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Underground railway particulate matter and susceptibility to pneumococcal infection.
EBioMedicine. 2022 Jun; 80:104063.E

Abstract

BACKGROUND

Concentrations of particulate matter less than 10 microns (PM10) on underground railways are higher than those near urban roads. Traffic-related PM10 increases pneumococcal infection via increasing the expression of platelet-activating factor receptor (PAFR), a receptor co-opted by pneumococci to adhere to cells. To date, it is unknown whether underground railway PM10 increases pneumococcal infection. This study sought to determine the effect of London Underground (LU) PM10 on; i) pneumococcal adhesion to airway cells, and ii) susceptibility to pneumococcal disease.

METHODS

A549 cells and human primary airway epithelial cells were cultured with 20 µg/mL PM10 from the Bakerloo (B-PM10) and Jubilee (J-PM10) line platforms of Baker Street station. PAFR expression was assessed by flow cytometry, and pneumococcal adhesion by colony forming unit (CFU) counts. Traffic-related PM10 was collected next to a main road near the station's entrance. The PAFR blocker CV3988 and the antioxidant N-acetyl cysteine were used to assess the role of PAFR-mediated pneumococcal adhesion and oxidative stress respectively. Pneumococcal infection of mice was done after exposure to 3×80 μg doses of intranasal LU-PM10.

FINDINGS

In A549 cells, human primary nasal cells, and human primary bronchial epithelial cells, B-PM10 and J-PM10 increased PAFR expression and pneumococcal adhesion. Stimulated adhesion was abrogated by CV3988 and N-acetyl cysteine. Traffic-related PM10 stimulated increased adhesion compared with B-PM10. B-PM10 and J-PM10 increased lung and blood CFU and mortality in mice. Treatment of B-PM10-exposed mice with CV3988 reduced blood CFU.

INTERPRETATION

LU-PM10 increases pneumococcal adhesion to airway cells and susceptibility to invasive disease in mice.

FUNDING

The Medical College of Saint Bartholomew's Hospital Trust, and the UK Medical Research Council Programme Grant (MR/P011284/1).

Links

PMC Free PDF

Authors+Show Affiliations

Miyashita L
Centre for Genomics and Child Health, Blizard Institute, Queen Mary University of London, 4 Newark Street, London E1 2AT, UK.
Shears R
The Department of Clinical Infection, Microbiology and Immunology, University of Liverpool, Liverpool, UK.
Foley G
Centre for Genomics and Child Health, Blizard Institute, Queen Mary University of London, 4 Newark Street, London E1 2AT, UK.
Semple S
Institute for Social Marketing and Health, University of Stirling, Scotland.
Kadioglu A
The Department of Clinical Infection, Microbiology and Immunology, University of Liverpool, Liverpool, UK.
Grigg J
Centre for Genomics and Child Health, Blizard Institute, Queen Mary University of London, 4 Newark Street, London E1 2AT, UK. Electronic address: j.grigg@qmul.ac.uk.

MeSH

AnimalsCell LineCysteineHumansLungMiceParticulate MatterPneumococcal InfectionsStreptococcus pneumoniae

Pub Type(s)

Journal Article

Language

eng

PubMed ID

35598440

Citation

Miyashita, Lisa, et al. "Underground Railway Particulate Matter and Susceptibility to Pneumococcal Infection." EBioMedicine, vol. 80, 2022, p. 104063.
Miyashita L, Shears R, Foley G, et al. Underground railway particulate matter and susceptibility to pneumococcal infection. EBioMedicine. 2022;80:104063.
Miyashita, L., Shears, R., Foley, G., Semple, S., Kadioglu, A., & Grigg, J. (2022). Underground railway particulate matter and susceptibility to pneumococcal infection. EBioMedicine, 80, 104063. https://doi.org/10.1016/j.ebiom.2022.104063
Miyashita L, et al. Underground Railway Particulate Matter and Susceptibility to Pneumococcal Infection. EBioMedicine. 2022;80:104063. PubMed PMID: 35598440.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Underground railway particulate matter and susceptibility to pneumococcal infection. AU - Miyashita,Lisa, AU - Shears,Rebecca, AU - Foley,Gary, AU - Semple,Sean, AU - Kadioglu,Aras, AU - Grigg,Jonathan, Y1 - 2022/05/19/ PY - 2022/01/26/received PY - 2022/04/26/revised PY - 2022/04/30/accepted PY - 2022/5/23/pubmed PY - 2022/6/15/medline PY - 2022/5/22/entrez KW - London underground KW - Particulate matter KW - Pneumococcal infection SP - 104063 EP - 104063 JF - EBioMedicine JO - EBioMedicine VL - 80 N2 - BACKGROUND: Concentrations of particulate matter less than 10 microns (PM10) on underground railways are higher than those near urban roads. Traffic-related PM10 increases pneumococcal infection via increasing the expression of platelet-activating factor receptor (PAFR), a receptor co-opted by pneumococci to adhere to cells. To date, it is unknown whether underground railway PM10 increases pneumococcal infection. This study sought to determine the effect of London Underground (LU) PM10 on; i) pneumococcal adhesion to airway cells, and ii) susceptibility to pneumococcal disease. METHODS: A549 cells and human primary airway epithelial cells were cultured with 20 µg/mL PM10 from the Bakerloo (B-PM10) and Jubilee (J-PM10) line platforms of Baker Street station. PAFR expression was assessed by flow cytometry, and pneumococcal adhesion by colony forming unit (CFU) counts. Traffic-related PM10 was collected next to a main road near the station's entrance. The PAFR blocker CV3988 and the antioxidant N-acetyl cysteine were used to assess the role of PAFR-mediated pneumococcal adhesion and oxidative stress respectively. Pneumococcal infection of mice was done after exposure to 3×80 μg doses of intranasal LU-PM10. FINDINGS: In A549 cells, human primary nasal cells, and human primary bronchial epithelial cells, B-PM10 and J-PM10 increased PAFR expression and pneumococcal adhesion. Stimulated adhesion was abrogated by CV3988 and N-acetyl cysteine. Traffic-related PM10 stimulated increased adhesion compared with B-PM10. B-PM10 and J-PM10 increased lung and blood CFU and mortality in mice. Treatment of B-PM10-exposed mice with CV3988 reduced blood CFU. INTERPRETATION: LU-PM10 increases pneumococcal adhesion to airway cells and susceptibility to invasive disease in mice. FUNDING: The Medical College of Saint Bartholomew's Hospital Trust, and the UK Medical Research Council Programme Grant (MR/P011284/1). SN - 2352-3964 UR - https://www.unboundmedicine.com/medline/citation/35598440/Underground_railway_particulate_matter_and_susceptibility_to_pneumococcal_infection_ DB - PRIME DP - Unbound Medicine ER -