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Diode-array spectrophotometry for simultaneous measurement of hemoglobin pigments.
Clin Chim Acta. 1987 Jul 15; 166(2-3):283-9.CC

Abstract

A prototype oxygen saturation meter was used to measure the concentrations of deoxygenated hemoglobin (rHb), oxyhemoglobin (HbO2), carboxyhemoglobin (HbCO), methemoglobin (MetHb), and sulfhemoglobin (SHb) in 35 microliter blood. Simultaneous absorbance measurements at 535, 560, 577, 622, 636, and 670 nm permitted the composition of any hemoglobin pigment mixture to be determined more accurately, precisely and easily than before. The inclusion of 670 nm, where the hemoglobin pigments have low absorption coefficients, allowed correction for turbidity.

Authors

Fogh-Andersen N
No affiliation info available
Siggaard-Andersen O
No affiliation info available
Lundsgaard FC
No affiliation info available
Wimberley PD
No affiliation info available

MeSH

AdultCarboxyhemoglobinChromatography, GasHemoglobinsHumansMethemoglobinNephelometry and TurbidimetryOxygenOxyhemoglobinsSpectrophotometrySulfhemoglobin

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

3621605

Citation

Fogh-Andersen, N, et al. "Diode-array Spectrophotometry for Simultaneous Measurement of Hemoglobin Pigments." Clinica Chimica Acta; International Journal of Clinical Chemistry, vol. 166, no. 2-3, 1987, pp. 283-9.
Fogh-Andersen N, Siggaard-Andersen O, Lundsgaard FC, et al. Diode-array spectrophotometry for simultaneous measurement of hemoglobin pigments. Clin Chim Acta. 1987;166(2-3):283-9.
Fogh-Andersen, N., Siggaard-Andersen, O., Lundsgaard, F. C., & Wimberley, P. D. (1987). Diode-array spectrophotometry for simultaneous measurement of hemoglobin pigments. Clinica Chimica Acta; International Journal of Clinical Chemistry, 166(2-3), 283-9.
Fogh-Andersen N, et al. Diode-array Spectrophotometry for Simultaneous Measurement of Hemoglobin Pigments. Clin Chim Acta. 1987 Jul 15;166(2-3):283-9. PubMed PMID: 3621605.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diode-array spectrophotometry for simultaneous measurement of hemoglobin pigments. AU - Fogh-Andersen,N, AU - Siggaard-Andersen,O, AU - Lundsgaard,F C, AU - Wimberley,P D, PY - 1987/7/15/pubmed PY - 1987/7/15/medline PY - 1987/7/15/entrez SP - 283 EP - 9 JF - Clinica chimica acta; international journal of clinical chemistry JO - Clin Chim Acta VL - 166 IS - 2-3 N2 - A prototype oxygen saturation meter was used to measure the concentrations of deoxygenated hemoglobin (rHb), oxyhemoglobin (HbO2), carboxyhemoglobin (HbCO), methemoglobin (MetHb), and sulfhemoglobin (SHb) in 35 microliter blood. Simultaneous absorbance measurements at 535, 560, 577, 622, 636, and 670 nm permitted the composition of any hemoglobin pigment mixture to be determined more accurately, precisely and easily than before. The inclusion of 670 nm, where the hemoglobin pigments have low absorption coefficients, allowed correction for turbidity. SN - 0009-8981 UR - https://www.unboundmedicine.com/medline/citation/3621605/Diode_array_spectrophotometry_for_simultaneous_measurement_of_hemoglobin_pigments_ DB - PRIME DP - Unbound Medicine ER -