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Two single-base-pair substitutions causing desensitization to tryptophan feedback inhibition of anthranilate synthase and enhanced expression of tryptophan genes of Brevibacterium lactofermentum.
J Bacteriol. 1987 Nov; 169(11):5330-2.JB

Abstract

A 5-fluorotryptophan-resistant mutant, termed 1041, was isolated from Brevibacterium lactofermentum AJ12036. The anthranilate synthase of 1041 was insensitive to feedback inhibition by tryptophan, and the specific activities of the anthranilate synthase and anthranilate phosphoribosyltransferase of 1041 were 29- and 23-fold higher than those in parental strain AJ12036, respectively. A single-base change (adenine to cytosine) that resulted in a Ser-to-Arg substitution was found in the trpE structural gene of 1041. This substitution was identified as the cause of the desensitization to feedback inhibition by tryptophan of anthranilate synthase in 1041. Another substitution (guanine to adenine) was found at a position in which a mutation would destabilize the rho-independent terminator structure within the putative attenuator. The enhanced synthesis of tryptophan enzymes in 1041 could be caused by this substitution in the attenuator.

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Authors+Show Affiliations

Matsui K
Central Research Laboratories, Ajinomoto Co. Inc., Kawasaki, Japan.
Miwa K
No affiliation info available
Sano K
No affiliation info available

MeSH

Amino Acid SequenceAnthranilate SynthaseBase CompositionBase SequenceBrevibacteriumFeedbackGenesGenes, BacterialMolecular Sequence DataMutationNucleic Acid ConformationOperonTranscription, GeneticTryptophan

Pub Type(s)

Journal Article

Language

eng

PubMed ID

3667535

Citation

Matsui, K, et al. "Two Single-base-pair Substitutions Causing Desensitization to Tryptophan Feedback Inhibition of Anthranilate Synthase and Enhanced Expression of Tryptophan Genes of Brevibacterium Lactofermentum." Journal of Bacteriology, vol. 169, no. 11, 1987, pp. 5330-2.
Matsui K, Miwa K, Sano K. Two single-base-pair substitutions causing desensitization to tryptophan feedback inhibition of anthranilate synthase and enhanced expression of tryptophan genes of Brevibacterium lactofermentum. J Bacteriol. 1987;169(11):5330-2.
Matsui, K., Miwa, K., & Sano, K. (1987). Two single-base-pair substitutions causing desensitization to tryptophan feedback inhibition of anthranilate synthase and enhanced expression of tryptophan genes of Brevibacterium lactofermentum. Journal of Bacteriology, 169(11), 5330-2.
Matsui K, Miwa K, Sano K. Two Single-base-pair Substitutions Causing Desensitization to Tryptophan Feedback Inhibition of Anthranilate Synthase and Enhanced Expression of Tryptophan Genes of Brevibacterium Lactofermentum. J Bacteriol. 1987;169(11):5330-2. PubMed PMID: 3667535.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Two single-base-pair substitutions causing desensitization to tryptophan feedback inhibition of anthranilate synthase and enhanced expression of tryptophan genes of Brevibacterium lactofermentum. AU - Matsui,K, AU - Miwa,K, AU - Sano,K, PY - 1987/11/1/pubmed PY - 1987/11/1/medline PY - 1987/11/1/entrez SP - 5330 EP - 2 JF - Journal of bacteriology JO - J Bacteriol VL - 169 IS - 11 N2 - A 5-fluorotryptophan-resistant mutant, termed 1041, was isolated from Brevibacterium lactofermentum AJ12036. The anthranilate synthase of 1041 was insensitive to feedback inhibition by tryptophan, and the specific activities of the anthranilate synthase and anthranilate phosphoribosyltransferase of 1041 were 29- and 23-fold higher than those in parental strain AJ12036, respectively. A single-base change (adenine to cytosine) that resulted in a Ser-to-Arg substitution was found in the trpE structural gene of 1041. This substitution was identified as the cause of the desensitization to feedback inhibition by tryptophan of anthranilate synthase in 1041. Another substitution (guanine to adenine) was found at a position in which a mutation would destabilize the rho-independent terminator structure within the putative attenuator. The enhanced synthesis of tryptophan enzymes in 1041 could be caused by this substitution in the attenuator. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/3667535/Two_single_base_pair_substitutions_causing_desensitization_to_tryptophan_feedback_inhibition_of_anthranilate_synthase_and_enhanced_expression_of_tryptophan_genes_of_Brevibacterium_lactofermentum_ DB - PRIME DP - Unbound Medicine ER -