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A Pilot Study on Donor Human Milk Microbiota: A Comparison with Preterm Human Milk Microbiota and the Effect of Pasteurization.
Nutrients. 2022 Jun 15; 14(12)N

Abstract

Human milk (HM) is the best feeding option for preterm infants; however, when mother's own milk (MOM) is not available, pasteurized donor human milk (DHM) is the best alternative. In this study, we profiled DHM microbiota (19 samples) using 16S rRNA amplicon sequencing and compared its compositional features with the MOM microbiota (14 samples) from mothers who delivered prematurely (PT-MOM). As a secondary study aim, we assessed the specific effect of pasteurization on the characteristics of the DHM microbiota. DHM showed significantly higher alpha diversity and significant segregation from PT-MOM. Compositionally, the PT-MOM microbiota had a significantly higher proportion of Staphylococcus than DHM, with Streptococcus tending to be and Pseudomonas being significantly overrepresented in DHM compared with the PT-MOM samples. Furthermore, pasteurization affected the HM microbiota structure, with a trend towards greater biodiversity and some compositional differences following pasteurization. This pilot study provided further evidence on the HM microbial ecosystem, demonstrating that the DHM microbiota differs from the PT-MOM microbiota, possibly due to inherent differences between HM donors and mothers delivering prematurely, and that pasteurization per se impacts the HM microbiota. Knowledge about HM microbiota needs to be acquired by investigating the effect of DHM processing to develop strategies aimed at improving DHM quality while guaranteeing its microbiological safety.

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Authors+Show Affiliations

Beghetti I
Department of Medical and Surgical Sciences, University of Bologna, 40138 Bologna, Italy. Neonatal Intensive Care Unit, IRCCS Azienda Ospedaliero Universitaria di Bologna, 40138 Bologna, Italy.
Barone M
Department of Medical and Surgical Sciences, University of Bologna, 40138 Bologna, Italy.
De Fazio L
Department of Medical and Surgical Sciences, University of Bologna, 40138 Bologna, Italy. Neonatal Intensive Care Unit, IRCCS Azienda Ospedaliero Universitaria di Bologna, 40138 Bologna, Italy.
Laderchi E
Department of Medical and Surgical Sciences, University of Bologna, 40138 Bologna, Italy. Neonatal Intensive Care Unit, IRCCS Azienda Ospedaliero Universitaria di Bologna, 40138 Bologna, Italy.
Biagi E
Unit of Microbiome Science and Biotechnology, Department of Pharmacy and Biotechnology, University of Bologna, 40126 Bologna, Italy.
Turroni S
Unit of Microbiome Science and Biotechnology, Department of Pharmacy and Biotechnology, University of Bologna, 40126 Bologna, Italy.
Brigidi P
Department of Medical and Surgical Sciences, University of Bologna, 40138 Bologna, Italy.
Pession A
Department of Medical and Surgical Sciences, University of Bologna, 40138 Bologna, Italy. Paediatric Unit, IRCCS Azienda Ospedaliero Universitaria di Bologna, Via Massarenti 11, 40138 Bologna, Italy.
Corvaglia L
Department of Medical and Surgical Sciences, University of Bologna, 40138 Bologna, Italy. Neonatal Intensive Care Unit, IRCCS Azienda Ospedaliero Universitaria di Bologna, 40138 Bologna, Italy.
Aceti A
Department of Medical and Surgical Sciences, University of Bologna, 40138 Bologna, Italy. Neonatal Intensive Care Unit, IRCCS Azienda Ospedaliero Universitaria di Bologna, 40138 Bologna, Italy.

MeSH

HumansInfantInfant, NewbornInfant, PrematureMicrobiotaMilk BanksMilk, HumanPasteurizationPilot ProjectsRNA, Ribosomal, 16S

Pub Type(s)

Journal Article

Language

eng

PubMed ID

35745213

Citation

Beghetti, Isadora, et al. "A Pilot Study On Donor Human Milk Microbiota: a Comparison With Preterm Human Milk Microbiota and the Effect of Pasteurization." Nutrients, vol. 14, no. 12, 2022.
Beghetti I, Barone M, De Fazio L, et al. A Pilot Study on Donor Human Milk Microbiota: A Comparison with Preterm Human Milk Microbiota and the Effect of Pasteurization. Nutrients. 2022;14(12).
Beghetti, I., Barone, M., De Fazio, L., Laderchi, E., Biagi, E., Turroni, S., Brigidi, P., Pession, A., Corvaglia, L., & Aceti, A. (2022). A Pilot Study on Donor Human Milk Microbiota: A Comparison with Preterm Human Milk Microbiota and the Effect of Pasteurization. Nutrients, 14(12). https://doi.org/10.3390/nu14122483
Beghetti I, et al. A Pilot Study On Donor Human Milk Microbiota: a Comparison With Preterm Human Milk Microbiota and the Effect of Pasteurization. Nutrients. 2022 Jun 15;14(12) PubMed PMID: 35745213.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A Pilot Study on Donor Human Milk Microbiota: A Comparison with Preterm Human Milk Microbiota and the Effect of Pasteurization. AU - Beghetti,Isadora, AU - Barone,Monica, AU - De Fazio,Luigia, AU - Laderchi,Eleonora, AU - Biagi,Elena, AU - Turroni,Silvia, AU - Brigidi,Patrizia, AU - Pession,Andrea, AU - Corvaglia,Luigi, AU - Aceti,Arianna, Y1 - 2022/06/15/ PY - 2022/05/11/received PY - 2022/06/10/revised PY - 2022/06/13/accepted PY - 2022/6/24/entrez PY - 2022/6/25/pubmed PY - 2022/6/28/medline KW - donor human milk KW - human milk microbiota KW - pasteurization KW - very low birthweight preterm infants JF - Nutrients JO - Nutrients VL - 14 IS - 12 N2 - Human milk (HM) is the best feeding option for preterm infants; however, when mother's own milk (MOM) is not available, pasteurized donor human milk (DHM) is the best alternative. In this study, we profiled DHM microbiota (19 samples) using 16S rRNA amplicon sequencing and compared its compositional features with the MOM microbiota (14 samples) from mothers who delivered prematurely (PT-MOM). As a secondary study aim, we assessed the specific effect of pasteurization on the characteristics of the DHM microbiota. DHM showed significantly higher alpha diversity and significant segregation from PT-MOM. Compositionally, the PT-MOM microbiota had a significantly higher proportion of Staphylococcus than DHM, with Streptococcus tending to be and Pseudomonas being significantly overrepresented in DHM compared with the PT-MOM samples. Furthermore, pasteurization affected the HM microbiota structure, with a trend towards greater biodiversity and some compositional differences following pasteurization. This pilot study provided further evidence on the HM microbial ecosystem, demonstrating that the DHM microbiota differs from the PT-MOM microbiota, possibly due to inherent differences between HM donors and mothers delivering prematurely, and that pasteurization per se impacts the HM microbiota. Knowledge about HM microbiota needs to be acquired by investigating the effect of DHM processing to develop strategies aimed at improving DHM quality while guaranteeing its microbiological safety. SN - 2072-6643 UR - https://www.unboundmedicine.com/medline/citation/35745213/A_Pilot_Study_on_Donor_Human_Milk_Microbiota:_A_Comparison_with_Preterm_Human_Milk_Microbiota_and_the_Effect_of_Pasteurization_ DB - PRIME DP - Unbound Medicine ER -