Tags

Type your tag names separated by a space and hit enter

Genome-wide association study for resistance in bread wheat (Triticum aestivum L.) to stripe rust (Puccinia striiformis f. sp. tritici) races in Argentina.
BMC Plant Biol. 2022 Nov 24; 22(1):543.BP

Abstract

BACKGROUND

Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most devastating diseases of the wheat crop. It causes significant reductions in both grain yield and grain quality. In recent years, new and more virulent races have overcome many of the known resistance genes in Argentinian germplasm. In order to identify loci conferring resistance to the local races of Pst for effective utilization in future breeding programs, a genome-wide association study (GWAS) was performed using a collection of 245 bread wheat lines genotyped with 90 K SNPs.

RESULTS

To search for adult plant resistance (APR) the panel was evaluated for disease severity (DS) and area under disease progress curve (AUDPC) in field trials during two years under natural infection conditions. To look for seedling or all-stage resistance (ASR) the panel was evaluated to determine infection type (IT) under greenhouse conditions against two prevalent races in Argentina. The phenotypic data showed that the panel possessed enough genetic variability for searching for sources of resistance to Pst. Significant correlations between years were observed for Pst response in the field and high heritability values were found for DS (H2 = 0.89) and AUDPC (H2 = 0.93). Based on GWAS, eight markers associated with Pst resistance (FDR < 0.01) were identified, of these, five were associated with ASR (on chromosomes 1B, 2A, 3A and 5B) and three with APR (on chromosomes 3B and 7A). These markers explained between 2% and 32.62% of the phenotypic variation. Five of the markers corresponded with previously reported Yr genes/QTL, while the other three (QYr.Bce.1B.sd.1, QYr.Bce.3A.sd and QYr.Bce.3B.APR.2) might be novel resistance loci.

CONCLUSION

Our results revealed high genetic variation for resistance to Argentinian stripe rust races in the germplasm used here. It constitutes a very promising step towards the improvement of Pst resistance of bread wheat in Argentina. Also, the identification of new resistance loci would represent a substantial advance for diversifying the current set of resistance genes and to advance in the improvement of the durable resistance to the disease.

Links

PMC Free PDF

Authors+Show Affiliations

Franco MF
Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata, 7620, Balcarce, CP, Argentina. franco.fiorella@inta.gob.ar. Consejo Nacional de Investigaciones Científicas Y Técnicas (CONICET), Buenos Aires, Argentina. franco.fiorella@inta.gob.ar. Estación Experimental Agropecuaria INTA Balcarce, 7620, Balcarce, CP, Argentina. franco.fiorella@inta.gob.ar.
Polacco AN
Facultad de Ciencias Agrarias, Universidad Nacional de Mar del Plata, 7620, Balcarce, CP, Argentina.
Campos PE
Estación Experimental Agropecuaria INTA Bordenave, 8187, Bordenave, CP, Argentina.
Pontaroli AC
Consejo Nacional de Investigaciones Científicas Y Técnicas (CONICET), Buenos Aires, Argentina. Estación Experimental Agropecuaria INTA Balcarce, 7620, Balcarce, CP, Argentina.
Vanzetti LS
Consejo Nacional de Investigaciones Científicas Y Técnicas (CONICET), Buenos Aires, Argentina. Estación Experimental Agropecuaria INTA Marcos Juárez, 2580, Marcos Juárez, CP, Argentina.

MeSH

TriticumGenome-Wide Association StudyBreadDisease ResistanceQuantitative Trait LociArgentinaPlant DiseasesPlant BreedingBasidiomycota

Pub Type(s)

Journal Article

Language

eng

PubMed ID

36434507

Citation

Franco, M F., et al. "Genome-wide Association Study for Resistance in Bread Wheat (Triticum Aestivum L.) to Stripe Rust (Puccinia Striiformis F. Sp. Tritici) Races in Argentina." BMC Plant Biology, vol. 22, no. 1, 2022, p. 543.
Franco MF, Polacco AN, Campos PE, et al. Genome-wide association study for resistance in bread wheat (Triticum aestivum L.) to stripe rust (Puccinia striiformis f. sp. tritici) races in Argentina. BMC Plant Biol. 2022;22(1):543.
Franco, M. F., Polacco, A. N., Campos, P. E., Pontaroli, A. C., & Vanzetti, L. S. (2022). Genome-wide association study for resistance in bread wheat (Triticum aestivum L.) to stripe rust (Puccinia striiformis f. sp. tritici) races in Argentina. BMC Plant Biology, 22(1), 543. https://doi.org/10.1186/s12870-022-03916-y
Franco MF, et al. Genome-wide Association Study for Resistance in Bread Wheat (Triticum Aestivum L.) to Stripe Rust (Puccinia Striiformis F. Sp. Tritici) Races in Argentina. BMC Plant Biol. 2022 Nov 24;22(1):543. PubMed PMID: 36434507.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genome-wide association study for resistance in bread wheat (Triticum aestivum L.) to stripe rust (Puccinia striiformis f. sp. tritici) races in Argentina. AU - Franco,M F, AU - Polacco,A N, AU - Campos,P E, AU - Pontaroli,A C, AU - Vanzetti,L S, Y1 - 2022/11/24/ PY - 2022/08/29/received PY - 2022/10/27/accepted PY - 2022/11/26/entrez PY - 2022/11/27/pubmed PY - 2022/11/30/medline KW - Adult plant resistance (APR) KW - All-stage resistance (ASR) KW - Genome-wide association study (GWAS) KW - Single nucleotide polymorphism (SNP) SP - 543 EP - 543 JF - BMC plant biology JO - BMC Plant Biol VL - 22 IS - 1 N2 - BACKGROUND: Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most devastating diseases of the wheat crop. It causes significant reductions in both grain yield and grain quality. In recent years, new and more virulent races have overcome many of the known resistance genes in Argentinian germplasm. In order to identify loci conferring resistance to the local races of Pst for effective utilization in future breeding programs, a genome-wide association study (GWAS) was performed using a collection of 245 bread wheat lines genotyped with 90 K SNPs. RESULTS: To search for adult plant resistance (APR) the panel was evaluated for disease severity (DS) and area under disease progress curve (AUDPC) in field trials during two years under natural infection conditions. To look for seedling or all-stage resistance (ASR) the panel was evaluated to determine infection type (IT) under greenhouse conditions against two prevalent races in Argentina. The phenotypic data showed that the panel possessed enough genetic variability for searching for sources of resistance to Pst. Significant correlations between years were observed for Pst response in the field and high heritability values were found for DS (H2 = 0.89) and AUDPC (H2 = 0.93). Based on GWAS, eight markers associated with Pst resistance (FDR < 0.01) were identified, of these, five were associated with ASR (on chromosomes 1B, 2A, 3A and 5B) and three with APR (on chromosomes 3B and 7A). These markers explained between 2% and 32.62% of the phenotypic variation. Five of the markers corresponded with previously reported Yr genes/QTL, while the other three (QYr.Bce.1B.sd.1, QYr.Bce.3A.sd and QYr.Bce.3B.APR.2) might be novel resistance loci. CONCLUSION: Our results revealed high genetic variation for resistance to Argentinian stripe rust races in the germplasm used here. It constitutes a very promising step towards the improvement of Pst resistance of bread wheat in Argentina. Also, the identification of new resistance loci would represent a substantial advance for diversifying the current set of resistance genes and to advance in the improvement of the durable resistance to the disease. SN - 1471-2229 UR - https://www.unboundmedicine.com/medline/citation/36434507/Genome_wide_association_study_for_resistance_in_bread_wheat__Triticum_aestivum_L___to_stripe_rust__Puccinia_striiformis_f__sp__tritici__races_in_Argentina_ DB - PRIME DP - Unbound Medicine ER -