Attempts to culture the parasitic stage of Dermatobia hominis (L. Jr.) in vitro (Diptera: Cuterebridae).
J Parasitol. 1987 Oct; 73(5):907-9.JP

Abstract

Dermatobia hominis larvae were cultured in a semidefined liquid medium. First-instar larvae (L1) grew well up to 44 days; 29.1% molted in a mean period of 8.62 days. Two larvae reached the third instar but lived only 1 and 18 days, respectively, after the second molt. The increase in size, measured in 4 larvae, was about 10-fold. Second- and third-instar larvae, obtained from the skin of cattle, survived and grew in the medium for up to 2 mo; 39.0% of the L2 molted while 77.3% of the L3 pupated, and some produced flies when transferred to sand after 14.84 +/- 10.08 days in the culture medium. Some maturation factor, obtained from the skin, may be necessary for the larvae to grow satisfactorily and to complete the full parasitic cycle in vitro.

Authors+Show Affiliations

Zeledón R

Escuela de Medicina Veterinaria, Universidad Nacional, Heredia, Costa Rica.

Silva S

No affiliation info available

MeSH

AnimalsCattleCattle DiseasesCulture MediaDipteraLarvaMyiasis

Pub Type(s)

Journal Article

Language

eng

PubMed ID

3656011

Citation

Zeledón, R, and S Silva. "Attempts to Culture the Parasitic Stage of Dermatobia Hominis (L. Jr.) in Vitro (Diptera: Cuterebridae)." The Journal of Parasitology, vol. 73, no. 5, 1987, pp. 907-9.

Zeledón R, Silva S. Attempts to culture the parasitic stage of Dermatobia hominis (L. Jr.) in vitro (Diptera: Cuterebridae). J Parasitol. 1987;73(5):907-9.

Zeledón, R., & Silva, S. (1987). Attempts to culture the parasitic stage of Dermatobia hominis (L. Jr.) in vitro (Diptera: Cuterebridae). The Journal of Parasitology, 73(5), 907-9.

Zeledón R, Silva S. Attempts to Culture the Parasitic Stage of Dermatobia Hominis (L. Jr.) in Vitro (Diptera: Cuterebridae). J Parasitol. 1987;73(5):907-9. PubMed PMID: 3656011.

* Article titles in AMA citation format should be in sentence-case

TY - JOUR T1 - Attempts to culture the parasitic stage of Dermatobia hominis (L. Jr.) in vitro (Diptera: Cuterebridae). AU - Zeledón,R, AU - Silva,S, PY - 1987/10/1/pubmed PY - 1987/10/1/medline PY - 1987/10/1/entrez SP - 907 EP - 9 JF - The Journal of parasitology JO - J Parasitol VL - 73 IS - 5 N2 - Dermatobia hominis larvae were cultured in a semidefined liquid medium. First-instar larvae (L1) grew well up to 44 days; 29.1% molted in a mean period of 8.62 days. Two larvae reached the third instar but lived only 1 and 18 days, respectively, after the second molt. The increase in size, measured in 4 larvae, was about 10-fold. Second- and third-instar larvae, obtained from the skin of cattle, survived and grew in the medium for up to 2 mo; 39.0% of the L2 molted while 77.3% of the L3 pupated, and some produced flies when transferred to sand after 14.84 +/- 10.08 days in the culture medium. Some maturation factor, obtained from the skin, may be necessary for the larvae to grow satisfactorily and to complete the full parasitic cycle in vitro. SN - 0022-3395 UR - https://www.unboundmedicine.com/medline/citation/3656011/Attempts_to_culture_the_parasitic_stage_of_Dermatobia_hominis__L__Jr___in_vitro__Diptera:_Cuterebridae__ DB - PRIME DP - Unbound Medicine ER -

Tags

Attempts to culture the parasitic stage of Dermatobia hominis (L. Jr.) in vitro (Diptera: Cuterebridae).J Parasitol. 1987 Oct; 73(5):907-9.JP