Tags

Type your tag names separated by a space and hit enter

Up-regulation of miR-133a-3p promotes ovary insulin resistance on granulosa cells of obese PCOS patients via inhibiting PI3K/AKT signaling.
BMC Womens Health. 2022 10 08; 22(1):412.BW

Abstract

BACKGROUND

MicroRNAs are a type of non-coding single-stranded RNA, which is involved in the regulation of ovary insulin resistance (IR). This study aims to explore the underlying mechanisms of miR-133a-3p regulating ovary IR in obese polycystic ovary syndrome (PCOS).

METHODS

Granulosa cells (GCs) were extracted from follicular fluids of PCOS patients (obese PCOS group and non-obese PCOS group) and healthy women (control group). The expression of miR-133a-3p in GCs was detected by qRT-PCR. The targets and pathways of miR-133a-3p were predicted by bioinformatics analyses. The protein levels of PI3K, p-AKT, GLUT4, p-GSK-3β, and p-FOXO1 were measured by Western blotting.

RESULTS

MiR-133a-3p was highly expressed in GCs from PCOS patients, especially in obese PCOS patients. The protein levels of PI3K and p-AKT was downregulated in GCs from PCOS patients. There were 11 target genes of miR-133a-3p enriching in PI3K/AKT signaling pathway. miR-133a-3p mimic downregulated the expression of PI3K, p-AKT, and GLUT4, and upregulated the protein levels of p-GSK-3β and p-FOXO1. miR-133a-3p inhibitor presented the opposite effect of miR-133a-3p mimic.

CONCLUSION

MiR-133a-3p promotes ovary IR on GCs of obese PCOS patients via inhibiting PI3K/AKT signaling pathway. This study lays a foundation for further research on the mechanism of ovary IR in obese PCOS patients.

Links

Authors+Show Affiliations

Shandong University of Traditional Chinese Medicine, Jinan, China.

Integrative Medicine Center for Reproductive and Heredity, The Affiliated Hospital of Shandong University of Traditional Chinese Medicine, No. 42 Wenhuaxi Road, Jinan, China. wkh2021@163.com.

Shandong University of Traditional Chinese Medicine, Jinan, China.

Shandong University of Traditional Chinese Medicine, Jinan, China.

Integrative Medicine Center for Reproductive and Heredity, The Affiliated Hospital of Shandong University of Traditional Chinese Medicine, No. 42 Wenhuaxi Road, Jinan, China.

Integrative Medicine Center for Reproductive and Heredity, The Affiliated Hospital of Shandong University of Traditional Chinese Medicine, No. 42 Wenhuaxi Road, Jinan, China.

Shandong University of Traditional Chinese Medicine, Jinan, China.

Shandong University of Traditional Chinese Medicine, Jinan, China.

Shandong University of Traditional Chinese Medicine, Jinan, China.

Shandong University of Traditional Chinese Medicine, Jinan, China.

Shandong University of Traditional Chinese Medicine, Jinan, China.

Shandong University of Traditional Chinese Medicine, Jinan, China.

MeSH

FemaleGlycogen Synthase Kinase 3 betaGranulosa CellsHumansInsulin ResistanceMicroRNAsPhosphatidylinositol 3-KinasesPolycystic Ovary SyndromeProto-Oncogene Proteins c-aktSignal TransductionUp-Regulation

Pub Type(s)

Journal Article

Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

36209087

Citation

Yang, Xiaoman, et al. "Up-regulation of miR-133a-3p Promotes Ovary Insulin Resistance On Granulosa Cells of Obese PCOS Patients Via Inhibiting PI3K/AKT Signaling." BMC Women's Health, vol. 22, no. 1, 2022, p. 412.

Yang X, Wang K, Lang J, et al. Up-regulation of miR-133a-3p promotes ovary insulin resistance on granulosa cells of obese PCOS patients via inhibiting PI3K/AKT signaling. BMC Womens Health. 2022;22(1):412.

Yang, X., Wang, K., Lang, J., Guo, D., Gao, H., Qiu, Y., Jin, X., Zhang, M., Shi, J., Ma, Q., Ma, Q., & Wen, Z. (2022). Up-regulation of miR-133a-3p promotes ovary insulin resistance on granulosa cells of obese PCOS patients via inhibiting PI3K/AKT signaling. BMC Women's Health, 22(1), 412. https://doi.org/10.1186/s12905-022-01994-6

Yang X, et al. Up-regulation of miR-133a-3p Promotes Ovary Insulin Resistance On Granulosa Cells of Obese PCOS Patients Via Inhibiting PI3K/AKT Signaling. BMC Womens Health. 2022 10 8;22(1):412. PubMed PMID: 36209087.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Up-regulation of miR-133a-3p promotes ovary insulin resistance on granulosa cells of obese PCOS patients via inhibiting PI3K/AKT signaling. AU - Yang,Xiaoman, AU - Wang,Kehua, AU - Lang,Jiajia, AU - Guo,Danyang, AU - Gao,Haixia, AU - Qiu,Yue, AU - Jin,Xiaohan, AU - Zhang,Mingyue, AU - Shi,Jiaxiu, AU - Ma,QianQian, AU - Ma,Qian, AU - Wen,Zixi, Y1 - 2022/10/08/ PY - 2021/10/21/received PY - 2022/09/26/accepted PY - 2022/10/8/entrez PY - 2022/10/9/pubmed PY - 2022/10/12/medline KW - Granulosa cells KW - Insulin resistance KW - Obesity KW - PI3K/AKT signaling KW - Polycystic ovarian syndrome KW - miR-133a-3p SP - 412 EP - 412 JF - BMC women's health JO - BMC Womens Health VL - 22 IS - 1 N2 - BACKGROUND: MicroRNAs are a type of non-coding single-stranded RNA, which is involved in the regulation of ovary insulin resistance (IR). This study aims to explore the underlying mechanisms of miR-133a-3p regulating ovary IR in obese polycystic ovary syndrome (PCOS). METHODS: Granulosa cells (GCs) were extracted from follicular fluids of PCOS patients (obese PCOS group and non-obese PCOS group) and healthy women (control group). The expression of miR-133a-3p in GCs was detected by qRT-PCR. The targets and pathways of miR-133a-3p were predicted by bioinformatics analyses. The protein levels of PI3K, p-AKT, GLUT4, p-GSK-3β, and p-FOXO1 were measured by Western blotting. RESULTS: MiR-133a-3p was highly expressed in GCs from PCOS patients, especially in obese PCOS patients. The protein levels of PI3K and p-AKT was downregulated in GCs from PCOS patients. There were 11 target genes of miR-133a-3p enriching in PI3K/AKT signaling pathway. miR-133a-3p mimic downregulated the expression of PI3K, p-AKT, and GLUT4, and upregulated the protein levels of p-GSK-3β and p-FOXO1. miR-133a-3p inhibitor presented the opposite effect of miR-133a-3p mimic. CONCLUSION: MiR-133a-3p promotes ovary IR on GCs of obese PCOS patients via inhibiting PI3K/AKT signaling pathway. This study lays a foundation for further research on the mechanism of ovary IR in obese PCOS patients. SN - 1472-6874 UR - https://www.unboundmedicine.com/medline/citation/36209087/Up_regulation_of_miR_133a_3p_promotes_ovary_insulin_resistance_on_granulosa_cells_of_obese_PCOS_patients_via_inhibiting_PI3K/AKT_signaling_ DB - PRIME DP - Unbound Medicine ER -