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Simultaneous determination and quality evaluation of 16 compounds in Ligustri Lucidi Fructus covering different regions and processed products using ultra-high-performance liquid chromatography-mass spectrometry.
Biomed Chromatogr. 2023 Mar; 37(3):e5564.BC

Abstract

A quantitative analysis method and a chemical pattern recognition method were developed to evaluate raw Ligustri Lucidi Fructus (LLF) from different regions and different processed products. In this study, a comprehensive strategy using ultra-high-performance liquid chromatography-mass spectrometry quantitative analysis method was established for the simultaneous determination of 16 components in 47 batches of LLF covering 19 regions belonging to 8 provinces and 24 batches of different processed products (steamed LLF without auxiliary material, wine-steamed LLF, salt-steamed LLF, and vinegar-steamed LLF). The results of this study indicated that the proposed method was reliable and accurate for the rapid analysis proved by detection limit, quantification limit, precision, and accuracy. Furthermore, principal component analysis and hierarchical cluster analysis were employed to analyze the experimental data, showing that the best-quality samples of 47 batches of raw LLF were S47 (Lantian, Shaanxi), S39 (Pingyang-2, Shandong), S38 (Pingyang-1, Shandong), and S45 (Lingbao, Henan), whereas the worst-quality samples were S7-S16 (Huzhou, Zhejiang). In 24 batches of processed products, the best-quality samples were S48 (salt steamed 2 h), S60 (wine steamed 2 h), and S61 (wine steamed 4 h). Meanwhile, the heat map showed that the contents of triterpenoid saponins, including C16 (ursolic acid), C15 (oleanic acid), and C14 (maslinic acid), were higher than those of other compounds in 71 batches of samples. These results suggested that the quality of raw LLF in the central and northern regions was better than that in the southern regions, and regarding the processed products, different auxiliary materials had little effect on the quality of LLF, but steaming time of 2 h was appropriate. Briefly, this study proposed a multiparameter quantitative analysis method for the overall quality control of raw LLF samples covering different regions in China and different processed LLF.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Li X
Department of Resources and Identification of Chinese Medicine, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, National Key Laboratory Breeding Base of Systematic Research, Development and Utilization of Chinese Medicine Resources, Chengdu, P. R. China. Ankang Inspection and Testing Center for Food and Drug, Ankang, P. R. China.
Jia J
Shaanxi Institute for Food and Drug Control, Shaanxi Key Laboratory of Food and Drug Safety Monitoring, Xi'an, P. R. China.
Li T
Shaanxi Institute for Food and Drug Control, Shaanxi Key Laboratory of Food and Drug Safety Monitoring, Xi'an, P. R. China.
Zhao Z
Shaanxi University of Chinese Medicine, Xianyang, P. R. China.
Ren C
Department of Resources and Identification of Chinese Medicine, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, National Key Laboratory Breeding Base of Systematic Research, Development and Utilization of Chinese Medicine Resources, Chengdu, P. R. China.
Liu H
Shaanxi Institute for Food and Drug Control, Shaanxi Key Laboratory of Food and Drug Safety Monitoring, Xi'an, P. R. China.
Pei J
Department of Resources and Identification of Chinese Medicine, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, National Key Laboratory Breeding Base of Systematic Research, Development and Utilization of Chinese Medicine Resources, Chengdu, P. R. China.

MeSH

Drugs, Chinese HerbalLigustrumChromatography, High Pressure LiquidMass SpectrometryQuality ControlSodium Chloride

Pub Type(s)

Journal Article

Language

eng

PubMed ID

36509695

Citation

Li, Xiaoan, et al. "Simultaneous Determination and Quality Evaluation of 16 Compounds in Ligustri Lucidi Fructus Covering Different Regions and Processed Products Using Ultra-high-performance Liquid Chromatography-mass Spectrometry." Biomedical Chromatography : BMC, vol. 37, no. 3, 2023, pp. e5564.
Li X, Jia J, Li T, et al. Simultaneous determination and quality evaluation of 16 compounds in Ligustri Lucidi Fructus covering different regions and processed products using ultra-high-performance liquid chromatography-mass spectrometry. Biomed Chromatogr. 2023;37(3):e5564.
Li, X., Jia, J., Li, T., Zhao, Z., Ren, C., Liu, H., & Pei, J. (2023). Simultaneous determination and quality evaluation of 16 compounds in Ligustri Lucidi Fructus covering different regions and processed products using ultra-high-performance liquid chromatography-mass spectrometry. Biomedical Chromatography : BMC, 37(3), e5564. https://doi.org/10.1002/bmc.5564
Li X, et al. Simultaneous Determination and Quality Evaluation of 16 Compounds in Ligustri Lucidi Fructus Covering Different Regions and Processed Products Using Ultra-high-performance Liquid Chromatography-mass Spectrometry. Biomed Chromatogr. 2023;37(3):e5564. PubMed PMID: 36509695.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous determination and quality evaluation of 16 compounds in Ligustri Lucidi Fructus covering different regions and processed products using ultra-high-performance liquid chromatography-mass spectrometry. AU - Li,Xiaoan, AU - Jia,Jianzhong, AU - Li,Tao, AU - Zhao,Zefeng, AU - Ren,Chaoxiang, AU - Liu,Haijing, AU - Pei,Jin, Y1 - 2023/01/03/ PY - 2022/11/04/revised PY - 2022/09/28/received PY - 2022/12/05/accepted PY - 2022/12/13/pubmed PY - 2022/12/13/medline PY - 2022/12/12/entrez KW - Ligustri Lucidi Fructus KW - multiparameter quantitative analysis KW - processed products KW - ultra-high-performance liquid chromatography-mass spectrometry SP - e5564 EP - e5564 JF - Biomedical chromatography : BMC JO - Biomed Chromatogr VL - 37 IS - 3 N2 - A quantitative analysis method and a chemical pattern recognition method were developed to evaluate raw Ligustri Lucidi Fructus (LLF) from different regions and different processed products. In this study, a comprehensive strategy using ultra-high-performance liquid chromatography-mass spectrometry quantitative analysis method was established for the simultaneous determination of 16 components in 47 batches of LLF covering 19 regions belonging to 8 provinces and 24 batches of different processed products (steamed LLF without auxiliary material, wine-steamed LLF, salt-steamed LLF, and vinegar-steamed LLF). The results of this study indicated that the proposed method was reliable and accurate for the rapid analysis proved by detection limit, quantification limit, precision, and accuracy. Furthermore, principal component analysis and hierarchical cluster analysis were employed to analyze the experimental data, showing that the best-quality samples of 47 batches of raw LLF were S47 (Lantian, Shaanxi), S39 (Pingyang-2, Shandong), S38 (Pingyang-1, Shandong), and S45 (Lingbao, Henan), whereas the worst-quality samples were S7-S16 (Huzhou, Zhejiang). In 24 batches of processed products, the best-quality samples were S48 (salt steamed 2 h), S60 (wine steamed 2 h), and S61 (wine steamed 4 h). Meanwhile, the heat map showed that the contents of triterpenoid saponins, including C16 (ursolic acid), C15 (oleanic acid), and C14 (maslinic acid), were higher than those of other compounds in 71 batches of samples. These results suggested that the quality of raw LLF in the central and northern regions was better than that in the southern regions, and regarding the processed products, different auxiliary materials had little effect on the quality of LLF, but steaming time of 2 h was appropriate. Briefly, this study proposed a multiparameter quantitative analysis method for the overall quality control of raw LLF samples covering different regions in China and different processed LLF. SN - 1099-0801 UR - https://www.unboundmedicine.com/medline/citation/36509695/Simultaneous_determination_and_quality_evaluation_of_16_compounds_in_Ligustri_Lucidi_Fructus_covering_different_regions_and_processed_products_using_ultra_high_performance_liquid_chromatography_mass_spectrometry_ DB - PRIME DP - Unbound Medicine ER -

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