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Bovine pancreatic trypsin inhibitor and soybean Kunitz trypsin inhibitor: Differential effects on proteases and larval development of the soybean pest Anticarsia gemmatalis (Lepidoptera: Noctuidae).
Pestic Biochem Physiol. 2022 Oct; 187:105188.PB

Abstract

Pest management is challenged with resistant herbivores and problems regarding human health and environmental issues. Indeed, the greatest challenge to modern agriculture is to protect crops from pests and still maintain environmental quality. This study aimed to analyze by in silico, in vitro, and in vivo approaches to the feasibility of using the inhibitory protein extracted from mammals - Bovine Pancreatic Trypsin Inhibitor (BPTI) as a potential inhibitor of digestive trypsins from the pest Anticarsia gemmatalis and comparing the results with the host-plant inhibitor - Soybean Kunitz Trypsin Inhibitor (SKTI). BPTI and SKTI interacts with A. gemmatalis trypsin-like enzyme competitively, through hydrogen and hydrophobic bonds. A. gemmatalis larvae exposed to BPTI did not show two common adaptative mechanisms i.e., proteolytic degradation and overproduction of proteases, presenting highly reduced trypsin-like activity. On the other hand, SKTI-fed larvae did not show reduced trypsin-like activity, presenting overproduction of proteases and SKTI digestion. In addition, the larval survival was reduced by BPTI similarly to SKTI, and additionally caused a decrease in pupal weight. The non-plant protease inhibitor BPTI presents intriguing element to compose biopesticide formulations to help decrease the use of conventional refractory pesticides into integrated pest management programs.

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Authors+Show Affiliations

de Almeida Barros R
Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Minas Gerais, Brazil; Instituto de Biotecnologia aplicada à Agropecuária, BIOAGRO-UFV, Viçosa, Minas Gerais, Brazil.
Meriño-Cabrera Y
Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Minas Gerais, Brazil; Instituto de Biotecnologia aplicada à Agropecuária, BIOAGRO-UFV, Viçosa, Minas Gerais, Brazil.
Castro JS
Departamento de Física, Universidad de Sucre, Sincelejo, Colombia; Instituto de Biotecnologia aplicada à Agropecuária, BIOAGRO-UFV, Viçosa, Minas Gerais, Brazil.
da Silva Junior NR
Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Minas Gerais, Brazil; Instituto de Biotecnologia aplicada à Agropecuária, BIOAGRO-UFV, Viçosa, Minas Gerais, Brazil.
de Oliveira JVA
Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Minas Gerais, Brazil; Instituto de Biotecnologia aplicada à Agropecuária, BIOAGRO-UFV, Viçosa, Minas Gerais, Brazil.
Schultz H
Departamento de Entomologia, Universidade Federal de Viçosa, Minas Gerais, Brazil; Instituto de Biotecnologia aplicada à Agropecuária, BIOAGRO-UFV, Viçosa, Minas Gerais, Brazil.
de Andrade RJ
Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Minas Gerais, Brazil; Instituto de Biotecnologia aplicada à Agropecuária, BIOAGRO-UFV, Viçosa, Minas Gerais, Brazil.
de Oliveira Ramos HJ
Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Minas Gerais, Brazil; Instituto de Biotecnologia aplicada à Agropecuária, BIOAGRO-UFV, Viçosa, Minas Gerais, Brazil.
de Almeida Oliveira MG
Departamento de Bioquímica e Biologia Molecular, Universidade Federal de Viçosa, Minas Gerais, Brazil; Instituto de Biotecnologia aplicada à Agropecuária, BIOAGRO-UFV, Viçosa, Minas Gerais, Brazil. Electronic address: malmeida@ufv.br.

MeSH

AnimalsAprotininBiological Control AgentsCattleHydrogenLarvaMothsPeptide HydrolasesPesticidesProtease InhibitorsSoybeansTrypsinTrypsin Inhibitors

Pub Type(s)

Journal Article

Language

eng

PubMed ID

36127063

Citation

de Almeida Barros, Rafael, et al. "Bovine Pancreatic Trypsin Inhibitor and Soybean Kunitz Trypsin Inhibitor: Differential Effects On Proteases and Larval Development of the Soybean Pest Anticarsia Gemmatalis (Lepidoptera: Noctuidae)." Pesticide Biochemistry and Physiology, vol. 187, 2022, p. 105188.
de Almeida Barros R, Meriño-Cabrera Y, Castro JS, et al. Bovine pancreatic trypsin inhibitor and soybean Kunitz trypsin inhibitor: Differential effects on proteases and larval development of the soybean pest Anticarsia gemmatalis (Lepidoptera: Noctuidae). Pestic Biochem Physiol. 2022;187:105188.
de Almeida Barros, R., Meriño-Cabrera, Y., Castro, J. S., da Silva Junior, N. R., de Oliveira, J. V. A., Schultz, H., de Andrade, R. J., de Oliveira Ramos, H. J., & de Almeida Oliveira, M. G. (2022). Bovine pancreatic trypsin inhibitor and soybean Kunitz trypsin inhibitor: Differential effects on proteases and larval development of the soybean pest Anticarsia gemmatalis (Lepidoptera: Noctuidae). Pesticide Biochemistry and Physiology, 187, 105188. https://doi.org/10.1016/j.pestbp.2022.105188
de Almeida Barros R, et al. Bovine Pancreatic Trypsin Inhibitor and Soybean Kunitz Trypsin Inhibitor: Differential Effects On Proteases and Larval Development of the Soybean Pest Anticarsia Gemmatalis (Lepidoptera: Noctuidae). Pestic Biochem Physiol. 2022;187:105188. PubMed PMID: 36127063.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bovine pancreatic trypsin inhibitor and soybean Kunitz trypsin inhibitor: Differential effects on proteases and larval development of the soybean pest Anticarsia gemmatalis (Lepidoptera: Noctuidae). AU - de Almeida Barros,Rafael, AU - Meriño-Cabrera,Yaremis, AU - Castro,José Severiche, AU - da Silva Junior,Neilier Rodrigues, AU - de Oliveira,João Vitor Aguilar, AU - Schultz,Halina, AU - de Andrade,Rafael Júnior, AU - de Oliveira Ramos,Humberto Josué, AU - de Almeida Oliveira,Maria Goreti, Y1 - 2022/08/01/ PY - 2022/05/18/received PY - 2022/07/26/revised PY - 2022/07/27/accepted PY - 2022/9/20/entrez PY - 2022/9/21/pubmed PY - 2022/9/24/medline KW - Adaptative responses KW - Electrophoresis KW - Enzyme kinetics KW - Inhibition KW - Molecular dynamics KW - Protease inhibitors SP - 105188 EP - 105188 JF - Pesticide biochemistry and physiology JO - Pestic Biochem Physiol VL - 187 N2 - Pest management is challenged with resistant herbivores and problems regarding human health and environmental issues. Indeed, the greatest challenge to modern agriculture is to protect crops from pests and still maintain environmental quality. This study aimed to analyze by in silico, in vitro, and in vivo approaches to the feasibility of using the inhibitory protein extracted from mammals - Bovine Pancreatic Trypsin Inhibitor (BPTI) as a potential inhibitor of digestive trypsins from the pest Anticarsia gemmatalis and comparing the results with the host-plant inhibitor - Soybean Kunitz Trypsin Inhibitor (SKTI). BPTI and SKTI interacts with A. gemmatalis trypsin-like enzyme competitively, through hydrogen and hydrophobic bonds. A. gemmatalis larvae exposed to BPTI did not show two common adaptative mechanisms i.e., proteolytic degradation and overproduction of proteases, presenting highly reduced trypsin-like activity. On the other hand, SKTI-fed larvae did not show reduced trypsin-like activity, presenting overproduction of proteases and SKTI digestion. In addition, the larval survival was reduced by BPTI similarly to SKTI, and additionally caused a decrease in pupal weight. The non-plant protease inhibitor BPTI presents intriguing element to compose biopesticide formulations to help decrease the use of conventional refractory pesticides into integrated pest management programs. SN - 1095-9939 UR - https://www.unboundmedicine.com/medline/citation/36127063/Bovine_pancreatic_trypsin_inhibitor_and_soybean_Kunitz_trypsin_inhibitor:_Differential_effects_on_proteases_and_larval_development_of_the_soybean_pest_Anticarsia_gemmatalis__Lepidoptera:_Noctuidae__ DB - PRIME DP - Unbound Medicine ER -