine ER -