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Genome-Wide Identification and Characterization of YUCCA Gene Family in Mikania micrantha.
Int J Mol Sci. 2022 Oct 27; 23(21)IJ

Abstract

Auxin is a general coordinator for growth and development throughout plant lifespan, acting in a concentration-dependent manner. Tryptophan aminotransferases (YUCCA) family catalyze the oxidative decarboxylation of indole-3-pyruvic acid (IPA) to form indole-3-acetic acid (IAA) and plays a critical role in auxin homeostasis. Here, 18 YUCCA family genes divided into four categories were identified from Mikania micrantha (M. micrantha), one of the world's most invasive plants. Five highly conserved motifs were characterized in these YUCCA genes (MmYUCs). Transcriptome analysis revealed that MmYUCs exhibited distinct expression patterns in different organs and five MmYUCs showed high expression levels throughout all the five tissues, implying that they may play dominant roles in auxin biosynthesis and plant development. In addition, MmYUC6_1 was overexpressed in DR5::GUS Arabidopsis line to explore its function, which resulted in remarkably increased auxin level and typical elevated auxin-related phenotypes including shortened roots and elongated hypocotyls in the transgenic plants, suggesting that MmYUC6_1 promoted IAA biosynthesis in Arabidopsis. Collectively, these findings provided comprehensive insight into the phylogenetic relationships, chromosomal distributions, expression patterns and functions of the MmYUC genes in M. micrantha, which would facilitate the study of molecular mechanisms underlying the fast growth of M. micrantha and preventing its invasion.

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Authors+Show Affiliations

Luo W
Guangdong Provincial Key Laboratory for Plant Epigenetics, Longhua Bioindustry and Innovation Research Institute, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China. College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China.
Xiao N
Guangdong Provincial Key Laboratory for Plant Epigenetics, Longhua Bioindustry and Innovation Research Institute, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China.
Wu F
Guangdong Provincial Key Laboratory for Plant Epigenetics, Longhua Bioindustry and Innovation Research Institute, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China.
Mo B
Guangdong Provincial Key Laboratory for Plant Epigenetics, Longhua Bioindustry and Innovation Research Institute, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China.
Kong W
Guangdong Provincial Key Laboratory for Plant Epigenetics, Longhua Bioindustry and Innovation Research Institute, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China.
Yu Y
Guangdong Provincial Key Laboratory for Plant Epigenetics, Longhua Bioindustry and Innovation Research Institute, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China.

MeSH

ArabidopsisMikaniaYuccaPhylogenyIndoleacetic AcidsArabidopsis ProteinsPlants, Genetically ModifiedGene Expression Regulation, Plant

Pub Type(s)

Journal Article

Language

eng

PubMed ID

36361840

Citation

Luo, Weigui, et al. "Genome-Wide Identification and Characterization of YUCCA Gene Family in Mikania Micrantha." International Journal of Molecular Sciences, vol. 23, no. 21, 2022.
Luo W, Xiao N, Wu F, et al. Genome-Wide Identification and Characterization of YUCCA Gene Family in Mikania micrantha. Int J Mol Sci. 2022;23(21).
Luo, W., Xiao, N., Wu, F., Mo, B., Kong, W., & Yu, Y. (2022). Genome-Wide Identification and Characterization of YUCCA Gene Family in Mikania micrantha. International Journal of Molecular Sciences, 23(21). https://doi.org/10.3390/ijms232113037
Luo W, et al. Genome-Wide Identification and Characterization of YUCCA Gene Family in Mikania Micrantha. Int J Mol Sci. 2022 Oct 27;23(21) PubMed PMID: 36361840.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genome-Wide Identification and Characterization of YUCCA Gene Family in Mikania micrantha. AU - Luo,Weigui, AU - Xiao,Nian, AU - Wu,Feiyan, AU - Mo,Beixin, AU - Kong,Wenwen, AU - Yu,Yu, Y1 - 2022/10/27/ PY - 2022/09/07/received PY - 2022/10/21/revised PY - 2022/10/24/accepted PY - 2022/11/11/entrez PY - 2022/11/12/pubmed PY - 2022/11/15/medline KW - Mikania micrantha KW - YUCCA KW - auxin KW - growth and development JF - International journal of molecular sciences JO - Int J Mol Sci VL - 23 IS - 21 N2 - Auxin is a general coordinator for growth and development throughout plant lifespan, acting in a concentration-dependent manner. Tryptophan aminotransferases (YUCCA) family catalyze the oxidative decarboxylation of indole-3-pyruvic acid (IPA) to form indole-3-acetic acid (IAA) and plays a critical role in auxin homeostasis. Here, 18 YUCCA family genes divided into four categories were identified from Mikania micrantha (M. micrantha), one of the world's most invasive plants. Five highly conserved motifs were characterized in these YUCCA genes (MmYUCs). Transcriptome analysis revealed that MmYUCs exhibited distinct expression patterns in different organs and five MmYUCs showed high expression levels throughout all the five tissues, implying that they may play dominant roles in auxin biosynthesis and plant development. In addition, MmYUC6_1 was overexpressed in DR5::GUS Arabidopsis line to explore its function, which resulted in remarkably increased auxin level and typical elevated auxin-related phenotypes including shortened roots and elongated hypocotyls in the transgenic plants, suggesting that MmYUC6_1 promoted IAA biosynthesis in Arabidopsis. Collectively, these findings provided comprehensive insight into the phylogenetic relationships, chromosomal distributions, expression patterns and functions of the MmYUC genes in M. micrantha, which would facilitate the study of molecular mechanisms underlying the fast growth of M. micrantha and preventing its invasion. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/36361840/Genome_Wide_Identification_and_Characterization_of_YUCCA_Gene_Family_in_Mikania_micrantha_ DB - PRIME DP - Unbound Medicine ER -