TY - JOUR T1 - Genotypic Characterization of Orientia tsutsugamushi Isolated From Acute Encephalitis Syndrome and Acute Febrile Illness Cases in the Gorakhpur Area, Uttar Pradesh, India. AU - Nanaware,Nikita, AU - Desai,Dipen, AU - Banerjee,Anwesha, AU - Zaman,Kamran, AU - Mittal,Mahim, AU - Mittal,Mahima, AU - Kulkarni,Smita, Y1 - 2022/07/05/ PY - 2022/04/01/received PY - 2022/05/23/accepted PY - 2022/7/22/entrez PY - 2022/7/23/pubmed PY - 2022/7/23/medline KW - Acute Encephalitis Syndrome KW - Gorakhpur KW - India KW - Orientia tsutsugamushi KW - dual infection KW - genotypic characterization KW - scrub typhus SP - 910757 EP - 910757 JF - Frontiers in microbiology JO - Front Microbiol VL - 13 N2 - Scrub typhus infections caused by Orientiatsutsugamushi (OT), continue to remain underdiagnosed globally, due to the lack of distinctive symptoms. The elusive nature of the Acute Encephalitis Syndrome (AES) outbreak in Gorakhpur, Uttar Pradesh that claimed numerous pediatric lives was the driving force of this study which involved serological diagnosis (IgM-ELISA), isolation of OT in cell culture, confirmation by PCR, and characterization by Sanger sequencing. In total, 12 out of 36 patients were seropositive, of which 4 were positive by PCR. Upon enrichment in cell culture, additional 3 patients (including two seronegative) were detected positive by PCR. In total, three of these 7 patients were found to be infected with two strains of OT. Taken together, this study for the first time reports the occurrence of dual infections in addition to three circulating OT genotypes (Gilliam, Kato, and Karp-like) and highlights the significance of enriching OT in cell culture systems for efficient molecular detection. SN - 1664-302X UR - https://www.unboundmedicine.com/medline/citation/35865917/Genotypic_Characterization_of_Orientia_tsutsugamushi_Isolated_From_Acute_Encephalitis_Syndrome_and_Acute_Febrile_Illness_Cases_in_the_Gorakhpur_Area_Uttar_Pradesh_India_ DB - PRIME DP - Unbound Medicine ER -