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Genome-Wide Identification, Expression Analysis, and Potential Roles under Abiotic Stress of the YUCCA Gene Family in Mungbean (Vigna radiata L.).
Int J Mol Sci. 2023 Jan 13; 24(2)IJ

Abstract

YUCCA, belonging to the class B flavin-dependent monooxygenases, catalyzes the rate-limiting step for endogenous auxin synthesis and is implicated in plant-growth regulation and stress response. Systematic analysis of the YUCCA gene family and its stress response benefits the dissection of regulation mechanisms and breeding applications. In this study, 12 YUCCA genes were identified from the mungbean (Vigna radiata L.) genome and were named based on their similarity to AtYUCCAs. Phylogenetic analysis revealed that the 12 VrYUCCAs could be divided into 4 subfamilies. The evidence from enzymatic assays in vitro and transgenetic Arabidopsis in vivo indicated that all the isolated VrYUCCAs had biological activity in response to IAA synthesis. Expression pattern analysis showed that functional redundancy and divergence existed in the VrYUCCA gene family. Four VrYUCCAs were expressed in most tissues, and five VrYUCCAs were specifically highly expressed in the floral organs. The response toward five stresses, namely, auxin (indole-3-acetic acid, IAA), salinity, drought, high temperatures, and cold, was also investigated here. Five VrYUCCAs responded to IAA in the root, while only VrYUCCA8a was induced in the leaf. VrYUCCA2a, VrYUCCA6a, VrYUCCA8a, VrYUCCA8b, and VrYUCCA10 seemed to dominate under abiotic stresses, due to their sensitivity to the other four treatments. However, the response modes of the VrYUCCAs varied, indicating that they may regulate different stresses in distinct ways to finely adjust IAA content. The comprehensive analysis of the VrYUCCAs in this study lays a solid foundation for further investigation of VrYUCCA genes' mechanisms and applications in breeding.

Authors+Show Affiliations

Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China. Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing 210014, China.Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China. Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing 210014, China.Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China. Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing 210014, China.Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China. College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China. College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China. Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing 210014, China.Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China. Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing 210014, China.Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China. Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing 210014, China.Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China. Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing 210014, China.Institute of Industrial Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China. Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement, Nanjing 210014, China.

Pub Type(s)

Journal Article

Language

eng

PubMed ID

36675117

Citation

Wu, Ranran, et al. "Genome-Wide Identification, Expression Analysis, and Potential Roles Under Abiotic Stress of the YUCCA Gene Family in Mungbean (Vigna Radiata L.)." International Journal of Molecular Sciences, vol. 24, no. 2, 2023.
Wu R, Chen J, Lin Y, et al. Genome-Wide Identification, Expression Analysis, and Potential Roles under Abiotic Stress of the YUCCA Gene Family in Mungbean (Vigna radiata L.). Int J Mol Sci. 2023;24(2).
Wu, R., Chen, J., Lin, Y., Jia, Q., Guo, Y., Liu, J., Yan, Q., Xue, C., Chen, X., & Yuan, X. (2023). Genome-Wide Identification, Expression Analysis, and Potential Roles under Abiotic Stress of the YUCCA Gene Family in Mungbean (Vigna radiata L.). International Journal of Molecular Sciences, 24(2). https://doi.org/10.3390/ijms24021603
Wu R, et al. Genome-Wide Identification, Expression Analysis, and Potential Roles Under Abiotic Stress of the YUCCA Gene Family in Mungbean (Vigna Radiata L.). Int J Mol Sci. 2023 Jan 13;24(2) PubMed PMID: 36675117.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genome-Wide Identification, Expression Analysis, and Potential Roles under Abiotic Stress of the YUCCA Gene Family in Mungbean (Vigna radiata L.). AU - Wu,Ranran, AU - Chen,Jingbin, AU - Lin,Yun, AU - Jia,Qiyuan, AU - Guo,Yingjian, AU - Liu,Jinyang, AU - Yan,Qiang, AU - Xue,Chenchen, AU - Chen,Xin, AU - Yuan,Xingxing, Y1 - 2023/01/13/ PY - 2022/12/5/received PY - 2023/1/4/revised PY - 2023/1/11/accepted PY - 2023/1/21/entrez PY - 2023/1/22/pubmed PY - 2023/1/25/medline KW - YUCCA gene family KW - abiotic stress KW - expression profiles KW - identification KW - mungbean KW - transgene JF - International journal of molecular sciences JO - Int J Mol Sci VL - 24 IS - 2 N2 - YUCCA, belonging to the class B flavin-dependent monooxygenases, catalyzes the rate-limiting step for endogenous auxin synthesis and is implicated in plant-growth regulation and stress response. Systematic analysis of the YUCCA gene family and its stress response benefits the dissection of regulation mechanisms and breeding applications. In this study, 12 YUCCA genes were identified from the mungbean (Vigna radiata L.) genome and were named based on their similarity to AtYUCCAs. Phylogenetic analysis revealed that the 12 VrYUCCAs could be divided into 4 subfamilies. The evidence from enzymatic assays in vitro and transgenetic Arabidopsis in vivo indicated that all the isolated VrYUCCAs had biological activity in response to IAA synthesis. Expression pattern analysis showed that functional redundancy and divergence existed in the VrYUCCA gene family. Four VrYUCCAs were expressed in most tissues, and five VrYUCCAs were specifically highly expressed in the floral organs. The response toward five stresses, namely, auxin (indole-3-acetic acid, IAA), salinity, drought, high temperatures, and cold, was also investigated here. Five VrYUCCAs responded to IAA in the root, while only VrYUCCA8a was induced in the leaf. VrYUCCA2a, VrYUCCA6a, VrYUCCA8a, VrYUCCA8b, and VrYUCCA10 seemed to dominate under abiotic stresses, due to their sensitivity to the other four treatments. However, the response modes of the VrYUCCAs varied, indicating that they may regulate different stresses in distinct ways to finely adjust IAA content. The comprehensive analysis of the VrYUCCAs in this study lays a solid foundation for further investigation of VrYUCCA genes' mechanisms and applications in breeding. SN - 1422-0067 UR - https://www.unboundmedicine.com/medline/citation/36675117/Genome_Wide_Identification_Expression_Analysis_and_Potential_Roles_under_Abiotic_Stress_of_the_YUCCA_Gene_Family_in_Mungbean__Vigna_radiata_L___ DB - PRIME DP - Unbound Medicine ER -