Tags

Type your tag names separated by a space and hit enter

Hair analysis for beta-blockers and calcium-channel blockers by using liquid chromatography-tandem mass spectrometry as a tool for monitoring adherence to antihypertensive therapy.
Drug Test Anal. 2022 Oct; 14(10):1773-1778.DT

Abstract

Adherence to therapy is the key to a successful therapeutic intervention, especially in cardiovascular diseases in which a lack of adherence may have serious consequences in terms morbidity and/or mortality. In this context, hair analysis can be an excellent tool to monitor adherence to therapy. Indeed, drugs present in blood are incorporated into the hair matrix, where drugs and metabolites can stay unaltered for a long time protected from metabolism and degradation. In the present study, a simple, specific, and sensitive ultra-high performance liquid-chromatography-tandem mass spectrometry (UHPLC-MS/MS) method set up to determine in human hair seven beta-blockers (viz., metoprolol, sotalol, labetalol, atenolol, nebivolol, bisoprolol, and nadolol) and two calcium-channel blockers (lercanidipine and amlodipine), which are widely prescribed to treat medium-to-severe hypertensive conditions. The optimized method was successfully validated in terms of accuracy, repeatability, reproducibility, matrix effect and extraction recovery. Moreover, the applicability of the method was evaluated by analyzing 34 real samples of hair obtained from patients under long-term therapy with calcium channel blockers and beta-blockers.

Authors+Show Affiliations

Unit of Forensic Medicine, Department of Diagnostics and Public Health, University of Verona, Verona, Italy.Unit of Forensic Medicine, Department of Diagnostics and Public Health, University of Verona, Verona, Italy.Unit of Forensic Medicine, Department of Diagnostics and Public Health, University of Verona, Verona, Italy.Unit of Forensic Medicine, Department of Diagnostics and Public Health, University of Verona, Verona, Italy.Unit of Forensic Medicine, Department of Diagnostics and Public Health, University of Verona, Verona, Italy. World-Class Research Center "Digital biodesign and personalized healthcare", Sechenov First Moscow State Medical University, Moscow, Russia.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

35855505

Citation

Taus, Francesco, et al. "Hair Analysis for Beta-blockers and Calcium-channel Blockers By Using Liquid Chromatography-tandem Mass Spectrometry as a Tool for Monitoring Adherence to Antihypertensive Therapy." Drug Testing and Analysis, vol. 14, no. 10, 2022, pp. 1773-1778.
Taus F, Gottardo R, Ballotari M, et al. Hair analysis for beta-blockers and calcium-channel blockers by using liquid chromatography-tandem mass spectrometry as a tool for monitoring adherence to antihypertensive therapy. Drug Test Anal. 2022;14(10):1773-1778.
Taus, F., Gottardo, R., Ballotari, M., Utzeri, C., & Tagliaro, F. (2022). Hair analysis for beta-blockers and calcium-channel blockers by using liquid chromatography-tandem mass spectrometry as a tool for monitoring adherence to antihypertensive therapy. Drug Testing and Analysis, 14(10), 1773-1778. https://doi.org/10.1002/dta.3346
Taus F, et al. Hair Analysis for Beta-blockers and Calcium-channel Blockers By Using Liquid Chromatography-tandem Mass Spectrometry as a Tool for Monitoring Adherence to Antihypertensive Therapy. Drug Test Anal. 2022;14(10):1773-1778. PubMed PMID: 35855505.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hair analysis for beta-blockers and calcium-channel blockers by using liquid chromatography-tandem mass spectrometry as a tool for monitoring adherence to antihypertensive therapy. AU - Taus,Francesco, AU - Gottardo,Rossella, AU - Ballotari,Marco, AU - Utzeri,Chiara, AU - Tagliaro,Franco, Y1 - 2022/07/21/ PY - 2022/07/07/revised PY - 2022/03/16/received PY - 2022/07/11/accepted PY - 2022/7/21/pubmed PY - 2022/10/12/medline PY - 2022/7/20/entrez KW - adherence to therapy KW - beta-blockers KW - calcium channel-blockers KW - hair analysis KW - mass spectrometry SP - 1773 EP - 1778 JF - Drug testing and analysis JO - Drug Test Anal VL - 14 IS - 10 N2 - Adherence to therapy is the key to a successful therapeutic intervention, especially in cardiovascular diseases in which a lack of adherence may have serious consequences in terms morbidity and/or mortality. In this context, hair analysis can be an excellent tool to monitor adherence to therapy. Indeed, drugs present in blood are incorporated into the hair matrix, where drugs and metabolites can stay unaltered for a long time protected from metabolism and degradation. In the present study, a simple, specific, and sensitive ultra-high performance liquid-chromatography-tandem mass spectrometry (UHPLC-MS/MS) method set up to determine in human hair seven beta-blockers (viz., metoprolol, sotalol, labetalol, atenolol, nebivolol, bisoprolol, and nadolol) and two calcium-channel blockers (lercanidipine and amlodipine), which are widely prescribed to treat medium-to-severe hypertensive conditions. The optimized method was successfully validated in terms of accuracy, repeatability, reproducibility, matrix effect and extraction recovery. Moreover, the applicability of the method was evaluated by analyzing 34 real samples of hair obtained from patients under long-term therapy with calcium channel blockers and beta-blockers. SN - 1942-7611 UR - https://www.unboundmedicine.com/medline/citation/35855505/Hair_analysis_for_beta-blockers_and_calcium-channel_blockers_by_using_liquid_chromatography-tandem_mass_spectrometry_as_a_tool_for_monitoring_adherence_to_antihypertensive_therapy. DB - PRIME DP - Unbound Medicine ER -