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Effect of mutations at the lariat branch acceptor site on beta-globin pre-mRNA splicing in vitro.
Nature. 1986 Dec 11-17; 324(6097):589-91.Nat

Abstract

Introns are excised from full-length transcripts (pre-messenger RNAs) of eukaryotic genes in two steps. First, the pre-mRNA is cleaved at the 5' splice site and a branched (lariat) intermediate is formed. Then, cleavage at the 3' splice site and ligation of the two exons leads to the release of the lariat intron. The intron sequence which accepts the 5' end to form the lariat branch is strictly conserved in yeast, but shows more variation in eukaryotes. To investigate the requirements for branch formation in eukaryotes further, we have studied in vitro splicing of a rabbit globin gene intron with mutations of the normal branch-accepting adenosine nucleotide. We conclude that all four nucleotides can serve as branch acceptors, but that A and C are preferred to G and U in lariat formation. Mutation of the normal A to G or U can lead to an A residue one nucleotide upstream of the normal branch site being used instead. Only branches to A or C participate efficiently in the second splicing step.

Authors

No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

3641062

Citation

Hornig, H, et al. "Effect of Mutations at the Lariat Branch Acceptor Site On Beta-globin pre-mRNA Splicing in Vitro." Nature, vol. 324, no. 6097, 1986, pp. 589-91.
Hornig H, Aebi M, Weissmann C. Effect of mutations at the lariat branch acceptor site on beta-globin pre-mRNA splicing in vitro. Nature. 1986;324(6097):589-91.
Hornig, H., Aebi, M., & Weissmann, C. (1986). Effect of mutations at the lariat branch acceptor site on beta-globin pre-mRNA splicing in vitro. Nature, 324(6097), 589-91.
Hornig H, Aebi M, Weissmann C. Effect of Mutations at the Lariat Branch Acceptor Site On Beta-globin pre-mRNA Splicing in Vitro. Nature. 1986 Dec 11-17;324(6097):589-91. PubMed PMID: 3641062.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of mutations at the lariat branch acceptor site on beta-globin pre-mRNA splicing in vitro. AU - Hornig,H, AU - Aebi,M, AU - Weissmann,C, PY - 1986/12/11/pubmed PY - 1986/12/11/medline PY - 1986/12/11/entrez SP - 589 EP - 91 JF - Nature JO - Nature VL - 324 IS - 6097 N2 - Introns are excised from full-length transcripts (pre-messenger RNAs) of eukaryotic genes in two steps. First, the pre-mRNA is cleaved at the 5' splice site and a branched (lariat) intermediate is formed. Then, cleavage at the 3' splice site and ligation of the two exons leads to the release of the lariat intron. The intron sequence which accepts the 5' end to form the lariat branch is strictly conserved in yeast, but shows more variation in eukaryotes. To investigate the requirements for branch formation in eukaryotes further, we have studied in vitro splicing of a rabbit globin gene intron with mutations of the normal branch-accepting adenosine nucleotide. We conclude that all four nucleotides can serve as branch acceptors, but that A and C are preferred to G and U in lariat formation. Mutation of the normal A to G or U can lead to an A residue one nucleotide upstream of the normal branch site being used instead. Only branches to A or C participate efficiently in the second splicing step. SN - 0028-0836 UR - https://www.unboundmedicine.com/medline/citation/3641062/Effect_of_mutations_at_the_lariat_branch_acceptor_site_on_beta_globin_pre_mRNA_splicing_in_vitro_ L2 - https://doi.org/10.1038/324589a0 DB - PRIME DP - Unbound Medicine ER -