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Subacute sclerosing panencephalitis: detection of measles antibody in serum and cerebrospinal fluid by enzyme-linked immunosorbent assay, complement fixation and hemagglutination inhibition.
Microbiologica. 1987 Jan; 10(1):111-8.M

Abstract

Subacute sclerosing panencephalitis (SSPE), a slowly progressive central nervous system disease, is related to the measles virus. Because laboratory immunoassays are important in the diagnosis of the disease, we compared enzyme-linked immunosorbent assay (ELISA), complement fixation (CF), and hemagglutination inhibition (HI) for the detection of measles antibody in the serum and cerebrospinal fluid of patients with SSPE and in controls. Differences in the sensitivity of the three methods were not observed in the serum and cerebrospinal fluid from patients because all specimens were positive, but ELISA and CF were more sensitive that HI for both serum and cerebrospinal fluid from controls. ELISA also distinguished between subacute sclerosing panencephalitis cases and controls with high measles antibody levels (acute measles). Thus, it appears that ELISA for measles antibody is superior to CF and HI in the laboratory diagnosis of SSPE and is the preferred method.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

3574144

Citation

Ferrante, P, et al. "Subacute Sclerosing Panencephalitis: Detection of Measles Antibody in Serum and Cerebrospinal Fluid By Enzyme-linked Immunosorbent Assay, Complement Fixation and Hemagglutination Inhibition." Microbiologica, vol. 10, no. 1, 1987, pp. 111-8.
Ferrante P, Achilli G, Gerna G, et al. Subacute sclerosing panencephalitis: detection of measles antibody in serum and cerebrospinal fluid by enzyme-linked immunosorbent assay, complement fixation and hemagglutination inhibition. Microbiologica. 1987;10(1):111-8.
Ferrante, P., Achilli, G., Gerna, G., & Bergamini, F. (1987). Subacute sclerosing panencephalitis: detection of measles antibody in serum and cerebrospinal fluid by enzyme-linked immunosorbent assay, complement fixation and hemagglutination inhibition. Microbiologica, 10(1), 111-8.
Ferrante P, et al. Subacute Sclerosing Panencephalitis: Detection of Measles Antibody in Serum and Cerebrospinal Fluid By Enzyme-linked Immunosorbent Assay, Complement Fixation and Hemagglutination Inhibition. Microbiologica. 1987;10(1):111-8. PubMed PMID: 3574144.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Subacute sclerosing panencephalitis: detection of measles antibody in serum and cerebrospinal fluid by enzyme-linked immunosorbent assay, complement fixation and hemagglutination inhibition. AU - Ferrante,P, AU - Achilli,G, AU - Gerna,G, AU - Bergamini,F, PY - 1987/1/1/pubmed PY - 1987/1/1/medline PY - 1987/1/1/entrez SP - 111 EP - 8 JF - Microbiologica JO - Microbiologica VL - 10 IS - 1 N2 - Subacute sclerosing panencephalitis (SSPE), a slowly progressive central nervous system disease, is related to the measles virus. Because laboratory immunoassays are important in the diagnosis of the disease, we compared enzyme-linked immunosorbent assay (ELISA), complement fixation (CF), and hemagglutination inhibition (HI) for the detection of measles antibody in the serum and cerebrospinal fluid of patients with SSPE and in controls. Differences in the sensitivity of the three methods were not observed in the serum and cerebrospinal fluid from patients because all specimens were positive, but ELISA and CF were more sensitive that HI for both serum and cerebrospinal fluid from controls. ELISA also distinguished between subacute sclerosing panencephalitis cases and controls with high measles antibody levels (acute measles). Thus, it appears that ELISA for measles antibody is superior to CF and HI in the laboratory diagnosis of SSPE and is the preferred method. SN - 0391-5352 UR - https://www.unboundmedicine.com/medline/citation/3574144/Subacute_sclerosing_panencephalitis:_detection_of_measles_antibody_in_serum_and_cerebrospinal_fluid_by_enzyme_linked_immunosorbent_assay_complement_fixation_and_hemagglutination_inhibition_ DB - PRIME DP - Unbound Medicine ER -