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Biological Evaluation, Phytochemical Screening, and Fabrication of Indigofera Linifolia Leaves Extract-Loaded Nanoparticles.
Molecules. 2022 Jul 23; 27(15)M

Abstract

Indigofera linifolia is a medicinally important plant, and by virtue of its rich phytochemical composition, this plant is widely used as essential component in traditional medication systems. Due to its wide range of medicinal applications, the extract-loaded chitosan (Ext+Ch), extract-loaded PEG (Ext+PEG), and extract-loaded locust bean gum (Ext+LGB) nanoparticles (NPs) were prepared in the present study. The prepared NPs were then evaluated for their antibacterial, antioxidant, and antidiabetic potentials. Antibacterial activities of the crude extract and the synthesized NPs were performed following standard procedures reported in the literature. The antioxidant capabilities of extract and NPs were evaluated using DPPH free radical scavenging assay. The antidiabetic potential of the samples was evaluated against α-amylase and α-glucosidase. Ext+PEG NPs showed more potent antibacterial activity against the selected strains of bacteria with the highest activity against Escherichia coli. The lowest antibacterial potential was observed for Ext+LGB NPs. The Ext+LGB NPs IC50 value of 39 μg/mL was found to be the most potent inhibitor of DPPH free radicals. Ext+LGB NPs showed a greater extent of inhibition against α-glucosidase and α-amylase with an IC50 of 83 and 78 μg/mL, whereas for the standard acarbose the IC50 values recorded against the mentioned enzymes were 69 and 74 μg/mL, respectively. A high concentration of phenolics and flavonoids in the crude extract was confirmed through TPC and TFC tests, HPLC profiling, and GC-MS analysis. It was considered that the observed antibacterial, antidiabetic, and antioxidant potential might be due the presence of these phenolics and flavonoids detected. The plant could thus be considered as a potential candidate to be used as a remedy of the mentioned health complications. However, further research in this regard is needed to isolate the exact responsible compounds of the observed biological potentials exhibited by the crude extract. Further, toxicity and pharmacological evaluations in animal models are also needed to establish the safety or toxicity profile of the plant.

Authors+Show Affiliations

Department of Biochemistry, University of Malakand, Lower Dir, Chakdara 18800, Pakistan.Department of Chemistry, University of Malakand, Lower Dir, Chakdara 18800, Pakistan.Department of Biochemistry, University of Malakand, Lower Dir, Chakdara 18800, Pakistan.Department of Pharmacy, University of Malakand, Lower Dir, Chakdara 18800, Pakistan.Department of Pharmacy, University of Malakand, Lower Dir, Chakdara 18800, Pakistan.Department of Pharmacy, Shaheed Benazir Bhuto University, Dir Upper, Sheringal, 18000, Pakistan.Department of Zoology, Jinnah College for Women, University of Peshawar, Peshawar 182300, Pakistan.Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Najran University, Najran 61441, Saudi Arabia.Department of Clinical Laboratory Sciences, Najran University, Najran 61441, Saudi Arabia.Department of Clinical Laboratory Sciences, Faculty of Applied Medical Sciences, Najran University, P.O. Box 1988, Najran 61441, Saudi Arabia.Department of Pharmaceutical Chemistry, College of Pharmacy, Najran University, Najran 61441, Saudi Arabia.Shanghai Key Laboratory for Molecular Engineering of Chiral Drugs, School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China. Department of Natural Product Chemistry, School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

35897890

Citation

Talha, Muhammad, et al. "Biological Evaluation, Phytochemical Screening, and Fabrication of Indigofera Linifolia Leaves Extract-Loaded Nanoparticles." Molecules (Basel, Switzerland), vol. 27, no. 15, 2022.
Talha M, Islam NU, Zahoor M, et al. Biological Evaluation, Phytochemical Screening, and Fabrication of Indigofera Linifolia Leaves Extract-Loaded Nanoparticles. Molecules. 2022;27(15).
Talha, M., Islam, N. U., Zahoor, M., Sadiq, A., Nawaz, A., Khan, F. A., Gulfam, N., Alshamrani, S. A., Nahari, M. H., Alshahrani, M. A., Mahnashi, M. H., & Hassan, S. S. U. (2022). Biological Evaluation, Phytochemical Screening, and Fabrication of Indigofera Linifolia Leaves Extract-Loaded Nanoparticles. Molecules (Basel, Switzerland), 27(15). https://doi.org/10.3390/molecules27154707
Talha M, et al. Biological Evaluation, Phytochemical Screening, and Fabrication of Indigofera Linifolia Leaves Extract-Loaded Nanoparticles. Molecules. 2022 Jul 23;27(15) PubMed PMID: 35897890.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Biological Evaluation, Phytochemical Screening, and Fabrication of Indigofera Linifolia Leaves Extract-Loaded Nanoparticles. AU - Talha,Muhammad, AU - Islam,Noor Ul, AU - Zahoor,Muhammad, AU - Sadiq,Abdul, AU - Nawaz,Asif, AU - Khan,Farhat Ali, AU - Gulfam,Naila, AU - Alshamrani,Saleh A, AU - Nahari,Mohammed H, AU - Alshahrani,Mohammed Abdulrahman, AU - Mahnashi,Mater H, AU - Hassan,Syed Shams Ul, Y1 - 2022/07/23/ PY - 2022/06/30/received PY - 2022/07/21/revised PY - 2022/07/21/accepted PY - 2022/7/28/entrez PY - 2022/7/29/pubmed PY - 2022/7/30/medline KW - Indigofera linifolia KW - antidiabetic KW - antioxidant KW - nanoparticles JF - Molecules (Basel, Switzerland) JO - Molecules VL - 27 IS - 15 N2 - Indigofera linifolia is a medicinally important plant, and by virtue of its rich phytochemical composition, this plant is widely used as essential component in traditional medication systems. Due to its wide range of medicinal applications, the extract-loaded chitosan (Ext+Ch), extract-loaded PEG (Ext+PEG), and extract-loaded locust bean gum (Ext+LGB) nanoparticles (NPs) were prepared in the present study. The prepared NPs were then evaluated for their antibacterial, antioxidant, and antidiabetic potentials. Antibacterial activities of the crude extract and the synthesized NPs were performed following standard procedures reported in the literature. The antioxidant capabilities of extract and NPs were evaluated using DPPH free radical scavenging assay. The antidiabetic potential of the samples was evaluated against α-amylase and α-glucosidase. Ext+PEG NPs showed more potent antibacterial activity against the selected strains of bacteria with the highest activity against Escherichia coli. The lowest antibacterial potential was observed for Ext+LGB NPs. The Ext+LGB NPs IC50 value of 39 μg/mL was found to be the most potent inhibitor of DPPH free radicals. Ext+LGB NPs showed a greater extent of inhibition against α-glucosidase and α-amylase with an IC50 of 83 and 78 μg/mL, whereas for the standard acarbose the IC50 values recorded against the mentioned enzymes were 69 and 74 μg/mL, respectively. A high concentration of phenolics and flavonoids in the crude extract was confirmed through TPC and TFC tests, HPLC profiling, and GC-MS analysis. It was considered that the observed antibacterial, antidiabetic, and antioxidant potential might be due the presence of these phenolics and flavonoids detected. The plant could thus be considered as a potential candidate to be used as a remedy of the mentioned health complications. However, further research in this regard is needed to isolate the exact responsible compounds of the observed biological potentials exhibited by the crude extract. Further, toxicity and pharmacological evaluations in animal models are also needed to establish the safety or toxicity profile of the plant. SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/35897890/Biological_Evaluation_Phytochemical_Screening_and_Fabrication_of_Indigofera_Linifolia_Leaves_Extract_Loaded_Nanoparticles_ L2 - https://www.mdpi.com/resolver?pii=molecules27154707 DB - PRIME DP - Unbound Medicine ER -