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Improvement in the Sequential Extraction of Phycobiliproteins from Arthrospira platensis Using Green Technologies.
Life (Basel). 2022 Nov 15; 12(11)L

Abstract

Arthrospira platensis (commercially known as Spirulina) is an excellent source of phycobiliproteins, especially C-phycocyanin. Phycobiliproteins are significant bioactive compounds with useful biological applications. The extraction process plays a significant role in downstream microalga production and utilisation. The important pigments found in A. platensis include chlorophyll and carotenoids as nonpolar pigments and phycobiliproteins as polar pigments. Supercritical fluid extraction (SFE) as a green extraction technology for the high-value metabolites of microalgae has potential for trends in food and human health. The nonpolar bioactive compounds, chlorophyll and carotenoids of A. platensis, were primarily separated using supercritical carbon dioxide (SC-CO2) solvent-free fluid extraction pressure; the temperature and ethanol as cosolvent conditions were compared. The residue from the A. platensis cells was subjected to phycobiliprotein extraction. The phosphate and water extraction of A. platensis SFE residue were compared to evaluate phycobiliprotein extraction. The SFE results exhibited higher pressure (350 bar) and temperature extraction (50 °C) with ethanol-free extraction and increased nonpolar pigment. Phycobiliprotein yield was obtained from A. platensis SFE residue by ethanol-free buffer extraction as a suitable process with antioxidant properties. The C-phycocyanin was isolated and enhanced to 0.7 purity as food grade. This developed method can be used as a guideline and applied as a sustainable process for important pigment extraction from Arthrospira microalgae.

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Authors+Show Affiliations

Pan-Utai W
Department of Applied Microbiology, Institute of Food Research and Product Development, Kasetsart University, Chatuchak, Bangkok 10900, Thailand.
Iamtham S
Department of Science, Faculty of Liberal Arts and Science, Kasetsart University, Kamphaeng Saen, Nakhon Pathom 73140, Thailand. Center for Agricultural Biotechnology, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand. Center of Excellence on Agricultural Biotechnology-(AG-BIO/PERDO-CHE), Bangkok 10900, Thailand.
Boonbumrung S
Department of Food Chemistry and Physics, Institute of Food Research and Product Development, Kasetsart University, Chatuchak, Bangkok 10900, Thailand.
Mookdasanit J
Department of Fishery Products, Faculty of Fisheries, Kasetsart University, Chatuchak, Bangkok 10900, Thailand.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

36431030

Citation

Pan-Utai, Wanida, et al. "Improvement in the Sequential Extraction of Phycobiliproteins From Arthrospira Platensis Using Green Technologies." Life (Basel, Switzerland), vol. 12, no. 11, 2022.
Pan-Utai W, Iamtham S, Boonbumrung S, et al. Improvement in the Sequential Extraction of Phycobiliproteins from Arthrospira platensis Using Green Technologies. Life (Basel). 2022;12(11).
Pan-Utai, W., Iamtham, S., Boonbumrung, S., & Mookdasanit, J. (2022). Improvement in the Sequential Extraction of Phycobiliproteins from Arthrospira platensis Using Green Technologies. Life (Basel, Switzerland), 12(11). https://doi.org/10.3390/life12111896
Pan-Utai W, et al. Improvement in the Sequential Extraction of Phycobiliproteins From Arthrospira Platensis Using Green Technologies. Life (Basel). 2022 Nov 15;12(11) PubMed PMID: 36431030.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Improvement in the Sequential Extraction of Phycobiliproteins from Arthrospira platensis Using Green Technologies. AU - Pan-Utai,Wanida, AU - Iamtham,Siriluck, AU - Boonbumrung,Sumitra, AU - Mookdasanit,Juta, Y1 - 2022/11/15/ PY - 2022/10/20/received PY - 2022/11/10/revised PY - 2022/11/12/accepted PY - 2022/11/26/entrez PY - 2022/11/27/pubmed PY - 2022/11/27/medline KW - Arthrospira KW - Spirulina KW - green technology KW - phycobiliproteins KW - pigments KW - supercritical fluid extraction JF - Life (Basel, Switzerland) JO - Life (Basel) VL - 12 IS - 11 N2 - Arthrospira platensis (commercially known as Spirulina) is an excellent source of phycobiliproteins, especially C-phycocyanin. Phycobiliproteins are significant bioactive compounds with useful biological applications. The extraction process plays a significant role in downstream microalga production and utilisation. The important pigments found in A. platensis include chlorophyll and carotenoids as nonpolar pigments and phycobiliproteins as polar pigments. Supercritical fluid extraction (SFE) as a green extraction technology for the high-value metabolites of microalgae has potential for trends in food and human health. The nonpolar bioactive compounds, chlorophyll and carotenoids of A. platensis, were primarily separated using supercritical carbon dioxide (SC-CO2) solvent-free fluid extraction pressure; the temperature and ethanol as cosolvent conditions were compared. The residue from the A. platensis cells was subjected to phycobiliprotein extraction. The phosphate and water extraction of A. platensis SFE residue were compared to evaluate phycobiliprotein extraction. The SFE results exhibited higher pressure (350 bar) and temperature extraction (50 °C) with ethanol-free extraction and increased nonpolar pigment. Phycobiliprotein yield was obtained from A. platensis SFE residue by ethanol-free buffer extraction as a suitable process with antioxidant properties. The C-phycocyanin was isolated and enhanced to 0.7 purity as food grade. This developed method can be used as a guideline and applied as a sustainable process for important pigment extraction from Arthrospira microalgae. SN - 2075-1729 UR - https://www.unboundmedicine.com/medline/citation/36431030/Improvement_in_the_Sequential_Extraction_of_Phycobiliproteins_from_Arthrospira_platensis_Using_Green_Technologies_ DB - PRIME DP - Unbound Medicine ER -