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Effect of bisbenzylisoquinoline (biscoclaurine) alkaloids on multidrug resistance in KB human cancer cells.
Cancer Res. 1987 May 01; 47(9):2413-6.CR

Abstract

Cepharanthine, a bisbenzylisoquinoline (biscoclaurine) alkaloid, completely overcomes resistance of a multidrug-resistant subline, ChR-24, derived from human KB carcinoma cells, to vincristine, actinomycin D, and daunomycin, and partially overcomes resistance to Adriamycin. Another biscoclaurine alkaloid, berbamine, partially overcomes resistance to these anticancer agents. Accumulation of [3H]daunomycin in ChR-24 cells is about 10% of that in both the parental KB and revertant cell line (Rev-2) which is derived from ChR-24. Cepharanthine prominently increases the accumulation of daunomycin in resistant ChR-24 cells, but not in parental KB and Rev-2 cells. Enhanced efflux of daunomycin from the resistant cells is completely inhibited by cepharanthine. Cellular uptake of [3H]daunomycin is not significantly affected in the resistant cells by cepharanthine. Accumulation of [3H]cepharanthine is observed at similar levels in both KB and ChR-24. Phosphatidylserine specifically inhibited the accumulation of [3H]cepharanthine in KB and ChR-24 cells when tested by adding various phospholipids such as phosphatidylserine, phosphatidylcholine, phosphatidylethanolamine, and sphingomyelin to culture medium. The enhanced accumulation of [3H]daunomycin in cepharanthine-treated ChR-24 cells is inhibited in the presence of 20 micrograms/ml phosphatidylserine. Cepharanthine may overcome multidrug resistance by binding to phosphatidylserine in the plasma membrane and perturbing membrane function.

Authors

Shiraishi N
No affiliation info available
Akiyama S
No affiliation info available
Nakagawa M
No affiliation info available
Kobayashi M
No affiliation info available
Kuwano M
No affiliation info available

MeSH

AlkaloidsBenzylisoquinolinesCarcinoma, Squamous CellDactinomycinDaunorubicinDoxorubicinDrug ResistanceHumansKB CellsPhospholipidsVincristine

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

3567927

Citation

Shiraishi, N, et al. "Effect of Bisbenzylisoquinoline (biscoclaurine) Alkaloids On Multidrug Resistance in KB Human Cancer Cells." Cancer Research, vol. 47, no. 9, 1987, pp. 2413-6.
Shiraishi N, Akiyama S, Nakagawa M, et al. Effect of bisbenzylisoquinoline (biscoclaurine) alkaloids on multidrug resistance in KB human cancer cells. Cancer Res. 1987;47(9):2413-6.
Shiraishi, N., Akiyama, S., Nakagawa, M., Kobayashi, M., & Kuwano, M. (1987). Effect of bisbenzylisoquinoline (biscoclaurine) alkaloids on multidrug resistance in KB human cancer cells. Cancer Research, 47(9), 2413-6.
Shiraishi N, et al. Effect of Bisbenzylisoquinoline (biscoclaurine) Alkaloids On Multidrug Resistance in KB Human Cancer Cells. Cancer Res. 1987 May 1;47(9):2413-6. PubMed PMID: 3567927.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of bisbenzylisoquinoline (biscoclaurine) alkaloids on multidrug resistance in KB human cancer cells. AU - Shiraishi,N, AU - Akiyama,S, AU - Nakagawa,M, AU - Kobayashi,M, AU - Kuwano,M, PY - 1987/5/1/pubmed PY - 1987/5/1/medline PY - 1987/5/1/entrez SP - 2413 EP - 6 JF - Cancer research JO - Cancer Res VL - 47 IS - 9 N2 - Cepharanthine, a bisbenzylisoquinoline (biscoclaurine) alkaloid, completely overcomes resistance of a multidrug-resistant subline, ChR-24, derived from human KB carcinoma cells, to vincristine, actinomycin D, and daunomycin, and partially overcomes resistance to Adriamycin. Another biscoclaurine alkaloid, berbamine, partially overcomes resistance to these anticancer agents. Accumulation of [3H]daunomycin in ChR-24 cells is about 10% of that in both the parental KB and revertant cell line (Rev-2) which is derived from ChR-24. Cepharanthine prominently increases the accumulation of daunomycin in resistant ChR-24 cells, but not in parental KB and Rev-2 cells. Enhanced efflux of daunomycin from the resistant cells is completely inhibited by cepharanthine. Cellular uptake of [3H]daunomycin is not significantly affected in the resistant cells by cepharanthine. Accumulation of [3H]cepharanthine is observed at similar levels in both KB and ChR-24. Phosphatidylserine specifically inhibited the accumulation of [3H]cepharanthine in KB and ChR-24 cells when tested by adding various phospholipids such as phosphatidylserine, phosphatidylcholine, phosphatidylethanolamine, and sphingomyelin to culture medium. The enhanced accumulation of [3H]daunomycin in cepharanthine-treated ChR-24 cells is inhibited in the presence of 20 micrograms/ml phosphatidylserine. Cepharanthine may overcome multidrug resistance by binding to phosphatidylserine in the plasma membrane and perturbing membrane function. SN - 0008-5472 UR - https://www.unboundmedicine.com/medline/citation/3567927/Effect_of_bisbenzylisoquinoline__biscoclaurine__alkaloids_on_multidrug_resistance_in_KB_human_cancer_cells_ DB - PRIME DP - Unbound Medicine ER -