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Caenorhabditis elegans NHR-14/HNF4α regulates DNA damage-induced apoptosis through cooperating with cep-1/p53.
Cell Commun Signal. 2022 09 01; 20(1):135.CC

Abstract

BACKGROUND

Nuclear hormone receptors are involved in transcriptional regulation and many important cellular processes including development and metabolism. However, its role in DNA damage-induced apoptosis remains elusive.

METHODS

Synchronized young adult animals were irradiated with different doses of gamma-Ray, and then put back to culture at 20 °C. Germline cell apoptosis was scored at different time point.

RESULTS

Deletion of nhr-14 led to decreased DNA damage-induced germline apoptosis, but not the physiological programmed cell death. We also demonstrate that nhr-14 functions downstream of the DNA damage checkpoint pathway. Moreover, we show that nhr-14 regulates egl-1 and ced-13 transcription upon DNA damage. Mechanistically, NHR-14 forms a complex with CEP-1/p53 and binds directly to the egl-1 promoter to promote egl-1 transcription..

CONCLUSIONS

Our results indicate that NHR-14/HNF4α cooperates with CEP-1/p53 to regulate DNA damage-induced apoptosis. Video abstract.

Links

PMC Free PDF

Authors+Show Affiliations

Sang L
Center for Life Sciences, School of Life Sciences, State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming, China.
Dong R
Center for Life Sciences, School of Life Sciences, State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming, China.
Liu R
The Third Affiliated Hospital of Kunming Medical University, Kunming, China.
Hao Q
Center for Life Sciences, School of Life Sciences, State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming, China.
Bai W
Center for Life Sciences, School of Life Sciences, State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming, China.
Sun J
Center for Life Sciences, School of Life Sciences, State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming, China. jwsun@ynu.edu.cn.

MeSH

AnimalsApoptosisCaenorhabditis elegansCaenorhabditis elegans ProteinsDNA DamageTumor Suppressor Protein p53

Pub Type(s)

Journal Article
Video-Audio Media
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

36050770

Citation

Sang, Lei, et al. "Caenorhabditis Elegans NHR-14/HNF4α Regulates DNA Damage-induced Apoptosis Through Cooperating With Cep-1/p53." Cell Communication and Signaling : CCS, vol. 20, no. 1, 2022, p. 135.
Sang L, Dong R, Liu R, et al. Caenorhabditis elegans NHR-14/HNF4α regulates DNA damage-induced apoptosis through cooperating with cep-1/p53. Cell Commun Signal. 2022;20(1):135.
Sang, L., Dong, R., Liu, R., Hao, Q., Bai, W., & Sun, J. (2022). Caenorhabditis elegans NHR-14/HNF4α regulates DNA damage-induced apoptosis through cooperating with cep-1/p53. Cell Communication and Signaling : CCS, 20(1), 135. https://doi.org/10.1186/s12964-022-00920-5
Sang L, et al. Caenorhabditis Elegans NHR-14/HNF4α Regulates DNA Damage-induced Apoptosis Through Cooperating With Cep-1/p53. Cell Commun Signal. 2022 09 1;20(1):135. PubMed PMID: 36050770.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Caenorhabditis elegans NHR-14/HNF4α regulates DNA damage-induced apoptosis through cooperating with cep-1/p53. AU - Sang,Lei, AU - Dong,Rui, AU - Liu,Rui, AU - Hao,Qinggang, AU - Bai,Weiyu, AU - Sun,Jianwei, Y1 - 2022/09/01/ PY - 2022/01/09/received PY - 2022/06/16/accepted PY - 2022/9/1/entrez PY - 2022/9/2/pubmed PY - 2022/9/9/medline KW - Apoptosis KW - CEP-1/p53 KW - Caenorhabditis elegans KW - DNA damage KW - NHR-14 SP - 135 EP - 135 JF - Cell communication and signaling : CCS JO - Cell Commun Signal VL - 20 IS - 1 N2 - BACKGROUND: Nuclear hormone receptors are involved in transcriptional regulation and many important cellular processes including development and metabolism. However, its role in DNA damage-induced apoptosis remains elusive. METHODS: Synchronized young adult animals were irradiated with different doses of gamma-Ray, and then put back to culture at 20 °C. Germline cell apoptosis was scored at different time point. RESULTS: Deletion of nhr-14 led to decreased DNA damage-induced germline apoptosis, but not the physiological programmed cell death. We also demonstrate that nhr-14 functions downstream of the DNA damage checkpoint pathway. Moreover, we show that nhr-14 regulates egl-1 and ced-13 transcription upon DNA damage. Mechanistically, NHR-14 forms a complex with CEP-1/p53 and binds directly to the egl-1 promoter to promote egl-1 transcription.. CONCLUSIONS: Our results indicate that NHR-14/HNF4α cooperates with CEP-1/p53 to regulate DNA damage-induced apoptosis. Video abstract. SN - 1478-811X UR - https://www.unboundmedicine.com/medline/citation/36050770/Caenorhabditis_elegans_NHR_14/HNF4α_regulates_DNA_damage_induced_apoptosis_through_cooperating_with_cep_1/p53_ DB - PRIME DP - Unbound Medicine ER -