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Characterization of anti-liver kidney microsomal antibody in childhood autoimmune chronic active hepatitis: evidence for IgG1 subclass restriction, polyclonality and non cross-reactivity with hepatocyte surface antigens.
Clin Exp Immunol. 1987 Sep; 69(3):543-9.CE

Abstract

An indirect immunofluorescence technique was used to investigate the immunoglobulin class, IgG subclass, light chain type and complement fixing ability of anti-liver kidney microsomal antibody (anti-LKM) in the sera of six children and five adults with autoimmune chronic active hepatitis (aCAH). Anti-LKM titres ranged from 1/80 to 1/20,480. In the children, the antibody belonged to the IgG1 subclass alone (titre 1/80-1/20,480) and was able to fix complement (titre 1/40-1/5120). In the adult group, antibody belonged to the IgG1 subclass in three cases (titre 1/40-1/640) whilst two belonged to both IgG1 (titre 1/640) and IgG4 (titre 1/40, 1/640). Such subclass restriction is similar to that found in other autoimmune disorders and may be genetically determined. Investigation of the light chain constituent of anti-LKM revealed that the kappa to lambda ratio was consistent with a polyclonal antibody response. To investigate the nature of the antigen to which anti-LKM is directed, the ability of these sera to bind to the surface membrane of isolated human hepatoma cells (Alexander cells) was investigated. Four of the eleven sera showed significant binding activity. Prior incubation of these four sera with Alexander cells abolished their membrane binding activity, but did not alter the anti-LKM titre. These results suggest that anti-LKM binds to cytoplasmic constituents alone and does not cross-react with surface antigens.

Authors+Show Affiliations

Department of Immunology, King's College School of Medicine and Dentistry, London, UK.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

3665184

Citation

Peakman, M, et al. "Characterization of Anti-liver Kidney Microsomal Antibody in Childhood Autoimmune Chronic Active Hepatitis: Evidence for IgG1 Subclass Restriction, Polyclonality and Non Cross-reactivity With Hepatocyte Surface Antigens." Clinical and Experimental Immunology, vol. 69, no. 3, 1987, pp. 543-9.
Peakman M, Lobo-Yeo A, Mieli-Vergani G, et al. Characterization of anti-liver kidney microsomal antibody in childhood autoimmune chronic active hepatitis: evidence for IgG1 subclass restriction, polyclonality and non cross-reactivity with hepatocyte surface antigens. Clin Exp Immunol. 1987;69(3):543-9.
Peakman, M., Lobo-Yeo, A., Mieli-Vergani, G., Davies, E. T., Mowat, A. P., & Vergani, D. (1987). Characterization of anti-liver kidney microsomal antibody in childhood autoimmune chronic active hepatitis: evidence for IgG1 subclass restriction, polyclonality and non cross-reactivity with hepatocyte surface antigens. Clinical and Experimental Immunology, 69(3), 543-9.
Peakman M, et al. Characterization of Anti-liver Kidney Microsomal Antibody in Childhood Autoimmune Chronic Active Hepatitis: Evidence for IgG1 Subclass Restriction, Polyclonality and Non Cross-reactivity With Hepatocyte Surface Antigens. Clin Exp Immunol. 1987;69(3):543-9. PubMed PMID: 3665184.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of anti-liver kidney microsomal antibody in childhood autoimmune chronic active hepatitis: evidence for IgG1 subclass restriction, polyclonality and non cross-reactivity with hepatocyte surface antigens. AU - Peakman,M, AU - Lobo-Yeo,A, AU - Mieli-Vergani,G, AU - Davies,E T, AU - Mowat,A P, AU - Vergani,D, PY - 1987/9/1/pubmed PY - 1987/9/1/medline PY - 1987/9/1/entrez SP - 543 EP - 9 JF - Clinical and experimental immunology JO - Clin Exp Immunol VL - 69 IS - 3 N2 - An indirect immunofluorescence technique was used to investigate the immunoglobulin class, IgG subclass, light chain type and complement fixing ability of anti-liver kidney microsomal antibody (anti-LKM) in the sera of six children and five adults with autoimmune chronic active hepatitis (aCAH). Anti-LKM titres ranged from 1/80 to 1/20,480. In the children, the antibody belonged to the IgG1 subclass alone (titre 1/80-1/20,480) and was able to fix complement (titre 1/40-1/5120). In the adult group, antibody belonged to the IgG1 subclass in three cases (titre 1/40-1/640) whilst two belonged to both IgG1 (titre 1/640) and IgG4 (titre 1/40, 1/640). Such subclass restriction is similar to that found in other autoimmune disorders and may be genetically determined. Investigation of the light chain constituent of anti-LKM revealed that the kappa to lambda ratio was consistent with a polyclonal antibody response. To investigate the nature of the antigen to which anti-LKM is directed, the ability of these sera to bind to the surface membrane of isolated human hepatoma cells (Alexander cells) was investigated. Four of the eleven sera showed significant binding activity. Prior incubation of these four sera with Alexander cells abolished their membrane binding activity, but did not alter the anti-LKM titre. These results suggest that anti-LKM binds to cytoplasmic constituents alone and does not cross-react with surface antigens. SN - 0009-9104 UR - https://www.unboundmedicine.com/medline/citation/3665184/Characterization_of_anti_liver_kidney_microsomal_antibody_in_childhood_autoimmune_chronic_active_hepatitis:_evidence_for_IgG1_subclass_restriction_polyclonality_and_non_cross_reactivity_with_hepatocyte_surface_antigens_ L2 - https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/3665184/ DB - PRIME DP - Unbound Medicine ER -