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A disposable printed amperometric biosensor for clinical evaluation of creatinine in renal function detection.
Talanta. 2022 Oct 01; 248:123592.T

Abstract

In clinical practice, sera creatinine level is regarded as a crucial biomarker for the diagnosis, staging and monitoring of kidney disease. An amperometric biosensor is rapid, accurate, and cost-effective, with a portability and a simple operation. Herein, we report for the firsttime a disposable, printed amperometric biosensor for the clinical evaluation of creatinine in renal function detection. The sensor is constructed based on Prussian blue/carbon-graphite paste as the working electrode and the immobilization of creatinine amidohydrolase, creatine amidinohydrolase and sarcosine oxidase. The creatinine biosensor shows a linear detection range from 0.05 to 1.4 mM with a detection time of about 3 min. In addition, the sensor shows a high stability that can maintain above 86% of the initial activity after being stored for over 4 months. Moreover, the sensor shows almost the same results as those with the Jaffe method for measuring the real blood samples. We anticipate that the creatinine biosensor could be widely used in the medical and healthcare areas, especially for at-home testing and onsite medical examinations.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Dong Y
Department of Medicine, Renal Division, Hospital 1, Peking University, Beijing, 100034, PR China; School of Materials Science and Engineering, Peking University, Beijing, 100871, PR China.
Luo X
School of Materials Science and Engineering, Peking University, Beijing, 100871, PR China.
Liu Y
School of Materials Science and Engineering, Peking University, Beijing, 100871, PR China.
Yan C
Department of Clinical Laboratory, Hospital 1, Peking University, Beijing, 100034, PR China.
Li H
Department of Clinical Laboratory, Hospital 1, Peking University, Beijing, 100034, PR China.
Lv J
Department of Medicine, Renal Division, Hospital 1, Peking University, Beijing, 100034, PR China. Electronic address: jichenglv75@gmail.com.
Yang L
Department of Medicine, Renal Division, Hospital 1, Peking University, Beijing, 100034, PR China.
Cui Y
School of Materials Science and Engineering, Peking University, Beijing, 100871, PR China. Electronic address: ycui@pku.edu.cn.

MeSH

Biosensing TechniquesCreatinineElectrodesEnzymes, ImmobilizedKidneySarcosine Oxidase

Pub Type(s)

Journal Article

Language

eng

PubMed ID

35671549

Citation

Dong, Yaping, et al. "A Disposable Printed Amperometric Biosensor for Clinical Evaluation of Creatinine in Renal Function Detection." Talanta, vol. 248, 2022, p. 123592.
Dong Y, Luo X, Liu Y, et al. A disposable printed amperometric biosensor for clinical evaluation of creatinine in renal function detection. Talanta. 2022;248:123592.
Dong, Y., Luo, X., Liu, Y., Yan, C., Li, H., Lv, J., Yang, L., & Cui, Y. (2022). A disposable printed amperometric biosensor for clinical evaluation of creatinine in renal function detection. Talanta, 248, 123592. https://doi.org/10.1016/j.talanta.2022.123592
Dong Y, et al. A Disposable Printed Amperometric Biosensor for Clinical Evaluation of Creatinine in Renal Function Detection. Talanta. 2022 Oct 1;248:123592. PubMed PMID: 35671549.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A disposable printed amperometric biosensor for clinical evaluation of creatinine in renal function detection. AU - Dong,Yaping, AU - Luo,Xiaojin, AU - Liu,Yiqun, AU - Yan,Cunling, AU - Li,Haixia, AU - Lv,Jicheng, AU - Yang,Li, AU - Cui,Yue, Y1 - 2022/05/27/ PY - 2021/09/23/received PY - 2022/03/01/revised PY - 2022/05/25/accepted PY - 2022/6/8/pubmed PY - 2022/6/22/medline PY - 2022/6/7/entrez KW - Amperometric KW - Biosensor KW - Creatinine KW - Enzyme KW - Kidney SP - 123592 EP - 123592 JF - Talanta JO - Talanta VL - 248 N2 - In clinical practice, sera creatinine level is regarded as a crucial biomarker for the diagnosis, staging and monitoring of kidney disease. An amperometric biosensor is rapid, accurate, and cost-effective, with a portability and a simple operation. Herein, we report for the firsttime a disposable, printed amperometric biosensor for the clinical evaluation of creatinine in renal function detection. The sensor is constructed based on Prussian blue/carbon-graphite paste as the working electrode and the immobilization of creatinine amidohydrolase, creatine amidinohydrolase and sarcosine oxidase. The creatinine biosensor shows a linear detection range from 0.05 to 1.4 mM with a detection time of about 3 min. In addition, the sensor shows a high stability that can maintain above 86% of the initial activity after being stored for over 4 months. Moreover, the sensor shows almost the same results as those with the Jaffe method for measuring the real blood samples. We anticipate that the creatinine biosensor could be widely used in the medical and healthcare areas, especially for at-home testing and onsite medical examinations. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/35671549/A_disposable_printed_amperometric_biosensor_for_clinical_evaluation_of_creatinine_in_renal_function_detection_ DB - PRIME DP - Unbound Medicine ER -