TY - JOUR T1 - Investigation of Enzymes in the Phthalide Biosynthetic Pathway in Angelica sinensis Using Integrative Metabolite Profiles and Transcriptome Analysis. AU - Feng,Wei-Meng, AU - Liu,Pei, AU - Yan,Hui, AU - Yu,Guang, AU - Zhang,Sen, AU - Jiang,Shu, AU - Shang,Er-Xin, AU - Qian,Da-Wei, AU - Duan,Jin-Ao, Y1 - 2022/07/01/ PY - 2022/04/26/received PY - 2022/06/13/accepted PY - 2022/7/18/entrez PY - 2022/7/19/pubmed PY - 2022/7/19/medline KW - Angelica sinensis KW - phthalides biosynthetic pathway KW - prokaryotic expression KW - regulation mechanism KW - transcriptome SP - 928760 EP - 928760 JF - Frontiers in plant science JO - Front Plant Sci VL - 13 N2 - The roots of Angelica sinensis (Oliv.) Diels are well known for their efficacy in promoting blood circulation. Although many studies have indicated that phthalides are the main chemical components responsible for the pharmacological properties of A. sinensis, the phthalide biosynthetic pathway and enzymes that transform different phthalides are still poorly understood. We identified 108 potential candidate isoforms for phthalide accumulation using transcriptome and metabolite profile analyses. Then, six enzymes, including phospho-2-dehydro-3-deoxyheptonate aldolase 2, shikimate dehydrogenase, primary amine oxidase, polyphenol oxidase, tyrosine decarboxylase, and shikimate O-hydroxycinnamoyl transferase, were identified and proven to be involved in phthalide accumulation by heterologously expressing these proteins in Escherichia coli. We proposed a possible mechanism underlying phthalide transformation and biosynthetic pathways in A. sinensis based on our findings. The results of our study can provide valuable information for understanding the mechanisms underlying phthalide accumulation and transformation and enable further development of quality control during the cultivation of A. sinensis. SN - 1664-462X UR - https://www.unboundmedicine.com/medline/citation/35845641/Investigation_of_Enzymes_in_the_Phthalide_Biosynthetic_Pathway_in_Angelica_sinensis_Using_Integrative_Metabolite_Profiles_and_Transcriptome_Analysis_ DB - PRIME DP - Unbound Medicine ER -