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Metabolomics profiling of culture medium reveals association of methionine and vitamin B metabolisms with virulent phenotypes of clinical bloodstream-isolated Candida albicans.
Res Microbiol. 2023 Mar-Apr; 174(3):104009.RM

Abstract

Candida albicans is a predominant species causing candidemia in hospitalized patients. This study aimed to investigate the association of culture medium metabolomic profiles with biofilm formation and invasion properties of clinical bloodstream-isolated C. albicans. A total of twelve isolates and two reference strains were identified by virulent phenotypes. Their susceptibility was determined by the microdilution method, following EUCAST guidelines. Biofilm formation was evaluated with metabolic activity, morphology and agglutinin-like sequence 3 (ALS3) mRNA expression. Invasion into the vascular endothelial EA.hy926 cells was determined by lactate dehydrogenase release and internalization assay. Their metabolomic profiles were assessed by high-resolution accurate-mass spectrometry (HRAMS). The results showed four different phenotypes of C. albicans: high-biofilm/invasive (50%), high-biofilm/non-invasive (7%), low-biofilm/invasive (36%) and low-biofilm/non-invasive (7%). The metabolomic profiles of the culture medium determined strong correlation of the virulent phenotypes and the alteration of metabolites in the methionine metabolism pathway, such as homocysteine, 5-methyltetrahydrofolate and S-adenosylmethioninamine. Moreover, thiamine and biotin levels were significantly increased in Isolate03, representative of a high-biofilm/invasive phenotype. These results suggest that methionine and vitamin B metabolism pathways might be influenced by their virulent phenotypes and pathogenic traits. Therefore, their metabolism pathways might be a potential target for reducing virulence of C. albicans bloodstream infections.

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Authors+Show Affiliations

Kanchanapiboon J
Medicinal Plant Research Institute, Department of Medical Sciences, Ministry of Public Health, Nonthaburi 11000, Thailand. Electronic address: jamras.k@dmsc.mail.go.th.
Maiuthed A
Department of Pharmacology, Faculty of Pharmacy, Mahidol University, Bangkok 10400, Thailand; Centre of Biopharmaceutical Science for Healthy Ageing, Faculty of Pharmacy, Mahidol University, Bangkok 10400, Thailand. Electronic address: arnatchai.mai@mahidol.ac.th.
Rukthong P
Department of Pharmaceutical Technology, Faculty of Pharmacy, Srinakharinwirot University, Nakornnayok 26120, Thailand; Center for Excellence in Plant and Herbal Innovation Research, Strategic Wisdom and Research Institute, Srinakharinwirot University, Nakornnayok 26120, Thailand. Electronic address: pattarawit@g.swu.ac.th.
Thunyaharn S
Department of Medical Technology, Faculty of Allied Health Sciences, Nakhonratchasima College, Nakhon Ratchasima 30000, Thailand. Electronic address: tanmicro@nmc.ac.th.
Tuntoaw S
Medicinal Plant Research Institute, Department of Medical Sciences, Ministry of Public Health, Nonthaburi 11000, Thailand. Electronic address: sasiwan.t@dmsc.mail.go.th.
Poonsatha S
Medicinal Plant Research Institute, Department of Medical Sciences, Ministry of Public Health, Nonthaburi 11000, Thailand. Electronic address: subhadhcha.p@dmsc.mail.go.th.
Santimaleeworagun W
Department of Pharmacy, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom 73000, Thailand. Electronic address: swichai1234@gmail.com.

MeSH

Candida albicansMethionineCandidemiaPhenotypeRacemethionineVitaminsBiofilmsAntifungal Agents

Pub Type(s)

Journal Article

Language

eng

PubMed ID

36403754

Citation

Kanchanapiboon, Jamras, et al. "Metabolomics Profiling of Culture Medium Reveals Association of Methionine and Vitamin B Metabolisms With Virulent Phenotypes of Clinical Bloodstream-isolated Candida Albicans." Research in Microbiology, vol. 174, no. 3, 2023, p. 104009.
Kanchanapiboon J, Maiuthed A, Rukthong P, et al. Metabolomics profiling of culture medium reveals association of methionine and vitamin B metabolisms with virulent phenotypes of clinical bloodstream-isolated Candida albicans. Res Microbiol. 2023;174(3):104009.
Kanchanapiboon, J., Maiuthed, A., Rukthong, P., Thunyaharn, S., Tuntoaw, S., Poonsatha, S., & Santimaleeworagun, W. (2023). Metabolomics profiling of culture medium reveals association of methionine and vitamin B metabolisms with virulent phenotypes of clinical bloodstream-isolated Candida albicans. Research in Microbiology, 174(3), 104009. https://doi.org/10.1016/j.resmic.2022.104009
Kanchanapiboon J, et al. Metabolomics Profiling of Culture Medium Reveals Association of Methionine and Vitamin B Metabolisms With Virulent Phenotypes of Clinical Bloodstream-isolated Candida Albicans. Res Microbiol. 2023;174(3):104009. PubMed PMID: 36403754.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Metabolomics profiling of culture medium reveals association of methionine and vitamin B metabolisms with virulent phenotypes of clinical bloodstream-isolated Candida albicans. AU - Kanchanapiboon,Jamras, AU - Maiuthed,Arnatchai, AU - Rukthong,Pattarawit, AU - Thunyaharn,Sudaluck, AU - Tuntoaw,Sasiwan, AU - Poonsatha,Subhadhcha, AU - Santimaleeworagun,Wichai, Y1 - 2022/11/17/ PY - 2022/7/25/received PY - 2022/11/10/revised PY - 2022/11/10/accepted PY - 2022/11/21/pubmed PY - 2023/2/9/medline PY - 2022/11/20/entrez KW - Biofilm formation KW - Candida albicans KW - Host–pathogen interaction KW - Invasion KW - Metabolomic profiles KW - Virulence SP - 104009 EP - 104009 JF - Research in microbiology JO - Res Microbiol VL - 174 IS - 3 N2 - Candida albicans is a predominant species causing candidemia in hospitalized patients. This study aimed to investigate the association of culture medium metabolomic profiles with biofilm formation and invasion properties of clinical bloodstream-isolated C. albicans. A total of twelve isolates and two reference strains were identified by virulent phenotypes. Their susceptibility was determined by the microdilution method, following EUCAST guidelines. Biofilm formation was evaluated with metabolic activity, morphology and agglutinin-like sequence 3 (ALS3) mRNA expression. Invasion into the vascular endothelial EA.hy926 cells was determined by lactate dehydrogenase release and internalization assay. Their metabolomic profiles were assessed by high-resolution accurate-mass spectrometry (HRAMS). The results showed four different phenotypes of C. albicans: high-biofilm/invasive (50%), high-biofilm/non-invasive (7%), low-biofilm/invasive (36%) and low-biofilm/non-invasive (7%). The metabolomic profiles of the culture medium determined strong correlation of the virulent phenotypes and the alteration of metabolites in the methionine metabolism pathway, such as homocysteine, 5-methyltetrahydrofolate and S-adenosylmethioninamine. Moreover, thiamine and biotin levels were significantly increased in Isolate03, representative of a high-biofilm/invasive phenotype. These results suggest that methionine and vitamin B metabolism pathways might be influenced by their virulent phenotypes and pathogenic traits. Therefore, their metabolism pathways might be a potential target for reducing virulence of C. albicans bloodstream infections. SN - 1769-7123 UR - https://www.unboundmedicine.com/medline/citation/36403754/Metabolomics_profiling_of_culture_medium_reveals_association_of_methionine_and_vitamin_B_metabolisms_with_virulent_phenotypes_of_clinical_bloodstream_isolated_Candida_albicans_ DB - PRIME DP - Unbound Medicine ER -