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Exploring of probiotic potential vaginal lactobacillus isolates from healthy women against Gardnerella vaginalis and Caenorhabditis elegans model testing.
J Appl Microbiol. 2022 May 28 [Online ahead of print]JA

Abstract

AIM

Lactobacillus species are the dominant microorganisms in the vaginal microbiota of healthy women and play an important role in the defence against pathogens. This study aimed to evaluate probiotic potential of Lactiplantibacillus plantarum strain P1 isolated from healthy woman's vaginal discharge for its further utilization as a promising candidate strain in the treatment of bacterial vaginosis caused by Gardnerella vaginalis.

METHODS AND RESULTS

Ten lactobacilli strains from a woman's vaginal discharge were evaluated for their probiotic potential, including growth capacity at different pH levels (pH 3.5-4.5), acid production, hydrogen peroxide production capacity, antibacterial activity and susceptibility to antibiotics. Moreover, in vitro safety assay haemolytic activity and mutagenicity were investigated for safety assessment. In vivo Caenorhabditis elegans infection model was used to investigate the anti-infection effect of selected isolates. We found that lactobacilli strain P1 showed strong growth ability in low acid environment, produced acid, hydrogen peroxide, had the strongest antibacterial activity against G. vaginalis and was highly susceptible to the tested antibiotics. When assayed for the safety, strain P1 showed no haemolytic activity and had no effect of mutagenicity. Moreover, P1 significantly increased the lifespan of C. elegans against G. vaginalis infection. Combined with the results of 16S rRNA gene sequencing, morphological and physiological characteristic, the strain was identified as Lactiplantibacillus plantarum.

CONCLUSION

Lactiplantibacillus plantarum strain P1 proves to be a promising candidate strain in the treatment of bacterial vaginosis caused by G. vaginalis.

SIGNIFICANCE AND IMPACT OF THE STUDY

Conventional antibiotic therapy for bacterial vaginosis has led to the accelerated process of bacterial drug resistance. Probiotics are potentially an alternative method for bacterial vaginosis therapy. This finding provides bacterial resources for keeping pathogens away from the vagina. We believe L. plantarum P1 may be used as vaginal probiotics and be useful to prevent or treat bacterial vaginitis.

Authors+Show Affiliations

Department of Pharmaceutical Microbiology, School of Life Science and Technology, China Pharmaceutical University, Nanjing, China.Animal, Plant and Food Inspection Center of Nanjing Customs, Nanjing, China.Department of Pharmaceutical Microbiology, School of Life Science and Technology, China Pharmaceutical University, Nanjing, China.Department of Pharmaceutical Microbiology, School of Life Science and Technology, China Pharmaceutical University, Nanjing, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

35633296

Citation

Li, Yunxia, et al. "Exploring of Probiotic Potential Vaginal Lactobacillus Isolates From Healthy Women Against Gardnerella Vaginalis and Caenorhabditis Elegans Model Testing." Journal of Applied Microbiology, 2022.
Li Y, Jiang Y, Lessing DJ, et al. Exploring of probiotic potential vaginal lactobacillus isolates from healthy women against Gardnerella vaginalis and Caenorhabditis elegans model testing. J Appl Microbiol. 2022.
Li, Y., Jiang, Y., Lessing, D. J., & Chu, W. (2022). Exploring of probiotic potential vaginal lactobacillus isolates from healthy women against Gardnerella vaginalis and Caenorhabditis elegans model testing. Journal of Applied Microbiology. https://doi.org/10.1111/jam.15643
Li Y, et al. Exploring of Probiotic Potential Vaginal Lactobacillus Isolates From Healthy Women Against Gardnerella Vaginalis and Caenorhabditis Elegans Model Testing. J Appl Microbiol. 2022 May 28; PubMed PMID: 35633296.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Exploring of probiotic potential vaginal lactobacillus isolates from healthy women against Gardnerella vaginalis and Caenorhabditis elegans model testing. AU - Li,Yunxia, AU - Jiang,Yan, AU - Lessing,Duncan James, AU - Chu,Weihua, Y1 - 2022/05/28/ PY - 2022/05/19/revised PY - 2022/04/18/received PY - 2022/05/24/accepted PY - 2022/5/29/pubmed PY - 2022/5/29/medline PY - 2022/5/28/entrez KW - Caenorhabditis elegans KW - Lactiplantibacillus plantarum KW - anti-infection KW - antibacterial activity KW - vaginal lactobacilli JF - Journal of applied microbiology JO - J Appl Microbiol N2 - AIM: Lactobacillus species are the dominant microorganisms in the vaginal microbiota of healthy women and play an important role in the defence against pathogens. This study aimed to evaluate probiotic potential of Lactiplantibacillus plantarum strain P1 isolated from healthy woman's vaginal discharge for its further utilization as a promising candidate strain in the treatment of bacterial vaginosis caused by Gardnerella vaginalis. METHODS AND RESULTS: Ten lactobacilli strains from a woman's vaginal discharge were evaluated for their probiotic potential, including growth capacity at different pH levels (pH 3.5-4.5), acid production, hydrogen peroxide production capacity, antibacterial activity and susceptibility to antibiotics. Moreover, in vitro safety assay haemolytic activity and mutagenicity were investigated for safety assessment. In vivo Caenorhabditis elegans infection model was used to investigate the anti-infection effect of selected isolates. We found that lactobacilli strain P1 showed strong growth ability in low acid environment, produced acid, hydrogen peroxide, had the strongest antibacterial activity against G. vaginalis and was highly susceptible to the tested antibiotics. When assayed for the safety, strain P1 showed no haemolytic activity and had no effect of mutagenicity. Moreover, P1 significantly increased the lifespan of C. elegans against G. vaginalis infection. Combined with the results of 16S rRNA gene sequencing, morphological and physiological characteristic, the strain was identified as Lactiplantibacillus plantarum. CONCLUSION: Lactiplantibacillus plantarum strain P1 proves to be a promising candidate strain in the treatment of bacterial vaginosis caused by G. vaginalis. SIGNIFICANCE AND IMPACT OF THE STUDY: Conventional antibiotic therapy for bacterial vaginosis has led to the accelerated process of bacterial drug resistance. Probiotics are potentially an alternative method for bacterial vaginosis therapy. This finding provides bacterial resources for keeping pathogens away from the vagina. We believe L. plantarum P1 may be used as vaginal probiotics and be useful to prevent or treat bacterial vaginitis. SN - 1365-2672 UR - https://www.unboundmedicine.com/medline/citation/35633296/Exploring_of_probiotic_potential_vaginal_lactobacillus_isolates_from_healthy_women_against_Gardnerella_vaginalis_and_Caenorhabditis_elegans_model_testing. L2 - https://doi.org/10.1111/jam.15643 DB - PRIME DP - Unbound Medicine ER -
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