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Association of voltage-gated sodium channel mutations with field-evolved pyrethroid resistant phenotypes in soybean aphid and genetic markers for their detection.
Sci Rep. 2022 07 14; 12(1):12020.SR

Abstract

The frequent use of insecticides to manage soybean aphids, Aphis glycines (Hemiptera: Aphididae), in the United States has contributed to field-evolved resistance. Pyrethroid-resistant aphids have nonsynonymous mutations in the voltage-gated sodium channel (vgsc). We identified a leucine to phenylalanine mutation at position 1014 (L1014F) and a methionine to isoleucine mutation (M918I) of the A. glycines vgsc, both suspected of conferring knockdown resistance (kdr) to lambda-cyhalothrin. We developed molecular markers to identify these mutations in insecticide-resistant aphids. We determined that A. glycines which survived exposure to a diagnostic concentration of lambda-cyhalothrin and bifenthrin via glass-vial bioassays had these mutations, and showed significant changes in the resistance allele frequency between samples collected before and after field application of lambda-cyhalothrin. Thus, a strong association was revealed between aphids with L1014F and M918I vgsc mutations and survival following exposure to pyrethroids. Specifically, the highest survival was observed for aphids with the kdr (L1014F) and heterozygote super-kdr (L1014F + M918I) genotypes following laboratory bioassays and in-field application of lambda-cyhalothrin. These genetic markers could be used as a diagnostic tool for detecting insecticide-resistant A. glycines and monitoring the geographic distribution of pyrethroid resistance. We discuss how generating these types of data could improve our efforts to mitigate the effects of pyrethroid resistance on crop production.

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Authors+Show Affiliations

Valmorbida I
Department of Entomology, Iowa State University, Ames, IA, USA. ivairvalmorbida@gmail.com.
Hohenstein JD
Department of Entomology, Iowa State University, Ames, IA, USA.
Coates BS
United States Department of Agriculture, Agricultural Research Service, Corn Insects and Crop Genetics Research Unit, Ames, IA, USA.
Bevilaqua JG
Department of Crop Protection, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil.
Menger J
Department of Entomology, University of Minnesota, Saint Paul, MN, USA.
Hodgson EW
Department of Entomology, Iowa State University, Ames, IA, USA.
Koch RL
Department of Entomology, University of Minnesota, Saint Paul, MN, USA.
O'Neal ME
Department of Entomology, Iowa State University, Ames, IA, USA. oneal@iastate.edu.

MeSH

AnimalsAphidsGenetic MarkersInsecticide ResistanceInsecticidesMutationPhenotypePyrethrinsSoybeansVoltage-Gated Sodium Channels

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

35835854

Citation

Valmorbida, Ivair, et al. "Association of Voltage-gated Sodium Channel Mutations With Field-evolved Pyrethroid Resistant Phenotypes in Soybean Aphid and Genetic Markers for Their Detection." Scientific Reports, vol. 12, no. 1, 2022, p. 12020.
Valmorbida I, Hohenstein JD, Coates BS, et al. Association of voltage-gated sodium channel mutations with field-evolved pyrethroid resistant phenotypes in soybean aphid and genetic markers for their detection. Sci Rep. 2022;12(1):12020.
Valmorbida, I., Hohenstein, J. D., Coates, B. S., Bevilaqua, J. G., Menger, J., Hodgson, E. W., Koch, R. L., & O'Neal, M. E. (2022). Association of voltage-gated sodium channel mutations with field-evolved pyrethroid resistant phenotypes in soybean aphid and genetic markers for their detection. Scientific Reports, 12(1), 12020. https://doi.org/10.1038/s41598-022-16366-1
Valmorbida I, et al. Association of Voltage-gated Sodium Channel Mutations With Field-evolved Pyrethroid Resistant Phenotypes in Soybean Aphid and Genetic Markers for Their Detection. Sci Rep. 2022 07 14;12(1):12020. PubMed PMID: 35835854.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Association of voltage-gated sodium channel mutations with field-evolved pyrethroid resistant phenotypes in soybean aphid and genetic markers for their detection. AU - Valmorbida,Ivair, AU - Hohenstein,Jessica D, AU - Coates,Brad S, AU - Bevilaqua,Júlia G, AU - Menger,James, AU - Hodgson,Erin W, AU - Koch,Robert L, AU - O'Neal,Matthew E, Y1 - 2022/07/14/ PY - 2021/07/21/received PY - 2021/11/18/accepted PY - 2022/7/14/entrez PY - 2022/7/15/pubmed PY - 2022/7/19/medline SP - 12020 EP - 12020 JF - Scientific reports JO - Sci Rep VL - 12 IS - 1 N2 - The frequent use of insecticides to manage soybean aphids, Aphis glycines (Hemiptera: Aphididae), in the United States has contributed to field-evolved resistance. Pyrethroid-resistant aphids have nonsynonymous mutations in the voltage-gated sodium channel (vgsc). We identified a leucine to phenylalanine mutation at position 1014 (L1014F) and a methionine to isoleucine mutation (M918I) of the A. glycines vgsc, both suspected of conferring knockdown resistance (kdr) to lambda-cyhalothrin. We developed molecular markers to identify these mutations in insecticide-resistant aphids. We determined that A. glycines which survived exposure to a diagnostic concentration of lambda-cyhalothrin and bifenthrin via glass-vial bioassays had these mutations, and showed significant changes in the resistance allele frequency between samples collected before and after field application of lambda-cyhalothrin. Thus, a strong association was revealed between aphids with L1014F and M918I vgsc mutations and survival following exposure to pyrethroids. Specifically, the highest survival was observed for aphids with the kdr (L1014F) and heterozygote super-kdr (L1014F + M918I) genotypes following laboratory bioassays and in-field application of lambda-cyhalothrin. These genetic markers could be used as a diagnostic tool for detecting insecticide-resistant A. glycines and monitoring the geographic distribution of pyrethroid resistance. We discuss how generating these types of data could improve our efforts to mitigate the effects of pyrethroid resistance on crop production. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/35835854/Association_of_voltage_gated_sodium_channel_mutations_with_field_evolved_pyrethroid_resistant_phenotypes_in_soybean_aphid_and_genetic_markers_for_their_detection_ DB - PRIME DP - Unbound Medicine ER -