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Development of wheat-Dasypyrum villosum T6V#4S·6AL translocation lines with enhanced inheritance for powdery mildew resistance.
Theor Appl Genet. 2022 Jul; 135(7):2423-2435.TA

Abstract

KEY MESSAGE

New translocation lines with T6V#4S·6AL in the Ph1 and ph1b backgrounds were developed with improved inheritance of powdery mildew resistance. The wheat-Dasypyrum villosum T6V#4S·6DL translocation line Pm97033, which exhibits strong powdery mildew (PM) resistance, was developed many years ago, but has limited application in wheat breeding. One of the major reasons for this is that the translocation chromosome has low transmission rate, which makes it difficult to obtain ideal genotype through recombination with other elite agronomic traits in a limited segregating population. Further modifications are thus needed to make better use of this genetic resource. In this study, Pm97033 and the T6V#2S·6AL translocation line NY-W were hybridized with the CS ph1b mutant, and two F1 hybrids were hybridized with each other. Then, plants homozygous for the ph1b deletion carrying the alien chromosome arm(s) 6V#2S and 6V#4S were identified from the segregating populations using molecular markers. New T6V#4S·6AL and T6V#2-6V#4S·6AL translocations were identified by molecular markers and confirmed by genomic in situ hybridization (GISH). Individuals that were heterozygous or homozygous for the translocation chromosome in Ph1 and ph1b backgrounds were obtained. The ratio of PM resistance vs. susceptibility in the self-pollinated heterozygous plants was 3:1, and the phenotype was completely consistent with the KASP genotyping. Thus, the new translocation chromosomes had higher transmission rate than the original T6V#4S·6DL, and so can be effectively applied in breeding programs.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.

Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.

Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.

Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.

Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China.

Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China. yexingguo@caas.cn. Key Laboratory of Ministry of Agriculture and Rural Affairs of China for Biology and Genetic Breeding of Triticeae Crops, Beijing, 100081, China. yexingguo@caas.cn.

Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100081, China. linzhishan@caas.cn. National Key Facility of Crop Gene Resources and Genetic Improvement, Beijing, 100081, China. linzhishan@caas.cn.

MeSH

Chromosomes, PlantDisease ResistancePlant BreedingPlant DiseasesPoaceaeTranslocation, GeneticTriticum

Pub Type(s)

Journal Article

Language

eng

PubMed ID

35644815

Citation

Ma, Xiaolan, et al. "Development of wheat-Dasypyrum Villosum T6V#4S·6AL Translocation Lines With Enhanced Inheritance for Powdery Mildew Resistance." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 135, no. 7, 2022, pp. 2423-2435.

Ma X, Chang Y, Chen J, et al. Development of wheat-Dasypyrum villosum T6V#4S·6AL translocation lines with enhanced inheritance for powdery mildew resistance. Theor Appl Genet. 2022;135(7):2423-2435.

Ma, X., Chang, Y., Chen, J., Yu, M., Wang, B., Ye, X., & Lin, Z. (2022). Development of wheat-Dasypyrum villosum T6V#4S·6AL translocation lines with enhanced inheritance for powdery mildew resistance. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 135(7), 2423-2435. https://doi.org/10.1007/s00122-022-04124-w

Ma X, et al. Development of wheat-Dasypyrum Villosum T6V#4S·6AL Translocation Lines With Enhanced Inheritance for Powdery Mildew Resistance. Theor Appl Genet. 2022;135(7):2423-2435. PubMed PMID: 35644815.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Development of wheat-Dasypyrum villosum T6V#4S·6AL translocation lines with enhanced inheritance for powdery mildew resistance. AU - Ma,Xiaolan, AU - Chang,Yanan, AU - Chen,Jingnan, AU - Yu,Mei, AU - Wang,Baicui, AU - Ye,Xingguo, AU - Lin,Zhishan, Y1 - 2022/05/30/ PY - 2022/02/15/received PY - 2022/05/06/accepted PY - 2022/6/2/pubmed PY - 2022/7/14/medline PY - 2022/6/1/entrez SP - 2423 EP - 2435 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 135 IS - 7 N2 - KEY MESSAGE: New translocation lines with T6V#4S·6AL in the Ph1 and ph1b backgrounds were developed with improved inheritance of powdery mildew resistance. The wheat-Dasypyrum villosum T6V#4S·6DL translocation line Pm97033, which exhibits strong powdery mildew (PM) resistance, was developed many years ago, but has limited application in wheat breeding. One of the major reasons for this is that the translocation chromosome has low transmission rate, which makes it difficult to obtain ideal genotype through recombination with other elite agronomic traits in a limited segregating population. Further modifications are thus needed to make better use of this genetic resource. In this study, Pm97033 and the T6V#2S·6AL translocation line NY-W were hybridized with the CS ph1b mutant, and two F1 hybrids were hybridized with each other. Then, plants homozygous for the ph1b deletion carrying the alien chromosome arm(s) 6V#2S and 6V#4S were identified from the segregating populations using molecular markers. New T6V#4S·6AL and T6V#2-6V#4S·6AL translocations were identified by molecular markers and confirmed by genomic in situ hybridization (GISH). Individuals that were heterozygous or homozygous for the translocation chromosome in Ph1 and ph1b backgrounds were obtained. The ratio of PM resistance vs. susceptibility in the self-pollinated heterozygous plants was 3:1, and the phenotype was completely consistent with the KASP genotyping. Thus, the new translocation chromosomes had higher transmission rate than the original T6V#4S·6DL, and so can be effectively applied in breeding programs. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/35644815/Development_of_wheat_Dasypyrum_villosum_T6V DB - PRIME DP - Unbound Medicine ER -