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Establishment and identification of six wheat-Thinopyrum ponticum disomic addition lines derived from partial amphiploid Xiaoyan 7430.
Theor Appl Genet. 2022 Sep; 135(9):3277-3291.TA

Abstract

KEY MESSAGE

Six wheat-Thinopyrum ponticum disomic addition lines derived from partial amphiploid Xiaoyan 7430 were identified using in situ hybridization and SNP microarray, the homoeologous group and stripe rust resistance of each alien chromosome were determined, and Th. ponticum chromosome-specific markers were developed. Xiaoyan 7430 is a significant partial amphiploid, which is used to set up a bridge for transferring valuable genes from Thinopyrum ponticum (Podp.) Barkworth & D.R. Dewey into common wheat. To accelerate the application of these useful genes in enriching the genetic variability of cultivated wheat by chromosome engineering, a complete set of derived addition lines has been created from Xiaoyan 7430. The chromosome composition of each line was characterized by the combination of genomic in situ hybridization and multicolor fluorescence in situ hybridization (mc-FISH), and the homoeology of each alien chromosome was determined by wheat SNP microarray analysis. Addition line WTA55 with alien group-6 chromosome was evaluated resistant to stripe rust isolates at both the seedling and grain-filling stages (Zadoks scale at z.11 and z.73). Diagnostic marker analysis proved that it could carry a novel stripe rust resistance gene derived from Th. ponticum. Furthermore, a FISH probe and 45 molecular markers specific for alien chromosomes were developed based on specific-locus amplified fragment sequencing (SLAF-seq). Of which 27 markers were separately located on single alien chromosome, and some of them could be used to identify the derived translocation lines. This set of addition lines as well as the molecular markers and the FISH probe will promote the introgression of abundant variation from Th. ponticum into wheat in wheat improvement programs.

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Authors+Show Affiliations

Jia H
State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, 100101, China. School of Basic Medical Science, Guangxi Medical University, Nanning, 530021, Guangxi Zhuang Autonomous Region, China.
Feng H
State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, 100101, China. School of Basic Medical Science, Guangxi Medical University, Nanning, 530021, Guangxi Zhuang Autonomous Region, China.
Yang G
State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, 100101, China. University of Chinese Academy of Sciences, Beijing, 100049, China.
Li H
State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, 100101, China.
Fu S
College of Agronomy, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, Sichuan, China.
Li B
State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, 100101, China.
Li Z
State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, 100101, China.
Zheng Q
State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, 100101, China. qzheng@genetics.ac.cn.

MeSH

BasidiomycotaChromosomes, PlantDisease ResistanceGenetic MarkersIn Situ Hybridization, FluorescencePlant DiseasesPoaceaeTriticum

Pub Type(s)

Journal Article

Language

eng

PubMed ID

35916916

Citation

Jia, Hongwei, et al. "Establishment and Identification of Six wheat-Thinopyrum Ponticum Disomic Addition Lines Derived From Partial Amphiploid Xiaoyan 7430." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 135, no. 9, 2022, pp. 3277-3291.
Jia H, Feng H, Yang G, et al. Establishment and identification of six wheat-Thinopyrum ponticum disomic addition lines derived from partial amphiploid Xiaoyan 7430. Theor Appl Genet. 2022;135(9):3277-3291.
Jia, H., Feng, H., Yang, G., Li, H., Fu, S., Li, B., Li, Z., & Zheng, Q. (2022). Establishment and identification of six wheat-Thinopyrum ponticum disomic addition lines derived from partial amphiploid Xiaoyan 7430. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 135(9), 3277-3291. https://doi.org/10.1007/s00122-022-04185-x
Jia H, et al. Establishment and Identification of Six wheat-Thinopyrum Ponticum Disomic Addition Lines Derived From Partial Amphiploid Xiaoyan 7430. Theor Appl Genet. 2022;135(9):3277-3291. PubMed PMID: 35916916.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Establishment and identification of six wheat-Thinopyrum ponticum disomic addition lines derived from partial amphiploid Xiaoyan 7430. AU - Jia,Hongwei, AU - Feng,Hang, AU - Yang,Guotang, AU - Li,Hongwei, AU - Fu,Shulan, AU - Li,Bin, AU - Li,Zhensheng, AU - Zheng,Qi, Y1 - 2022/08/02/ PY - 2022/03/18/received PY - 2022/07/16/accepted PY - 2022/8/3/pubmed PY - 2022/9/21/medline PY - 2022/8/2/entrez SP - 3277 EP - 3291 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 135 IS - 9 N2 - KEY MESSAGE: Six wheat-Thinopyrum ponticum disomic addition lines derived from partial amphiploid Xiaoyan 7430 were identified using in situ hybridization and SNP microarray, the homoeologous group and stripe rust resistance of each alien chromosome were determined, and Th. ponticum chromosome-specific markers were developed. Xiaoyan 7430 is a significant partial amphiploid, which is used to set up a bridge for transferring valuable genes from Thinopyrum ponticum (Podp.) Barkworth & D.R. Dewey into common wheat. To accelerate the application of these useful genes in enriching the genetic variability of cultivated wheat by chromosome engineering, a complete set of derived addition lines has been created from Xiaoyan 7430. The chromosome composition of each line was characterized by the combination of genomic in situ hybridization and multicolor fluorescence in situ hybridization (mc-FISH), and the homoeology of each alien chromosome was determined by wheat SNP microarray analysis. Addition line WTA55 with alien group-6 chromosome was evaluated resistant to stripe rust isolates at both the seedling and grain-filling stages (Zadoks scale at z.11 and z.73). Diagnostic marker analysis proved that it could carry a novel stripe rust resistance gene derived from Th. ponticum. Furthermore, a FISH probe and 45 molecular markers specific for alien chromosomes were developed based on specific-locus amplified fragment sequencing (SLAF-seq). Of which 27 markers were separately located on single alien chromosome, and some of them could be used to identify the derived translocation lines. This set of addition lines as well as the molecular markers and the FISH probe will promote the introgression of abundant variation from Th. ponticum into wheat in wheat improvement programs. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/35916916/Establishment_and_identification_of_six_wheat_Thinopyrum_ponticum_disomic_addition_lines_derived_from_partial_amphiploid_Xiaoyan_7430_ DB - PRIME DP - Unbound Medicine ER -
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