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Development and Molecular Cytogenetic Identification of Two Wheat-Aegilops geniculata Roth 7Mg Chromosome Substitution Lines with Resistance to Fusarium Head Blight, Powdery Mildew and Stripe Rust.
Int J Mol Sci. 2022 Jun 24; 23(13)IJ

Abstract

Fusarium head blight (Fhb), powdery mildew, and stripe rust are major wheat diseases globally. Aegilops geniculata Roth (UgUgMgMg, 2n = 4x = 28), a wild relative of common wheat, is valuable germplasm of disease resistance for wheat improvement and breeding. Here, we report the development and characterization of two substitution accessions with high resistance to powdery mildew, stripe rust and Fhb (W623 and W637) derived from hybrid progenies between Ae. geniculata and hexaploid wheat Chinese Spring (CS). Fluorescence in situ hybridization (FISH), Genomic in situ hybridizations (GISH), and sequential FISH-GISH studies indicated that the two substitution lines possess 40 wheat chromosomes and 2 Ae. geniculata chromosomes. Furthermore, compared that the wheat addition line parent W166, the 2 alien chromosomes from W623 and W637 belong to the 7Mg chromosomes of Ae. geniculata via sequential FISH-GISH and molecular marker analysis. Nullisomic-tetrasomic analysis for homoeologous group-7 of wheat and FISH revealed that the common wheat chromosomes 7A and 7B were replaced in W623 and W637, respectively. Consequently, lines W623, in which wheat chromosomes 7A were replaced by a pair of Ae. geniculata 7Mg chromosomes, and W637, which chromosomes 7B were substituted by chromosomes 7Mg, with resistance to Fhb, powdery mildew, and stripe rust. This study has determined that the chromosome 7Mg from Ae. geniculata exists genes resistant to Fhb and powdery mildew.

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Authors+Show Affiliations

College of Agronomy, Northwest A&F University, Yangling 712100, China.

College of Agronomy, Northwest A&F University, Yangling 712100, China.

College of Agronomy, Northwest A&F University, Yangling 712100, China.

College of Agronomy, Northwest A&F University, Yangling 712100, China.

College of Agronomy, Northwest A&F University, Yangling 712100, China.

College of Agronomy, Northwest A&F University, Yangling 712100, China. State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling 712100, China. Shaanxi Research Station of Crop Gene Resources and Germplasm Enhancement, Ministry of Agriculture, Yangling 712100, China.

College of Agronomy, Northwest A&F University, Yangling 712100, China. State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling 712100, China. Shaanxi Research Station of Crop Gene Resources and Germplasm Enhancement, Ministry of Agriculture, Yangling 712100, China.

College of Agronomy, Northwest A&F University, Yangling 712100, China. State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling 712100, China. Shaanxi Research Station of Crop Gene Resources and Germplasm Enhancement, Ministry of Agriculture, Yangling 712100, China.

College of Agronomy, Northwest A&F University, Yangling 712100, China. State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling 712100, China. Shaanxi Research Station of Crop Gene Resources and Germplasm Enhancement, Ministry of Agriculture, Yangling 712100, China.

College of Agronomy, Northwest A&F University, Yangling 712100, China. State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling 712100, China. Shaanxi Research Station of Crop Gene Resources and Germplasm Enhancement, Ministry of Agriculture, Yangling 712100, China.

College of Agronomy, Northwest A&F University, Yangling 712100, China. State Key Laboratory of Crop Stress Biology for Arid Areas, Yangling 712100, China. Shaanxi Research Station of Crop Gene Resources and Germplasm Enhancement, Ministry of Agriculture, Yangling 712100, China.

MeSH

AegilopsBasidiomycotaChromosomes, PlantDisease ResistanceErysipheFusariumIn Situ Hybridization, FluorescencePlant BreedingPlant DiseasesTriticum

Pub Type(s)

Journal Article

Language

eng

PubMed ID

35806057

Citation

Yang, Xiaoying, et al. "Development and Molecular Cytogenetic Identification of Two Wheat-Aegilops Geniculata Roth 7Mg Chromosome Substitution Lines With Resistance to Fusarium Head Blight, Powdery Mildew and Stripe Rust." International Journal of Molecular Sciences, vol. 23, no. 13, 2022.

Yang X, Xu M, Wang Y, et al. Development and Molecular Cytogenetic Identification of Two Wheat-Aegilops geniculata Roth 7Mg Chromosome Substitution Lines with Resistance to Fusarium Head Blight, Powdery Mildew and Stripe Rust. Int J Mol Sci. 2022;23(13).

Yang, X., Xu, M., Wang, Y., Cheng, X., Huang, C., Zhang, H., Li, T., Wang, C., Chen, C., Wang, Y., & Ji, W. (2022). Development and Molecular Cytogenetic Identification of Two Wheat-Aegilops geniculata Roth 7Mg Chromosome Substitution Lines with Resistance to Fusarium Head Blight, Powdery Mildew and Stripe Rust. International Journal of Molecular Sciences, 23(13). https://doi.org/10.3390/ijms23137056

Yang X, et al. Development and Molecular Cytogenetic Identification of Two Wheat-Aegilops Geniculata Roth 7Mg Chromosome Substitution Lines With Resistance to Fusarium Head Blight, Powdery Mildew and Stripe Rust. Int J Mol Sci. 2022 Jun 24;23(13) PubMed PMID: 35806057.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Development and Molecular Cytogenetic Identification of Two Wheat-Aegilops geniculata Roth 7Mg Chromosome Substitution Lines with Resistance to Fusarium Head Blight, Powdery Mildew and Stripe Rust. AU - Yang,Xiaoying, AU - Xu,Maoru, AU - Wang,Yongfu, AU - Cheng,Xiaofang, AU - Huang,Chenxi, AU - Zhang,Hong, AU - Li,Tingdong, AU - Wang,Changyou, AU - Chen,Chunhuan, AU - Wang,Yajuan, AU - Ji,Wanquan, Y1 - 2022/06/24/ PY - 2022/05/20/received PY - 2022/06/16/revised PY - 2022/06/20/accepted PY - 2022/7/9/entrez PY - 2022/7/10/pubmed PY - 2022/7/14/medline KW - Aegilops geniculata Roth KW - Fusarium head blight (Fhb) KW - powdery mildew KW - stripe rust KW - substitution lines JF - International journal of molecular sciences JO - Int J Mol Sci VL - 23 IS - 13 N2 - Fusarium head blight (Fhb), powdery mildew, and stripe rust are major wheat diseases globally. Aegilops geniculata Roth (UgUgMgMg, 2n = 4x = 28), a wild relative of common wheat, is valuable germplasm of disease resistance for wheat improvement and breeding. Here, we report the development and characterization of two substitution accessions with high resistance to powdery mildew, stripe rust and Fhb (W623 and W637) derived from hybrid progenies between Ae. geniculata and hexaploid wheat Chinese Spring (CS). Fluorescence in situ hybridization (FISH), Genomic in situ hybridizations (GISH), and sequential FISH-GISH studies indicated that the two substitution lines possess 40 wheat chromosomes and 2 Ae. geniculata chromosomes. Furthermore, compared that the wheat addition line parent W166, the 2 alien chromosomes from W623 and W637 belong to the 7Mg chromosomes of Ae. geniculata via sequential FISH-GISH and molecular marker analysis. Nullisomic-tetrasomic analysis for homoeologous group-7 of wheat and FISH revealed that the common wheat chromosomes 7A and 7B were replaced in W623 and W637, respectively. Consequently, lines W623, in which wheat chromosomes 7A were replaced by a pair of Ae. geniculata 7Mg chromosomes, and W637, which chromosomes 7B were substituted by chromosomes 7Mg, with resistance to Fhb, powdery mildew, and stripe rust. This study has determined that the chromosome 7Mg from Ae. geniculata exists genes resistant to Fhb and powdery mildew. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/35806057/Development_and_Molecular_Cytogenetic_Identification_of_Two_Wheat_Aegilops_geniculata_Roth_7Mg_Chromosome_Substitution_Lines_with_Resistance_to_Fusarium_Head_Blight_Powdery_Mildew_and_Stripe_Rust_ DB - PRIME DP - Unbound Medicine ER -