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Integrated LC-MS/MS method and network pharmacology for exploring the characterization and mechanism of neuroprotective effect of Vitis amurensis Rupr. wine polyphenol.
J Food Biochem. 2022 10; 46(10):e14316.JF

Abstract

Polyphenols are the main active components in Vitis amurensis Rupr. wine, which show good protective effects on the nervous system, but their compositions in Vitis amurensis Rupr. wine and the molecular mechanism underlying their neuroprotection remains unclear. The purpose of this study is to investigate the potential mechanism of the neuroprotective effect of Vitis amurensis Rupr. wine polyphenols on the basis of the specific composition of polyphenols in Vitis amurensis Rupr. wine. In this study, 40 phenolic compounds which include 15 anthocyanins, 10 flavonoids, 10 phenolic acids, 3 proanthocyanidins and 2 resveratrols were identified by UPLC Q-Exactive Orbitrap MS. Furthermore, An UPLC-QqQ/MS method was established to simultaneously determine the phenolic compounds in Vitis amurensis Rupr. Wine, and analyze the content differences of phenolic compounds between Vitis amurensis Rupr. and Vitis vinifera wine. Finally, network pharmacology was employed for the first time to predict the possible pharmacological mechanisms of Vitis amurensis wine polyphenols against nervous damage. Multivariate network analysis indicated that quercetin, (-)-epigallocatechin and various anthocyanins were found as prominent compounds for the treatment of nervous system diseases. Vitis amurensis Rupr. wine polyphenols mainly acted on these key targets, including AKT1, EGFR, ESR1, and SRC, and further regulate the PI3K-AKT and Rap1 signaling pathway for treating nervous system diseases. Our findings suggested that polyphenols in Vitis amurensis Rupr. wine possess neuroprotective effect through multicomponent, multitarget, and multichannel. PRACTICAL APPLICATIONS: Studies have revealed that Vitis amurensis Rupr. has higher levels of phenolic compounds than Vitis vinifera wine. However, due to the few and limited study of Vitis amurensis Rupr., their compositions in Vitis amurensis Rupr. wine and the molecular mechanism underlying their neuroprotection remains unclear. This research uses a holistic network pharmacology strategy to investigate the potential targets and pharmacological mechanisms of Vitis amurensis Rupr. wine polyphenols against nervous damage. To some things up, The finding elucidates the relationships between signaling pathways, targets, and compounds in Vitis amurensis Rupr. wine polyphenols, which may provide guidance and foundations for further application of medicinal food.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Zhang Y
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, China.
Yuan D
Jiangsu Haosen Pharmaceutical Group Co., Ltd, Lianyungang, China.
Guo J
School of Functional Food and Wine, Shenyang Pharmaceutical University, Shenyang, China.
Jiang J
School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang, China.
Yao H
School of Functional Food and Wine, Shenyang Pharmaceutical University, Shenyang, China.
Chen Z
School of Functional Food and Wine, Shenyang Pharmaceutical University, Shenyang, China.
Li L
School of Functional Food and Wine, Shenyang Pharmaceutical University, Shenyang, China.
Cui Y
School of Pharmacy, Shenyang Pharmaceutical University, Shenyang, China.

MeSH

AnthocyaninsChromatography, LiquidErbB ReceptorsFlavonoidsNetwork PharmacologyNeuroprotective AgentsPhenolsPhosphatidylinositol 3-KinasesPolyphenolsProanthocyanidinsProto-Oncogene Proteins c-aktQuercetinTandem Mass SpectrometryVitisWine

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

35848530

Citation

Zhang, Ying, et al. "Integrated LC-MS/MS Method and Network Pharmacology for Exploring the Characterization and Mechanism of Neuroprotective Effect of Vitis Amurensis Rupr. Wine Polyphenol." Journal of Food Biochemistry, vol. 46, no. 10, 2022, pp. e14316.
Zhang Y, Yuan D, Guo J, et al. Integrated LC-MS/MS method and network pharmacology for exploring the characterization and mechanism of neuroprotective effect of Vitis amurensis Rupr. wine polyphenol. J Food Biochem. 2022;46(10):e14316.
Zhang, Y., Yuan, D., Guo, J., Jiang, J., Yao, H., Chen, Z., Li, L., & Cui, Y. (2022). Integrated LC-MS/MS method and network pharmacology for exploring the characterization and mechanism of neuroprotective effect of Vitis amurensis Rupr. wine polyphenol. Journal of Food Biochemistry, 46(10), e14316. https://doi.org/10.1111/jfbc.14316
Zhang Y, et al. Integrated LC-MS/MS Method and Network Pharmacology for Exploring the Characterization and Mechanism of Neuroprotective Effect of Vitis Amurensis Rupr. Wine Polyphenol. J Food Biochem. 2022;46(10):e14316. PubMed PMID: 35848530.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Integrated LC-MS/MS method and network pharmacology for exploring the characterization and mechanism of neuroprotective effect of Vitis amurensis Rupr. wine polyphenol. AU - Zhang,Ying, AU - Yuan,Danbing, AU - Guo,Jianyu, AU - Jiang,Jing, AU - Yao,Haoran, AU - Chen,Zhongling, AU - Li,Lingxi, AU - Cui,Yan, Y1 - 2022/07/18/ PY - 2022/07/05/revised PY - 2022/05/17/received PY - 2022/07/07/accepted PY - 2022/7/19/pubmed PY - 2022/10/13/medline PY - 2022/7/18/entrez KW - Vitis amurensis Rupr. Wine KW - network pharmacology KW - neuroprotective effect KW - polyphenol SP - e14316 EP - e14316 JF - Journal of food biochemistry JO - J Food Biochem VL - 46 IS - 10 N2 - Polyphenols are the main active components in Vitis amurensis Rupr. wine, which show good protective effects on the nervous system, but their compositions in Vitis amurensis Rupr. wine and the molecular mechanism underlying their neuroprotection remains unclear. The purpose of this study is to investigate the potential mechanism of the neuroprotective effect of Vitis amurensis Rupr. wine polyphenols on the basis of the specific composition of polyphenols in Vitis amurensis Rupr. wine. In this study, 40 phenolic compounds which include 15 anthocyanins, 10 flavonoids, 10 phenolic acids, 3 proanthocyanidins and 2 resveratrols were identified by UPLC Q-Exactive Orbitrap MS. Furthermore, An UPLC-QqQ/MS method was established to simultaneously determine the phenolic compounds in Vitis amurensis Rupr. Wine, and analyze the content differences of phenolic compounds between Vitis amurensis Rupr. and Vitis vinifera wine. Finally, network pharmacology was employed for the first time to predict the possible pharmacological mechanisms of Vitis amurensis wine polyphenols against nervous damage. Multivariate network analysis indicated that quercetin, (-)-epigallocatechin and various anthocyanins were found as prominent compounds for the treatment of nervous system diseases. Vitis amurensis Rupr. wine polyphenols mainly acted on these key targets, including AKT1, EGFR, ESR1, and SRC, and further regulate the PI3K-AKT and Rap1 signaling pathway for treating nervous system diseases. Our findings suggested that polyphenols in Vitis amurensis Rupr. wine possess neuroprotective effect through multicomponent, multitarget, and multichannel. PRACTICAL APPLICATIONS: Studies have revealed that Vitis amurensis Rupr. has higher levels of phenolic compounds than Vitis vinifera wine. However, due to the few and limited study of Vitis amurensis Rupr., their compositions in Vitis amurensis Rupr. wine and the molecular mechanism underlying their neuroprotection remains unclear. This research uses a holistic network pharmacology strategy to investigate the potential targets and pharmacological mechanisms of Vitis amurensis Rupr. wine polyphenols against nervous damage. To some things up, The finding elucidates the relationships between signaling pathways, targets, and compounds in Vitis amurensis Rupr. wine polyphenols, which may provide guidance and foundations for further application of medicinal food. SN - 1745-4514 UR - https://www.unboundmedicine.com/medline/citation/35848530/Integrated_LC_MS/MS_method_and_network_pharmacology_for_exploring_the_characterization_and_mechanism_of_neuroprotective_effect_of_Vitis_amurensis_Rupr__wine_polyphenol_ DB - PRIME DP - Unbound Medicine ER -