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Cytogenetic Characterization and Molecular Marker Development for a Wheat-T. boeoticum 4Ab (4B) Disomic Substitution Line with Stripe Rust Resistance.
Plant Dis. 2023 Jan; 107(1):125-130.PD

Abstract

Triticum boeoticum (2n = 2x = 14, AbAb) is an important relative of wheat. This species tolerates many different types of environmental stresses, including drought, salt, and pathogenic infection, and is lower in dietary fiber and higher in antioxidants, protein (15 to 18%), lipids, and trace elements than common wheat. However, the gene transfer rate from this species to common wheat is low, and few species-specific molecular markers are available. In this study, the wheat-T. boeoticum substitution line Z1889, derived from a cross between the common wheat cultivar Crocus and T. boeoticum line G52, was identified using multicolor fluorescence in situ hybridization, multicolor genomic in situ hybridization, and a 55K single-nucleotide polymorphism array. Z1889 was revealed to be a 4Ab (4B) substitution line with a high degree of resistance to stripe rust pathogen strains prevalent in China. In addition, 22 4Ab chromosome-specific molecular markers and 11 T. boeoticum genome-specific molecular markers were developed from 1,145 4Ab chromosome-specific fragments by comparing the sequences generated by specific-length amplified fragment sequencing, with an efficiency of up to 55.0%. Furthermore, the specificity of these markers was verified in four species containing the Ab genome. These markers not only can be used for the detection of the 4Ab chromosome but also provide a basis for molecular marker-assisted, selection-based breeding in wheat.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, China.

State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, China. Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, China.

Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, China.

Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, China.

Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, China.

Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, China.

Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, China.

Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, China.

Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, China.

Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, China.

State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, China. Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, China.

Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, China.

Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, China.

Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, China.

Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, China.

State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, China. Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, China.

State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, China. Triticeae Research Institute, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, China.

MeSH

TriticumIn Situ Hybridization, FluorescenceDisease ResistancePlant BreedingBasidiomycotaGenetic Markers

Pub Type(s)

Journal Article

Language

eng

PubMed ID

35698253

Citation

Liu, Xin, et al. "Cytogenetic Characterization and Molecular Marker Development for a Wheat-T. Boeoticum 4Ab (4B) Disomic Substitution Line With Stripe Rust Resistance." Plant Disease, vol. 107, no. 1, 2023, pp. 125-130.

Liu X, Chen L, Zhang M, et al. Cytogenetic Characterization and Molecular Marker Development for a Wheat-T. boeoticum 4Ab (4B) Disomic Substitution Line with Stripe Rust Resistance. Plant Dis. 2023;107(1):125-130.

Liu, X., Chen, L., Zhang, M., Li, H., Jiang, X., Zhang, J., Jia, Z., Ma, P., Hao, M., Jiang, B., Huang, L., Ning, S., Yuan, Z., Chen, X., Chen, X., Liu, D., & Zhang, L. (2023). Cytogenetic Characterization and Molecular Marker Development for a Wheat-T. boeoticum 4Ab (4B) Disomic Substitution Line with Stripe Rust Resistance. Plant Disease, 107(1), 125-130. https://doi.org/10.1094/PDIS-04-22-0865-RE

Liu X, et al. Cytogenetic Characterization and Molecular Marker Development for a Wheat-T. Boeoticum 4Ab (4B) Disomic Substitution Line With Stripe Rust Resistance. Plant Dis. 2023;107(1):125-130. PubMed PMID: 35698253.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Cytogenetic Characterization and Molecular Marker Development for a Wheat-T. boeoticum 4Ab (4B) Disomic Substitution Line with Stripe Rust Resistance. AU - Liu,Xin, AU - Chen,Longyu, AU - Zhang,Minghu, AU - Li,Hui, AU - Jiang,Xiaomei, AU - Zhang,Junqing, AU - Jia,Zhenjiao, AU - Ma,Pan, AU - Hao,Ming, AU - Jiang,Bo, AU - Huang,Lin, AU - Ning,Shunzong, AU - Yuan,Zhongwei, AU - Chen,Xuejiao, AU - Chen,Xue, AU - Liu,Dengcai, AU - Zhang,Lianquan, Y1 - 2023/01/19/ PY - 2022/6/14/pubmed PY - 2022/6/14/medline PY - 2022/6/13/entrez KW - 55K SNP KW - FISH KW - GISH KW - SLAF-seq KW - Triticum boeoticum KW - stripe rust KW - substitution line SP - 125 EP - 130 JF - Plant disease JO - Plant Dis VL - 107 IS - 1 N2 - Triticum boeoticum (2n = 2x = 14, AbAb) is an important relative of wheat. This species tolerates many different types of environmental stresses, including drought, salt, and pathogenic infection, and is lower in dietary fiber and higher in antioxidants, protein (15 to 18%), lipids, and trace elements than common wheat. However, the gene transfer rate from this species to common wheat is low, and few species-specific molecular markers are available. In this study, the wheat-T. boeoticum substitution line Z1889, derived from a cross between the common wheat cultivar Crocus and T. boeoticum line G52, was identified using multicolor fluorescence in situ hybridization, multicolor genomic in situ hybridization, and a 55K single-nucleotide polymorphism array. Z1889 was revealed to be a 4Ab (4B) substitution line with a high degree of resistance to stripe rust pathogen strains prevalent in China. In addition, 22 4Ab chromosome-specific molecular markers and 11 T. boeoticum genome-specific molecular markers were developed from 1,145 4Ab chromosome-specific fragments by comparing the sequences generated by specific-length amplified fragment sequencing, with an efficiency of up to 55.0%. Furthermore, the specificity of these markers was verified in four species containing the Ab genome. These markers not only can be used for the detection of the 4Ab chromosome but also provide a basis for molecular marker-assisted, selection-based breeding in wheat. SN - 0191-2917 UR - https://www.unboundmedicine.com/medline/citation/35698253/Cytogenetic_Characterization_and_Molecular_Marker_Development_for_a_Wheat_T__boeoticum_4Ab__4B__Disomic_Substitution_Line_with_Stripe_Rust_Resistance_ DB - PRIME DP - Unbound Medicine ER -