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Toxicometabolomic profiling of resistant and susceptible western corn rootworm larvae feeding on Bt maize seedlings.
Sci Rep. 2022 07 08; 12(1):11639.SR

Abstract

The western corn rootworm (WCR), Diabrotica virgifera virgifera LeConte, is the most serious pest of maize (Zea mays L.) in the U.S. Corn Belt and parts of Europe. Transgenic maize hybrids expressing at least one of the four currently available insecticidal toxins from Bacillus thuringiensis (Bt) Berliner, currently the most widely adopted control method in continuous maize, have faltered due to the emergence of resistance. The resistance mechanisms of WCR to Bt toxins are not fully understood. We identified metabolic profiles of susceptible and resistant WCR larvae fed on maize hybrids expressing each of three available Cry3 proteins (eCry3Ab1, mCry3A, and Cry3Bb1) targeting corn rootworms and a control non-Bt maize via an untargeted metabolomics approach. Over 580 unique metabolites found in WCR larvae were classified into different pathways (amino acids, carbohydrates, cofactors and vitamins, energy, lipid, nucleotide, peptide, and xenobiotics). By exploring shifts in WCR larval metabolome exclusively by Bt toxins, several candidate metabolites and metabolic pathways were identified in susceptible and resistant larvae that may be involved in defense against or recovery from Bt ingestion by these larvae. These findings would provide mechanistic insights into altered metabolic pathways associated with the resistance mechanisms of WCR to Bt toxins.

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Authors+Show Affiliations

Huynh MP
Division of Plant Science and Technology, University of Missouri, Columbia, MO, USA. manhuynh@missouri.edu. Department of Plant Protection, Can Tho University, Can Tho, Vietnam. manhuynh@missouri.edu.
Hibbard BE
Plant Genetics Research Unit, USDA-Agricultural Research Service, Columbia, MO, USA.
Ho KV
Department of Chemistry, University of Missouri, Columbia, MO, USA. Molecular Imaging and Theranostics Center, University of Missouri, Columbia, MO, USA. Department of Food Technology, Can Tho University, Can Tho, Vietnam.
Shelby KS
Biological Control of Insects Research Laboratory, USDA-Agricultural Research Service, Columbia, MO, USA. kent.shelby@usda.gov.

MeSH

AnimalsBacillus thuringiensisBacillus thuringiensis ToxinsColeopteraEndotoxinsInsecticide ResistanceLarvaPest Control, BiologicalPlants, Genetically ModifiedSeedlingsZea mays

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

35804088

Citation

Huynh, Man P., et al. "Toxicometabolomic Profiling of Resistant and Susceptible Western Corn Rootworm Larvae Feeding On Bt Maize Seedlings." Scientific Reports, vol. 12, no. 1, 2022, p. 11639.
Huynh MP, Hibbard BE, Ho KV, et al. Toxicometabolomic profiling of resistant and susceptible western corn rootworm larvae feeding on Bt maize seedlings. Sci Rep. 2022;12(1):11639.
Huynh, M. P., Hibbard, B. E., Ho, K. V., & Shelby, K. S. (2022). Toxicometabolomic profiling of resistant and susceptible western corn rootworm larvae feeding on Bt maize seedlings. Scientific Reports, 12(1), 11639. https://doi.org/10.1038/s41598-022-15895-z
Huynh MP, et al. Toxicometabolomic Profiling of Resistant and Susceptible Western Corn Rootworm Larvae Feeding On Bt Maize Seedlings. Sci Rep. 2022 07 8;12(1):11639. PubMed PMID: 35804088.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Toxicometabolomic profiling of resistant and susceptible western corn rootworm larvae feeding on Bt maize seedlings. AU - Huynh,Man P, AU - Hibbard,Bruce E, AU - Ho,Khanh-Van, AU - Shelby,Kent S, Y1 - 2022/07/08/ PY - 2022/02/24/received PY - 2022/06/30/accepted PY - 2022/7/8/entrez PY - 2022/7/9/pubmed PY - 2022/7/14/medline SP - 11639 EP - 11639 JF - Scientific reports JO - Sci Rep VL - 12 IS - 1 N2 - The western corn rootworm (WCR), Diabrotica virgifera virgifera LeConte, is the most serious pest of maize (Zea mays L.) in the U.S. Corn Belt and parts of Europe. Transgenic maize hybrids expressing at least one of the four currently available insecticidal toxins from Bacillus thuringiensis (Bt) Berliner, currently the most widely adopted control method in continuous maize, have faltered due to the emergence of resistance. The resistance mechanisms of WCR to Bt toxins are not fully understood. We identified metabolic profiles of susceptible and resistant WCR larvae fed on maize hybrids expressing each of three available Cry3 proteins (eCry3Ab1, mCry3A, and Cry3Bb1) targeting corn rootworms and a control non-Bt maize via an untargeted metabolomics approach. Over 580 unique metabolites found in WCR larvae were classified into different pathways (amino acids, carbohydrates, cofactors and vitamins, energy, lipid, nucleotide, peptide, and xenobiotics). By exploring shifts in WCR larval metabolome exclusively by Bt toxins, several candidate metabolites and metabolic pathways were identified in susceptible and resistant larvae that may be involved in defense against or recovery from Bt ingestion by these larvae. These findings would provide mechanistic insights into altered metabolic pathways associated with the resistance mechanisms of WCR to Bt toxins. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/35804088/Toxicometabolomic_profiling_of_resistant_and_susceptible_western_corn_rootworm_larvae_feeding_on_Bt_maize_seedlings_ DB - PRIME DP - Unbound Medicine ER -