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Monoclonal antibody ELISA to quantitate wheat gliadin contamination of gluten-free foods.
J Immunol Methods. 1987 Apr 02; 98(1):123-7.JI

Abstract

A sensitive reproducible monoclonal antibody-based sandwich ELISA has been developed to measure the gliadin content of foods. Using the assay we have confirmed that nominally gluten-free products based on wheat starch contain trace amounts of gliadin. The level of gliadin contamination in these foods is sufficient to cause relapse of coeliac disease in specific patients. We have also examined the cross-reactivity of different cereal proteins in the assay and found good correlation with their known toxicity to patients with coeliac disease.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

3559211

Citation

Freedman, A R., et al. "Monoclonal Antibody ELISA to Quantitate Wheat Gliadin Contamination of Gluten-free Foods." Journal of Immunological Methods, vol. 98, no. 1, 1987, pp. 123-7.
Freedman AR, Galfrè G, Gal E, et al. Monoclonal antibody ELISA to quantitate wheat gliadin contamination of gluten-free foods. J Immunol Methods. 1987;98(1):123-7.
Freedman, A. R., Galfrè, G., Gal, E., Ellis, H. J., & Ciclitira, P. J. (1987). Monoclonal antibody ELISA to quantitate wheat gliadin contamination of gluten-free foods. Journal of Immunological Methods, 98(1), 123-7.
Freedman AR, et al. Monoclonal Antibody ELISA to Quantitate Wheat Gliadin Contamination of Gluten-free Foods. J Immunol Methods. 1987 Apr 2;98(1):123-7. PubMed PMID: 3559211.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Monoclonal antibody ELISA to quantitate wheat gliadin contamination of gluten-free foods. AU - Freedman,A R, AU - Galfrè,G, AU - Gal,E, AU - Ellis,H J, AU - Ciclitira,P J, PY - 1987/4/2/pubmed PY - 1987/4/2/medline PY - 1987/4/2/entrez SP - 123 EP - 7 JF - Journal of immunological methods JO - J. Immunol. Methods VL - 98 IS - 1 N2 - A sensitive reproducible monoclonal antibody-based sandwich ELISA has been developed to measure the gliadin content of foods. Using the assay we have confirmed that nominally gluten-free products based on wheat starch contain trace amounts of gliadin. The level of gliadin contamination in these foods is sufficient to cause relapse of coeliac disease in specific patients. We have also examined the cross-reactivity of different cereal proteins in the assay and found good correlation with their known toxicity to patients with coeliac disease. SN - 0022-1759 UR - https://www.unboundmedicine.com/medline/citation/3559211/Monoclonal_antibody_ELISA_to_quantitate_wheat_gliadin_contamination_of_gluten_free_foods_ L2 - https://linkinghub.elsevier.com/retrieve/pii/0022-1759(87)90445-5 DB - PRIME DP - Unbound Medicine ER -