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Construction of a hybrid bacteriophage-plasmid recombinant DNA vector.
J Bacteriol. 1978 Dec; 136(3):1192-6.JB

Abstract

A phage-plasmid hybrid was constructed for use as a recombinant DNA vector, allowing the propagation of cloned EcoRI restriction endonuclease fragments of about 2 X 10(6) to 11 X 10(6) daltons. The colicin E1 plasmid replicon was fused to the left arm of a lambdagt generalized transducing phage with a thermolabile repressor, yielding a genome which could be replicated either by phage lambda functions or via the colicin E1 plasmid replicon. At the nonpermissive temperature, phage functions were derepressed and phage growth occurred lytically. Alternatively, at the permissive temperature, lambda functions were repressed and the vector replicated as a covalently closed circular plasmid. The phage-plasmid hybrid vector could be maintained at a copy number determined by the colicin E1 plasmid replicon and was also sensitive to amplification after chloramphenicol treatment. An EcoRI fragment of Escherichia coli DNA encoding genes of the arabinose operon also was inserted into the central portion of the vector.

Authors

No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

363694

Citation

Donoghue, D J., and P A. Sharp. "Construction of a Hybrid Bacteriophage-plasmid Recombinant DNA Vector." Journal of Bacteriology, vol. 136, no. 3, 1978, pp. 1192-6.
Donoghue DJ, Sharp PA. Construction of a hybrid bacteriophage-plasmid recombinant DNA vector. J Bacteriol. 1978;136(3):1192-6.
Donoghue, D. J., & Sharp, P. A. (1978). Construction of a hybrid bacteriophage-plasmid recombinant DNA vector. Journal of Bacteriology, 136(3), 1192-6.
Donoghue DJ, Sharp PA. Construction of a Hybrid Bacteriophage-plasmid Recombinant DNA Vector. J Bacteriol. 1978;136(3):1192-6. PubMed PMID: 363694.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Construction of a hybrid bacteriophage-plasmid recombinant DNA vector. AU - Donoghue,D J, AU - Sharp,P A, PY - 1978/12/1/pubmed PY - 1978/12/1/medline PY - 1978/12/1/entrez SP - 1192 EP - 6 JF - Journal of bacteriology JO - J. Bacteriol. VL - 136 IS - 3 N2 - A phage-plasmid hybrid was constructed for use as a recombinant DNA vector, allowing the propagation of cloned EcoRI restriction endonuclease fragments of about 2 X 10(6) to 11 X 10(6) daltons. The colicin E1 plasmid replicon was fused to the left arm of a lambdagt generalized transducing phage with a thermolabile repressor, yielding a genome which could be replicated either by phage lambda functions or via the colicin E1 plasmid replicon. At the nonpermissive temperature, phage functions were derepressed and phage growth occurred lytically. Alternatively, at the permissive temperature, lambda functions were repressed and the vector replicated as a covalently closed circular plasmid. The phage-plasmid hybrid vector could be maintained at a copy number determined by the colicin E1 plasmid replicon and was also sensitive to amplification after chloramphenicol treatment. An EcoRI fragment of Escherichia coli DNA encoding genes of the arabinose operon also was inserted into the central portion of the vector. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/363694/Construction_of_a_hybrid_bacteriophage_plasmid_recombinant_DNA_vector_ L2 - http://jb.asm.org/cgi/pmidlookup?view=long&pmid=363694 DB - PRIME DP - Unbound Medicine ER -