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Ultrastructural immunocytochemical localization of two hydatid fluid antigens (antigen 5 and antigen B) in the brood capsules and protoscoleces of ovine and equine Echinococcus granulosus and E. multilocularis.
Parasitology 1978; 77(2):143-52P

Abstract

The unlabelled antibody method was used in the ultrastructural localization of two hydatid fluid antigens, antigen 5 and antigen B, in brood capsules and protoscoleces of Echinococcus granulosus and E. multilocularis. Antigen 5 was found in the parenchyma cells of the protoscolex and brood capsule wall and to a lesser extent in the walls of the flame cells and collecting ducts of the excretory system and in the surrounding interstitial material. It is suggested that, while some excretion of this antigen may occur from the protoscolex, it could also be liberated into the cystic cavity by degeneration of protoscoleces and parenchymal cells of the brood capsule wall. Antigen B was found mainly in the distal cytoplasm and perinuclear cytoplasm of the tegument anterior to the suckers. It is apparently secreted to the outside and was present in the brood capsule contents; it adheres to the anterior surface and the posterior periodic acid-Schiff (PAS)-positive glycocalyx of the protoscolex and to the inner surface of the brood capsule wall. The protoscolex tegument posterior to the suckers was negative. The parenchyma cells of the protoscolex and brood capsule wall were also positive although the intensity of the reaction product was variable.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

360151

Citation

Davies, C, et al. "Ultrastructural Immunocytochemical Localization of Two Hydatid Fluid Antigens (antigen 5 and Antigen B) in the Brood Capsules and Protoscoleces of Ovine and Equine Echinococcus Granulosus and E. Multilocularis." Parasitology, vol. 77, no. 2, 1978, pp. 143-52.
Davies C, Rickard MD, Bout DT, et al. Ultrastructural immunocytochemical localization of two hydatid fluid antigens (antigen 5 and antigen B) in the brood capsules and protoscoleces of ovine and equine Echinococcus granulosus and E. multilocularis. Parasitology. 1978;77(2):143-52.
Davies, C., Rickard, M. D., Bout, D. T., & Smyth, J. D. (1978). Ultrastructural immunocytochemical localization of two hydatid fluid antigens (antigen 5 and antigen B) in the brood capsules and protoscoleces of ovine and equine Echinococcus granulosus and E. multilocularis. Parasitology, 77(2), pp. 143-52.
Davies C, et al. Ultrastructural Immunocytochemical Localization of Two Hydatid Fluid Antigens (antigen 5 and Antigen B) in the Brood Capsules and Protoscoleces of Ovine and Equine Echinococcus Granulosus and E. Multilocularis. Parasitology. 1978;77(2):143-52. PubMed PMID: 360151.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ultrastructural immunocytochemical localization of two hydatid fluid antigens (antigen 5 and antigen B) in the brood capsules and protoscoleces of ovine and equine Echinococcus granulosus and E. multilocularis. AU - Davies,C, AU - Rickard,M D, AU - Bout,D T, AU - Smyth,J D, PY - 1978/10/1/pubmed PY - 1978/10/1/medline PY - 1978/10/1/entrez SP - 143 EP - 52 JF - Parasitology JO - Parasitology VL - 77 IS - 2 N2 - The unlabelled antibody method was used in the ultrastructural localization of two hydatid fluid antigens, antigen 5 and antigen B, in brood capsules and protoscoleces of Echinococcus granulosus and E. multilocularis. Antigen 5 was found in the parenchyma cells of the protoscolex and brood capsule wall and to a lesser extent in the walls of the flame cells and collecting ducts of the excretory system and in the surrounding interstitial material. It is suggested that, while some excretion of this antigen may occur from the protoscolex, it could also be liberated into the cystic cavity by degeneration of protoscoleces and parenchymal cells of the brood capsule wall. Antigen B was found mainly in the distal cytoplasm and perinuclear cytoplasm of the tegument anterior to the suckers. It is apparently secreted to the outside and was present in the brood capsule contents; it adheres to the anterior surface and the posterior periodic acid-Schiff (PAS)-positive glycocalyx of the protoscolex and to the inner surface of the brood capsule wall. The protoscolex tegument posterior to the suckers was negative. The parenchyma cells of the protoscolex and brood capsule wall were also positive although the intensity of the reaction product was variable. SN - 0031-1820 UR - https://www.unboundmedicine.com/medline/citation/360151/Ultrastructural_immunocytochemical_localization_of_two_hydatid_fluid_antigens__antigen_5_and_antigen_B__in_the_brood_capsules_and_protoscoleces_of_ovine_and_equine_Echinococcus_granulosus_and_E__multilocularis_ DB - PRIME DP - Unbound Medicine ER -