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Orthologous genes Pm12 and Pm21 from two wild relatives of wheat show evolutionary conservation but divergent powdery mildew resistance.
Plant Commun. 2023 03 13; 4(2):100472.PC

Abstract

Wheat powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a devastating disease that threatens wheat production worldwide. Pm12, which originated from Aegilops speltoides, a wild relative of wheat, confers strong resistance to powdery mildew and therefore has potential use in wheat breeding. Using susceptible mutants induced by gamma irradiation, we physically mapped and isolated Pm12 and showed it to be orthologous to Pm21 from Dasypyrum villosum, also a wild relative of wheat. The resistance function of Pm12 was validated via ethyl methanesulfonate mutagenesis, virus-induced gene silencing, and stable genetic transformation. Evolutionary analysis indicates that the Pm12/Pm21 loci in wheat species are relatively conserved but dynamic. Here, we demonstrated that the two orthologous genes, Pm12 and Pm21, possess differential resistance against the same set of Bgt isolates. Overexpression of the coiled-coil domains of both PM12 and PM21 induces cell death in Nicotiana benthamiana leaves. However, their full-length forms display different cell death-inducing activities caused by their distinct intramolecular interactions. Cloning of Pm12 will facilitate its application in wheat breeding programs. This study also gives new insight into two orthologous resistance genes, Pm12 and Pm21, which show different race specificities and intramolecular interaction patterns.

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Authors+Show Affiliations

School of Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China; School of Life Sciences, Jiangsu University, Zhenjiang 212013, China.

Crop Research Institution, Shandong Academy of Agricultural Sciences, Jinan 250100, China.

Institute of Plant Protection and Soil Science, Hubei Academy of Agricultural Sciences, Wuhan 430064, China.

School of Life Sciences, Jiangsu University, Zhenjiang 212013, China.

School of Life Sciences, Henan University, Kaifeng 475004, China.

School of Life Sciences, Jiangsu University, Zhenjiang 212013, China.

School of Life Sciences, Jiangsu University, Zhenjiang 212013, China.

School of Life Sciences, Jiangsu University, Zhenjiang 212013, China.

School of Life Sciences, Jiangsu University, Zhenjiang 212013, China.

Crop Research Institution, Shandong Academy of Agricultural Sciences, Jinan 250100, China.

State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing 100101, China.

State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing 100101, China.

State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing 100101, China.

State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing 100101, China.

School of Life Sciences, Henan University, Kaifeng 475004, China. Electronic address: algao@henu.edu.cn.

College of Life Sciences, Yantai University, Yantai 264005, China. Electronic address: ptma@ytu.edu.cn.

School of Life Sciences, Jiangsu University, Zhenjiang 212013, China. Electronic address: hghe@ujs.edu.cn.

MeSH

TriticumPlant BreedingGenes, PlantPoaceae

Pub Type(s)

Journal Article

Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

36352792

Citation

Zhu, Shanying, et al. "Orthologous Genes Pm12 and Pm21 From Two Wild Relatives of Wheat Show Evolutionary Conservation but Divergent Powdery Mildew Resistance." Plant Communications, vol. 4, no. 2, 2023, p. 100472.

Zhu S, Liu C, Gong S, et al. Orthologous genes Pm12 and Pm21 from two wild relatives of wheat show evolutionary conservation but divergent powdery mildew resistance. Plant Commun. 2023;4(2):100472.

Zhu, S., Liu, C., Gong, S., Chen, Z., Chen, R., Liu, T., Liu, R., Du, H., Guo, R., Li, G., Li, M., Fan, R., Liu, Z., Shen, Q. H., Gao, A., Ma, P., & He, H. (2023). Orthologous genes Pm12 and Pm21 from two wild relatives of wheat show evolutionary conservation but divergent powdery mildew resistance. Plant Communications, 4(2), 100472. https://doi.org/10.1016/j.xplc.2022.100472

Zhu S, et al. Orthologous Genes Pm12 and Pm21 From Two Wild Relatives of Wheat Show Evolutionary Conservation but Divergent Powdery Mildew Resistance. Plant Commun. 2023 03 13;4(2):100472. PubMed PMID: 36352792.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Orthologous genes Pm12 and Pm21 from two wild relatives of wheat show evolutionary conservation but divergent powdery mildew resistance. AU - Zhu,Shanying, AU - Liu,Cheng, AU - Gong,Shuangjun, AU - Chen,Zhaozhao, AU - Chen,Rong, AU - Liu,Tianlei, AU - Liu,Renkang, AU - Du,Haonan, AU - Guo,Rui, AU - Li,Genying, AU - Li,Miaomiao, AU - Fan,Renchun, AU - Liu,Zhiyong, AU - Shen,Qian-Hua, AU - Gao,Anli, AU - Ma,Pengtao, AU - He,Huagang, Y1 - 2022/11/09/ PY - 2022/07/18/received PY - 2022/09/23/revised PY - 2022/11/07/accepted PY - 2022/11/11/pubmed PY - 2023/3/17/medline PY - 2022/11/10/entrez KW - Pm12 KW - Pm21 KW - evolutionary conservation KW - intramolecular interaction KW - powdery mildew resistance KW - race specificity SP - 100472 EP - 100472 JF - Plant communications JO - Plant Commun VL - 4 IS - 2 N2 - Wheat powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a devastating disease that threatens wheat production worldwide. Pm12, which originated from Aegilops speltoides, a wild relative of wheat, confers strong resistance to powdery mildew and therefore has potential use in wheat breeding. Using susceptible mutants induced by gamma irradiation, we physically mapped and isolated Pm12 and showed it to be orthologous to Pm21 from Dasypyrum villosum, also a wild relative of wheat. The resistance function of Pm12 was validated via ethyl methanesulfonate mutagenesis, virus-induced gene silencing, and stable genetic transformation. Evolutionary analysis indicates that the Pm12/Pm21 loci in wheat species are relatively conserved but dynamic. Here, we demonstrated that the two orthologous genes, Pm12 and Pm21, possess differential resistance against the same set of Bgt isolates. Overexpression of the coiled-coil domains of both PM12 and PM21 induces cell death in Nicotiana benthamiana leaves. However, their full-length forms display different cell death-inducing activities caused by their distinct intramolecular interactions. Cloning of Pm12 will facilitate its application in wheat breeding programs. This study also gives new insight into two orthologous resistance genes, Pm12 and Pm21, which show different race specificities and intramolecular interaction patterns. SN - 2590-3462 UR - https://www.unboundmedicine.com/medline/citation/36352792/Orthologous_genes_Pm12_and_Pm21_from_two_wild_relatives_of_wheat_show_evolutionary_conservation_but_divergent_powdery_mildew_resistance_ DB - PRIME DP - Unbound Medicine ER -